Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Development of High Density Mammalian CellCulture system for the Production of Tissue-Type Plasminogen Activator

  • Published : 2000.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis menium ion. The semi-fed-batch operation was employer to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.

Keywords

References

  1. J. Biol. Chem. v.257 Fibrinolytic properties of one-chain and two-chain human extrinsic (tissue-type) plasminogen activator Rijken, D. C.;M. Hoylaerts;D. Collen
  2. J. Biol. Chem. v.257 Kinetics of the activation of plasminogen by human tissue plasminogen activator Hoylaerts, M.;D. C. Rijken;H. R. Lijnen;D. Collen
  3. Biochem. Biophys. Acta v.755 Plasminogen activation by tissue activator is accelerated in the presence of fibrinogen cyanogen bromide fragment FCB-2 Nieuwenhuizen, W.;J. H. Verheijen;A. Vermond;G. T. G. Chang
  4. Biochem. Biophys. Acta v.704 Studies on the kinetics of plasminogen activation by tissue plasminogen activator Ranby, M.
  5. J. Biol. Chem. v.114 The specific amino acid requirements of a mammalian cell in tissue culture Eagle, H.
  6. Adv. Appl. Microbiol. v.13 Large-scale cultivation of mammalian cells Telling, R. C.;P. J. Radlett
  7. Biotechnol. Bioeng. v.34 A structured kinetic modeling framework for the dynamics of hybridoma growth and monoclonal antibody production in continuous suspension culture Batt, B. C.;D. S. Kompala
  8. J. Biotechnol. v.1 The effects of glutamine utilization and ammonia production on the growth of BHK cells in microcarrier cultures Butler, M.;R. E. Spier
  9. Fed. Proc. v.43 Glutamine: a major energy source for cultured mammalian cells Zielke, H. R.;C. L. Zielke;P. T. Ozand
  10. Biochem. Biophys. Acta v.299 Role of glutamine in purine synthesis and in guanine nucleotide formation in normal fibroblasts and in fibroblasts deficient in hypoxanthine phosphoribosyl-transferase activity Raivio, K. O.;J. E. Seegmiller
  11. Bio/Technolgy v.6 Catabolic control of mammalian cell culture Glacken, M. W.
  12. Proc. Soc. Exp. Biol. Med. v.123 Amino acids and ammonia of fetal calf serum during storage Ryan, W. L.;C. Cardin
  13. J. Cell Physiol. v.80 Ammonia effects in cultures of normal and transformed 3T3 cells Visek, W. J.;G. M. Kolodny;P. R. Gross
  14. J. Immunol. Meth. v.86 Factors affecting cell growth and monoclonal antibody in stirred reactors Reuveny, S.;D. Velez;J. D. Macmillan;L. Miller
  15. Biotechnol. Bioeng. v.28 Reduction of waste product excretion via nutrient control: possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells Glacken, M. W.;R. J. Fleischaker;A. J. Sinskey
  16. Bioproc. Eng. v.3 Transient responses of hybridoma cells to lactate and ammonia pulse and step changes in continuous culture Miller, W. M.;C. R. Wilke;H. W. Blanch
  17. Proc. Soc. Exp. Biol. Med. v.107 Studies of inhibitory effects of ammonium ions in several virus-tissue culture systems Jensen, E. M.;O. C. Liu
  18. Virology v.13 Inhibitory effect of glutamine and ammonia on replication of influenza virus in ascites tumor cells Eaton, M. D.;A. R. Scala
  19. Proc. Soc. Exp. Biol. Med. v.111 Inhibitory effect of ammonia sulfate on Columbian SK virus propagation in mouse ascites tumor cells in vitro Furusawa, E.;W. Cutting
  20. Biotechnol. Bioeng. v.28 Reduction of waste product excretion via nutrient control: possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells Glacken, M. W.;R. J. Fleischaker;A. J. Sinskey
