Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Expression of the UDP-Galactose-4-Epimerase Gene (galE) Constituting the gal/lac Operon of Lactococcus lactis ssp. lactis ATCC7962

  • Lee, Jung-Min, Choi, Jae-Yeon (Department of Food Science and Technology, Seoul National University) ;
  • Lee, Jong-Hoon (Department of Foods and Biotechnology, Kyonggi University) ;
  • Chang, Hae-Choon (Department of Foods and Biotechnology, Kyonggi University) ;
  • Chung, Dae-Kyun (Department of Food and Nutrition, Chosun University) ;
  • Kim, Jeong-Hwan (Institute and Department of Genetic Engineering, KyungHee University) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology, Gyeongsang National University) ;
  • Published : 1999.08.01
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Abstract

The gene (galE) encoding UDP-galactose-4-epimerase, operative in the galactose metabolic pathway, was cloned together with the $\beta$-galactosidase gene (lacZ) from Lactococcus lactis ssp. lactis ATCC7962 (L. lactis 7962). galE was found to have a length of 981 bps and encoded a protein with a molecular mass of 36,209 Da. The deduced amino acid sequence showed a homology with GalE proteins from several other microorganisms. A Northern analysis demonstrated that galE was constitutively expressed by its own promoter. When galactose or lactose was added into medium, the galE transcription was induced by several upstream promoters. The structure of the gal/lac operon of L. lactis 7962 was partially characterized and the gene order around galE was galT-lacA-lacZ-galE-orfX.

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References

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