Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Identification of a Tandemly Repented DNA Sequence Using Combined RAPD and FISH in Welsh Onion (Allium fistulosum)

  • Bong Bo Seo (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Geum Sook Do (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Seon Hee Lee (Department of Biology, College of Natural Sciences, Kyungpook National University)
  • Published : 1999.03.01
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Abstract

A tandemly repeated DNA sequence was identified and characterized y the combined RAPD and FISH data from a total genomic DNA of Welsh onion (Allium fistulosum). A clone containing this repeating sequence was selected and sequenced. This repeating unit of 314 bp inserted into pAf 072 contained 54.1% adenine and thymine residues, and showed the primer sequence used, 5'-GAAACGGGTG-3', in both terminals of the sequence. Fluorescence in situ hybridization using this tandemly repeated sequence as a probe indicated that the detected sites were coincident with the major C-banded constitutive heterochromatin in the terminal regions of both arms of all 6 chromosomes.

Keywords

References

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