Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biodegradation of Pyrene by the White Rot Fungus, Irpex lacteus

  • Hwang, Soon-Suk (Division of Biological Sciences, Kangwon National University) ;
  • Song, Hong-Gyu (Division of Biological Sciences, Kangwon National University)
  • Published : 2000.06.01
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Abstract

Abstract The removal percentage (94%) of 100 ppm of pyrene in a shaken culture of white rot fungus, Irpex lacteus, was much higher than that in a static culture (37.9%). Over 90% of the pyrene disappeared with I. lacteus grown at $15-27^{\circ}C$, yet less than 50% was removed at $37^{\circ}C$. The transformation rates of pyrene ($4.5-5.0{\;}\mu\textrm{g}/ml/day$) were not very different among cultures with 5- 30% inoculum sizes, and over 90% of the 100 ppm pyrene was removed in every case during 20 days of incubation. The biodegradation of pyrene by I. lacteus was confirmed by measuring the $CO_2$ evolved from the mineralization of the added pyrene. The activity of lignin peroxidase (LiP), which is known to be involved in the biodegradation by white rot fungi, was high between 8 to 12 days of incubation. Although manganese peroxidase activity was demonstrated during the same period as LiP, its activity was quite low, and no laccase activity was detected. Even though the activity patterns of ligninolytic enzymes did not coincide with the pyrene removal, this study shows that I. lacteus has a high biodegrading capability and can be a candidate for the bioremediation of polycyclic aromatic hydrocarbon contaminants.inants.

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References

  1. Stud. Mycol. v.33 A revision of ballistoconidia-forming yeasts and fungi Boekhout, T.
  2. The Yeasts, A Taxonomic Study, 4th ed. Sporobolomyces Kluyver & van Niel Boekhout, T.;T. Nakase;C. P. Kurtzman(ed.);J. W. Fell(eds.)
  3. Ph. D. Thesis. Computer-assisted classification and identification of actinomycetes Chun, J.
  4. Biochem. J. v.83 The qualitative analysis of the cell walls of selected species of fungi. Crook, E. M.;I. R. Johnston
  5. Antonie Van Leeuwenhoek v.74 Validation of the basidiomycetous yeast, Sporidiobolus microsporus sp. nov., based on phenotypic and molecular analyses Fell, J. W.;G. M. Blatt;A. Statzell-Tallman
  6. Int. J. Syst. Bacteriol v.49 Cystofilobasidiales, a new order of basidiomycetous yeasts Fell, J. W.;H. Roeijmans;T. Boekhout
  7. Rhodotorula F. C. Harrison;The Yeasts, A Taxonomic Study, 4th ed. Fell, J. W.;A. Statzell-Tallman;C. P. Kurtzman(ed.);J. W. Fell(eds.)
  8. Mol. Mar. Biol. Biotechnol. v.1 Partial rRNA sequences in marine yeasts: A model for identification of marine eukaryotes Fell, J. W.;A. Statzell-Tallman;M. J. Lutz;C. P. Kurtzman
  9. Evolution v.39 Confidence limits on phylogenies: An approach using the bootstrap Felsenstein, J.
  10. PHYLIP(Phylogeny inference package) version 3.5c. Felsenstein, J.
  11. J. Mol. Evol. v.16 A simple mehtod for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences Kimura, M.
  12. Antonie Van Leeuwenhoek v.63 The expanding realm of ballistosporous yeasts Nakase, T.;A. Takematsu;M. Hamamoto;M. Takashima
  13. CABIOS v.12 TREEVIEW: An application to display phylogenetic trees on personal computers Page, R. D. M.
  14. Mol. Biol. Evol. v.4 The neighbor-joining method: A new method for reconstruction of phylogenetic trees Saitou, N.;M. Nei
  15. Version 4. Sinauer Associates PAUP*. Phylogenetic analysis using parsimony(* and other methods) Swofford, D. L.
  16. FEBS Lett. v.338 Structure of the large ribosomal subunit RNA of Phytophthora megasperma, and phylogeny of the oomycetes Van der Auwera, G.;S. Chapelle;R. De Wachter
  17. Antonie van Leeuwenhoek v.54 Carbohydrate patterns of Candida, Cryptococcus and Rhodotorula species Weijman, A. C. M.;L. Rodriges de Miranda