  21. J. Cell. Sci. v.12 The effects of adapting human diploid cells to grow in glutamic acid media on cell morphology, growth and metabolism Griffiths, J. B.
  22. Cytotechnology v.1 Establishment of Namalva cell lines which grow continuously in glutamine-free medium Hosoi, S.;H. Mioh;C. Anzai;S. Sato;N. Fujiyoshi
  23. Modern Approaches to Animal Cell Technology The use of glutamine-free media for the growth of three cell lines in microcarrier culture Hassell, T. E.;I. C. Allen;A. J. Rowley;M. Butler;R. E. Spier(ed.);J. B. Griffiths(ed.)
  24. Bio/Technology v.10 High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker Bebbington, C. R.;G. Renner;S. Thomson;D. King;D. Abrams;G. T. Yarranton
  25. Animal cell technology: Developments, process & products Growth study of lactate and ammonia double-resistant clones of HL-60 cells Schumpp, B.;E. J. Schlaeger;R. E. Spier(ed.);J. B. Griffiths(ed.);J. B. Macdonalds(ed.)
  26. Growth and Differentiation of Cells in Defined Environment Effects on cell proliferation of metabolites produced by cultured cells and their removal from culture in defined media Iio M.;A. Moriyama;H. Murakami;H. Murakami(ed.);I. Yamane(ed.);D. W. Barnes(ed.);J. P. Mather(ed.);I. Hayashi(ed.);G. H. Sato(ed.)
  27. Biotechnol. Tech. v.6 In situ removal of ammonium ions from hybridoma cell culture media: selection of adsorbent Jeong, Y. H.;S. S. Wang
  28. Kor. J. Biotechnol. Bioeng. v.11 Ammonium ion effects and its in situ removal by using immobilized adsorbent in hybridoma cell culture Jeong, Y. H.;H. I. Lee;G. T. Chun;I. H. Kim;S. S. Wang
  29. Animal Cell Technology: Basic & Applied Aspects v.8 Increase of hybridoma cell density and monoclonal antibody productivity by in situ removal of ammonium ion with immobilized adsorbent beads Kim, I. H.;Y. H. Jeong;G. T. Chun;S. S. Wang;K. Funatsu(et al.)(ed.)
  30. Korean J. Biotechnol. Bioeng. v.13 Development of an immobilized adsorbent for in situ removal of ammonium ion from mammalian cell culture media and its application to a mammalian cell bioreactor: Development of immobilized adsorbent system Park, B. G.;Y. W. Min;G. T. Chun;I. H. Kim;Y. H. Jeong
  31. Korean J. Biotechnol. Bioeng. v.13 Development of an immobilized adsorbent for in situ removal of ammonium ion from mammalian cell culture media and its application to a mammalian cell bioreactor: Application to cell culture system Park, B. G.;H. I. Rhee;G. T. Chun;I. H. Kim;Y. H. Jeong
  32. Cytotechnology v.18 Effect of am-hybridoma cells Matsumura, M.;F. R. P. Nayve
  33. Biotechnol. Bioeng. v.47 In-situ removal of ammonium and lactate through electrical means for hybridoma cultures Chang, Y. H. D.;A. J. Grodzinsky;D. I. C. Wang
  34. Biotechnol. Tech. v.3 Measurement of ammonia and glutamine in cell culture media by gas sensing electrodes Ozturk, S. S.;M. E. Meyerhoff;B. O. Palsson
  35. Chil. Chem. Acta v.141 A simple spectrophotometric assay of plasminogen activator: comparison with the fibrinolytic method Zaoui, D.;B. L. Fevre;H. Magdelenat;J. G. Bieth
  36. Bioproc. Eng. v.6 Growth inhibition of hybridoma cells by ammonium ion: correlation with effects on intracellular pH McQueen A.;J. E. Bailey
  37. Biotechnol. Bioeng. v.44 Biochemistry of growth inhibition by ammonium ions in mamalian cells Ryll, T.;U. Valley;R. Wagner
  38. Enzyme Microb. Technol. v.17 Role of glutamine in hybridoma cell culture: Effect on cell growth, antibody production and cell metabolism Jeong, Y. H.;S. S. Wang