Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Isolation and Identification of an Anaerobic Dissimilatory Fe(III)-Reducing Bacterium, Shewanella putrefaciens IR-1

  • Hyun, Moon-Sik (Water Environment Research Center, Korea Institute of Science and Technology) ;
  • Kim, Byung-Hong (Water Environment Research Center, Korea Institute of Science and Technology) ;
  • Chang, In-Seop (Water Environment Research Center, Korea Institute of Science and Technology) ;
  • Park, Hyung-Soo (Water Environment Research Center, Korea Institute of Science and Technology) ;
  • Kim, Hyung-Joo (Water Environment Research Center, Korea Institute of Science and Technology) ;
  • Kim, Gwang-Tae (Water Environment Research Center, Korea Institute of Science and Technology) ;
  • Kim, Mi-a (Water Environment Research Center, Korea Institute of Science and Technology) ;
  • Park, Doo-Hyun (Department of Biological Engineering, Seo Kyung University)
  • Published : 1999.01.01
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Abstract

In order to isolate a Fe(III)-reducer from the natural environment, soil samples were collected from various patty fields and enriched with ferric citrate as a source of Fe(III) under anaerobic condition. Since the enrichment culture was serially performed, the Fe(III)-reduction activity was serially diluted and cultivated on an agar plate containing lactate and ferric citrate in an anaerobic glove box. A Gram negative, motile, rod-shaped and facultative anaerobic Fe(III)-reducer was isolated based on its highest Fe(III)-reduction activity, Bacterial growth was coupled with oxidation of lactate to Fe(III)-reduction, but the isolate fermented pyruvate without Fe(III), The isolate reduced an insoluble ferric iron (FeOOH) as well as a soluble ferric iron (ferric citrate). Using the BBL crystal enteric/non-fermentor identification kit and 16S rDNA sequence analysis, the isolate was identified as Shewanella putrefaciens IR-1.

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