Abstract

Phylogenic relationships among the six species (D. testacea, D. sternopleuralis, D. bizonata, D. hisrrio, D. immigrans, D. curviceps) of the subgenus Drosophila in Korea were investigated by taximetrical analysis, allozyme and soluble proteins analysis. The taximetrical analysis of the 6 species according to the 30 morphological characters were performed using UPGMA. Two species D. bizonata and D. testacea clustered at high similarity enough to suggest that they were closely related species. A cladogram was constructed by cladistic analysis among 6 species with 30 morphological characters. These species were divided into two lines at their ancestors level: D. histrio, D. bizonata and D. testacea belonged to the one line, while D. immigrans, D. curviceps and D. sternopleuralis belonged to the other. The genetic relationships among the six species in the subgenus Drosophila species group were investigated following that eighteen loci were detected out of analysis fifteen allozymes by means of starch gel electrophoresis. Fum, Ao, Acph, Xdh, Me, Odh-2, G6pd and Sdh had monomorphic patterns in the each species, and specially Fum, Acph, Xdh, Sdh was same allele. The average genetic similarity of the six species which was belong to subgenus Drosophila in Korea was 0.531. Soluble proteins were analyzed by TDE. In genetic distance by TDE, D. immigrans and D. curviceps are even closer than any other ones showing genetic distance 0.264, D. bizonata and D. testacea are also closely related species. The results of taxonomical analysis, allozyme and soluble proteins analysis were similar. These results might suggest that the subgenus Drosophila consist of four group; the 1st group of D. immigrans, D. curviceps, 2nd group of D. bizonata, D. testacea, 3rd group of D. sternopleuralis group and 4th group of D. histrio group.

한국산 Drosophila아속 6종 (D. testacea, D. sternopleuralis, D. bizonata, D. hixtrio, D. immigrans, D. curviceps)의 계통관계를 알아보기 위하여 형태적분석, 동위효소분석 그리고 수용성 단백질 분석을 실시하였다. 30종류의 형태적 형질을 분석한 결과 D. bizonata와 D. testacea사이가 0.833의 유사지수를 나타내어 유연관계가 가장 높았다. 반면 D. immigrans와 D. bizonata사이의 유연관계가 가장 낮았다. 그리고 D.bizonata는 원시형질을 가장 많이 보유하고 있었으며, D. testacea가 가장 늦게 분지된 종으로 분석되었다. 15종류의 동위효소 분석에서는 18개의 loci가 검출되었다. 18개 loci중 Fum, Ao, Acph,Xdh, Me, Odh-5, G6pd, Sdh는 변이가 없었으며, 특히 Fum, Acph, Xdh, Sdh는 종간에도 변이가 없이 monomorphic하였다. 동위효소 분석에서는 D. immigrans와 D. curviceps사이가 가장 유사한 종으로 분석되었다. TDE에 의한 수용성 단백질 분석에서는 D. bizonata와 D. curvisceps사이가 가장 낮은 유연관계를 나타냈고, D. immigrans와 D. curviceps사이의 유연관계가 가장 높았다. 이상의 결과에서 한국산 Drosophila아속 6종은 D. immigrans, D. curviceps의 group, D. bizonata, D. testacea group, D. sternopleuralis group 그리고 D. histrio group으로 나눌 수 있다.

Keywords

• Drasophila;
• phylogenic relationships;
• morphology;
• allozyme;
• protein

• Drosophila아속;
• 유연관계;
• 형태;
• 동위효소;
• 단백질;