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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antioxidative Effects of Cultivation of Streptomyces sp. BH-405 Isolated from Marine Origin

해양에서 분리한 Streptomyces sp. BH-405 배양액의 항산화 효과

  • Published : 2000.04.01
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Abstract

Antioxidative activity of c비ture of Streptomyces sp. BH-405 was investigated. After removal of pellets of Streptomyces sp. B BH-405, antioxidative substances were is미ated and suc$\infty$sively purified from culture of Streptomyces sp. BH-405 by by thin | layer chromatography $\pi$LC) or silica gel column chromatography. The fraction 3 obtained from ethylether fractionation of the C culture appeared highest level of anti oxidative activity as determined by thiocyanate method. Band 2 obtained by further P purification of this fraction showed higher anti oxidation level than that of same concentration of dl- $\alpha$ -tocopherol, butylated h hydroxy anisole (BHA). The band 2 showed higher rate of 1, 1.diphenyl 2-picrylhydrazyl (DPPH) decolorization than dl-$\alpha$-tocopherol. In the rat liver microsomes, band 2 rapidly inhibited lipid peroxidation which was initiated enzymatically by r reduced nicotinamide adenine dinucleotide phosphate (NADPH) or non-enzymatically by Fenton’s reagent. Band 2 inhibited on | lipid peroxidation of mitochondria or the linoleic acid hydro peroxide induced peroxidation system. It is concluded that band 2 obtained by fractionation of Streptomyces sp. BH-405 cultivation contained antioxidants with the capacity to inhibit oxidative m modification.

본 연구는 Streptomyces sp. BH-405의 배양액으로부터 유기용매로 추출하여 지질과 산화에 대한 항산화 효과를 실험하였다. Streptomyces sp. BH-405의 배양액을 ethylether로 추출한 후 silica gel column(100$\times$10 cm) chromatography로 분획한 획분을 POV로 측정한 결과 fraction 3에서 향산화 활성이 가장 높았다. 항산화 활성이 높은 fraction 3 획분을 다시 preparative TLC와 column chromatography로 더욱 정제하여 band 2를 얻었다. Band 2의 항산화 효과를 알아보기 위해 기존의 항산화제와 비교한 결과 이-$\alpha$-tocopherol, BHA보다 산화억제 효과가 높았으며 DPPH에 의한 수소 공여능은 실험결과가 우수하였다. 한편 band 2는 쥐 간의 microsome에 있어서 유리기의 소거기능이 있었고 NADPH 및 Fenton 시액에 위한 효소적 과산화 지질의 생성을 억제하였다. 그리고 mitochondria와 리놀산의 과산화 유도계에서도 지질의 과산화 과정에 생성하는 유리기의 형성을 차단하였다. 결론적으로 Streptomyces sp. BH-405의 배양액에서 얻은 획분인 band 2는 산화를 억제하는 활성이 있었다.

Keywords

References

  1. Chemical Industry v.41 no.7 Perspective of marine Biotechnology Miyachi, S.;Matsunaga, T.
  2. Chem. Ind. v.93 Bioactive metabolites of symbiotic marine microorganism Kobayashi, J.;Ishibashi, M.
  3. Chem, Rev v.93 The biosynthesis of marine natural products Garson, M. J.
  4. Matine natural products; Chemical and Biological perspective v.5 Barrow, K. D.;Scheuer, P.J.(ed.)
  5. Chemical and Industry v.41 no.7 Message to medicine from marine organisms Okami, Y.
  6. Lancet ii. Penicillin as a chemotherapeutic agent Chain, E. B.;Florey, H. W.;Gardner, A. D.;Heatley, N. G.;Jerrings, M. A.;Our-Ewing, J.;Sanders, A. G.
  7. J. Am. Oil Chem. Soc. v.52 Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene Branen, A.L.
  8. Korea, J. Food Sci. Technol v.23 no.3 Seperation of antioxidant compounds from edible marine algae Park, J. H.;Kang, K. C.;Baek, S. B.;Lee, Y. H.;Rhee, K. S.
  9. Korea J. Food Sci. Technol. v.26 no.4 Cho, S. Y.;You, B. H.Chang, M. H.;Lee, E. H.
  10. Korea J. Food Hyg. Effects of antioxidative activity of yeast against fish oil Ryu, B.H.;Kim, H. S.;Jeong, J. S.:Lee, S. H.
  11. Agric. Biol. Chem. v.46 no.9 Screening for antioxidant of microbial origin Aoyama, T.;Nakakita, Y.;Nakagawa, M.;Sakai, H.
  12. Biosci. Biotech. Biochem. v.58 no.10 A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria Takao, T.;Kitatani, F.;Watanabe, N.;Yagi, A.;Sakata, K.
  13. Biosci. Biotech. Biochem. v.57 no.6 Purification and characterization of the antioxidative substance produced by Aspergillus sojae K Harun-Or-Rachid, Ma.;Kato;Murata, A.;Kondok, M.
  14. Lebensm-Wiss. Technol. v.28 Antioxidative effect of crude antioxidant prepration from soybean fermented by Bacillus natto Hattori, T.;Ohishi, H.;Yokota, T.;Ohoami, H.;Watanabe, K.
  15. Biosci. Biotech. Biochem. v.59 no.2 Isolation of an antioxidant from Penicillium roquefortii IFO 5956 Hayashi, K.;Suzaki, K.;Kawaguchi, M.;Nakajima, T.;Suzaki, T.;Numata, M.;Nakamura, T.
  16. Ph.D Thesis Antioxidative activity of 2,6, dimethoxyphenol and its derivative produced from Bacillus sp. ks-96. Lee, Y. S.
  17. Biol. Chem. v.49 Thiocyanate method, Agric Fukan, Y.;Osawa, M.;Namiki, M.;Osaki, T.
  18. Nature v.181 Antioxidant determinations by the use of a stable free radical Blois, M.S.
  19. Method in Enzymolgy v.30 Microsomal lipid peroxidation Buege, J. A.;Aust, S. D.
  20. Toxicology of the liver Lipid peroxidation : biochemistry. measurement and significance in liver cell injury Rechnagel, R. O.;Glende, E. A.;Waller, R. L.;Lowreu, K.;PLAA, G.(ed.);Hewitt, W. R.(ed.)
  21. Biochem. Med. v.15 Simple fluometric assay for lipoperoxide in blood plasma Yaki, K. A.