KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Cultivation of a Saccharomyces cerevisiae in a Korean paper Digestion Wastewater

한지자숙폐액을 이용한 Saccharomyces cerevisiae의 배양

  • 이형춘 (Department of Food and Nutrition S대-won University)
  • Published : 2000.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A Saccharomyces cerevisiae isolated from a feed additive yeast product was cultivated in a Korean paper digestion wastewater in order to investigate the possibility of using it as substrate for the yeast. The yeast couldn't grow in the wastewater. It could grow in the wastewater diluted and the optimum dilution rate was 7.5 In batch cultivation with the jar fermenter the maximum total cell count was $1.34{\times}107/mL$ was obtained by the addition of undiluted digestion wastewater. By adding $(NH_4)_2S0_4 and KH_2P0_4$together with the undiluted wastewater the maximum cell concentration could be obtained faster.

한지자숙폐액을 사료첨가효모의 생산기질로 재이용할 수 있는 가를 검토하기 위하여 사료첨가효모제품으로부터 분리한 Sacch­a aromyces CereVlSlae종을 한지자숙폐액에 배양하였다. 자숙폐액원액에서는 균이 증식하지 못하였으나, 희석액에서는 증식하였으 며, 증류수로 7.5배 희석시 최대증식을 보였다. Jar fennenter배양 에서의 최대총균수는 $1.34{\times}107/mL$이었으며, 최대생균수는 $1.1{\times}107/mL$이었다. 배양액의 균체농도를 증가시키기 위하여 배양중 에 자숙폐액원액을 침가하여 총균수를 최대 $8.2{\times}107/mL$까지 증 가시킬 수 있었다. 지숙폐액원액과 함께 $(NH_4)_2S0_4 및 KH_2P0_4$ 까지 첨가함으로써 최대균체농도에 도달하는 시간을 단축시킬 수 있었다.

Keywords

References

  1. Theory and Practice of Making Korean Paper Chun. C
  2. Biomass: Production and Conversion(II) Cell Mass Production Nose, Y., K. Sibata;S. Kitani(Eds)
  3. American Society for Microbiology Manual of industrial microbiology and biotechnology Demain, A. L;N. A. Solomon
  4. Fundamentals of Biotechnology Prave, P., U. Faust, W. Sittig;D. A. Sukatscy
  5. The Canadian Journal of Chemical Engineering v.64 Mixed-Culture Microbial Protein from Waste Sulfite Pulping Liquor II : Its Production on Pilot-Plant Scale and Use in Animal Feed Lo, S. N;J. R. Moreau
  6. J. Chem. Tech. Biotechnol v.53 Effect of Temperature on Fermentation Kinetics of Waste Sulfite Liquor by Saccharomyces cerevisiae Rousseau, S., D. Rouleau, L. Yerushalmi;R. C. Mayer
  7. Animal Nutrition Yoon, H. S., W. J. Maeng, H. T. Shin, Y. K. Kim;T. S. Koh
  8. Biotech. Bioeng v.39 Design and Evaluation of Control Strategies for High Cell Densign and Evaluation of Control Strategies for High Cell Density Fermentations O'Conner, G. M., F. Sanchez-Riera;C. L. Cooney
  9. Biotechnology Letters v.3 no.10 Optimal Baker's Yeast Production in Extended Fed-Batch Culture by Using a Computer Coupled Pilot Fermenter Ramirez, A., A. Durand;H. T. Blachere
  10. J. Ferment. Bioeng v.74 no.1 Production of Streptomycin Using Computer-Controlled Fermentor: Addition of Glucose with Oxygen Uptake Rate and/or Carbon Dioxide Evolution Rate Monitoring Shimiz N., Y. Odaara;K. Kanto
  11. Biotech. Bioeng v.44 Continuous, Real-Time Monitoring of the Oxygen Uptake Rate(OUR) in Animal cell Bioreactors Yoon, S. J;K. B. Konstantinov
  12. Biotech. Bioeng v.35 Fast Inferential Adaptive Optimization of a Continuous Yeast Culture Based on Carbon Dioxide Evolution Rate Chang Y. K;H. C. Lim
  13. Biotechnology : Measuring Modelling and Control v.4 Methods and Instruments in Fermentation Gas Analysis Schugerl, K., H. J. Rehm;G. Reed(Eds)
  14. Bioprocess Monitoring and Control Pons, M. N
  15. Biotech. Bioeng v.19 Computer-Aided Bakers Yeast Fermentations Wang, H. Y., C. L. Cooney;D. I. C. Wang
  16. Biotech. Bioeng v.20 Microprocessor Control of Respiratory Quotient Whaite, P., S. Aborhey, E. Hong;P. L. Rogers
  17. Biotech. Bioeng v.21 Computer-control of Bakers Yeast Production Wang, H. Y., C. L. Cooney;D. I. C. Wang
  18. Biotechnology and Bioengineering Symp no.14 Computer Control of Fermentation Processes Shimizu, N., T. Sumitani;Y. Odawara