Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Genetic Relationship of Cordyceps spp. Based on Internal Transcribed Spacer Sequences of Ribosomal DNA

Ribosomal DNA의 ITS 염기서열에 의한 동충하초속균의 유연관계

  • 남성희 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 황재삼 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 조세연 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 구태원 (농촌진흥청 농업과학기술원)
  • Published : 1999.12.01
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Abstract

The genetic relationships among six Cordyceps spp. were investigated based on internal transcribed spacer sequences of ribosomal DNA .A portion of the these genes was amplified by PCR. Approximately 590 base pairs were successfully amplified, cloned, sequenced, compared. The nucleotide sequence of the six amplified fragments were aligned by the clustal W program. As a result, Cordyceps militaris shared 87, 96, 98, 90 and 97% sequences homology with Paecilomyces japonica, Paecilomyces sp. J300, Paeciomyces farinosa. Paecilomyces sp. J500 and Cordyceps sinensis, respectively. Paecilomyces japonica also shared 87, 88, 92 and 87% sequence similarity with Paecilomyces sp. J300, Paecilomyces farinosa, Paecilomyces sp. J500 and Cordyceps sinensis, repectively.

Keywords

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