Screening and Chacterization of Bacteriocinogenic Lactic Acid Bacteria from Jeot-Gal, a Korean Fermented Fish Food

  • Published : 2000.06.01

Abstract

Bacteriocins are classified as proteins which are produced by heterogeneous groups of bacteria, having and antimicrobial effect of the closely related organisms. Recently, bacteriocins derived from lactic acid bacteria and other food-related organisms have been the subject of much research on potential food biopreservatives. The goal of this study was to screen and characterize the bacteriocinogenic lactic acid bacteria from Jeot-gal(commercial fermented fish foods). All bacteriocinogenic isolates were identified as lactic acid bacteria. Isolates NK24, NK34, and SA72 were tentatively identified as Lactobacillus brevis, according to the API 50 CHL kit database. All antimicrobial substances produced from four lactic acid bacteria isolates completely lost their antibacterial activity after being treated with some proteases, indicating to their proteinaceous nature. The bacteriocin produced from isolates NK24, NK34, and SA72 showed a broad spectrum of activity when compared to those produced from isolate SA131. All bacteriocins isolated during the course of this study showed a bactericidal mode of inhibition.

Keywords

References

  1. J. Food Sci. Nutr. v.2 Antibiosis and bacteriocin production of lactic acid bacteria isolated from Kimchi Bae, S.-S.;C. Ahn
  2. J. Dairy Sci. v.71 Inhibitory action of nisin against Listeria monocytogenes Benkerroum, N.;W. E. Sandine
  3. Bacteriocins of Lactic Acid Bacteria Bibek, R.;D. Mark.;A. D. Mark(ed.)
  4. J. Microbiol. Biotechnol. v.6 Isolation of Leuconostoc mesenteroides subsp. mesenteroides DU-0608 with antibacterial activity from Kimchi and characterization of its bacteriocin Cha, D. S.;D. M. Ha
  5. Bacteriocins of Lactia Acid Bacteria Screening methods for detecting bacteriocin activity Hoover, D. G.;S. K. Harlander;D. G. Hoover(ed.);L. R. Steenson(ed.)
  6. J. Food Sci. Nutr. v.2 Detection and identification of bacteriocins by propionibacteria isolated from commercial swiss cheese products Hur, J.-W.;N.-K. Lee;H.-Y. Lee;H.-D. Paik
  7. J. Bacteriol. v.167 Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481 Joerger, M. C.;T. R. Klaenhammer
  8. J. Microbiol. Biotechnol. v.3 Use of bacteriocinogenic Pediococcus acidilactici in sausage fermentation Kim, W. J.;S. S. Hong;S. K. Cha;Y. J. Koo
  9. J. Microbiol. Biotechnol. v.6 Screening of bacteriocinogenic lactic acid bacteria and their antagonistic effects in sausage fermentation Kim, W. J.
  10. Biochimie v.70 Bacteriocins of lactic acid bacteria Klaenhammer, T. R.
  11. Fish Fermentation Technology in Korea Lee, C.-H.;K. H. steinkraus(ed.);P. J. A. Reilly(ed.)
  12. Appl. Environ. Microbiol. v.63 Nisin resistance in Clostridium botulinum spore and vegetative cells Mazzotta, A. S.;A. D. Crandall;T. J. Montville
  13. Kor. J. dairy Sci. v.13 A study on the nisin producing plasmid of Lactococcus lactis 7962 Moon, Y. I.;Y. H. Chang;H. Y. Kim
  14. Food Microbiol. v.15 Characterization of antilisterial lactic acid bacteria isolated from Thai fermented fish products. Ostergaard, A.;P. K. B. Embarek;C. Wedell-Neergaard;H. H. Huss;L. Gram
  15. J. Ind. Microbiol. Biotechnol. v.19 Identification and partial characterization of tochincin, a bacteriocin produced by Bacillus thuringiensis subsp. tochigiensis Paik, H.-D.;S.-S. Bae;S.-H. Park;J.-G. Pan
  16. J. Food Sci. Nutr v.1 Bacteriocins: Assay, biochemistry, and mode of action Paik, H.-D.
  17. J. Microbiol. Biotechnol. v.6 Purification, characterization, and comparison of bacteriocins Paik, H.-D.;D.-W. Oh
  18. Biotechnol. Lett v.19 Isolation of a Lactococcus lactis strain wiht high resistance to nisin and increased nisin production Qiao, M.;M. J. Omaetxebarria, R. Ra;I. Oruetxebarria;P. E. J. Saris
  19. Arch. Microbiol. v.154 kCaseicin 80: Purification and characterization of a new bacteriocin from Lactobacillus casei Rammelsberg, M.;E. Muller;F. Radler
  20. Kor. J. Appl. Microbiol. Bioeng. v.15 Microbial characterization of gazami sik-hae fermentation Souane, M.;Y.-B. Kim;C.-H. Lee
  21. Bacteriol. Rev. v.40 Bacteriocin of Gram-positive vacteria Tagg, J. R.;A. S. Dajani;L. W. Wannamaker
  22. Bacteriocins of Lactic Acid Bacteria Antimicrobial proteins: Classification, nomenclature, diversity, and relationship to bacteriocins Thomas, J. M.;A. L. Kaiser;D. G. Hoover(ed.);L. R. Steenson(ed.)
  23. J. Microbiol. Biotechnol. v.5 Isolation and identification of Staphylococcus sp. from Korean fermented fish products Um, M.-N.;C.-H. Lee