KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Enantioselective Production of Levofloxacin from Ofloxacin Butyl Ester by Porcine Liver Esterase

Porcine Liver Esterase를 이용한 광학선택적인 레보플록사신의 생산

  • 이상윤 (인하대학교 생물공학과) ;
  • 민병혁 (인하대학교 생물공학과) ;
  • 황성호 (인하대학교 생물공학과) ;
  • 구윤모 (인하대학교 생물공학과) ;
  • 이철균 (인하대학교 생물공학과) ;
  • 송성원 ((주)보령제약 중앙연구소) ;
  • 오선영 ((주)보령제약 중앙연구소) ;
  • 임상민 ((주)보령제약 중앙연구소) ;
  • 김상린 ((주)보령제약 중앙연구소)
  • Published : 2000.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this paper enantioselective production of levofloxacin by porcine liver esterase was investigated, To enhance the produc0-tivity various factors which affect the enzyme activity and the enantioselectivity were optimized, In terms of temperature and pH 45$^{\circ}C$ and 4.8 were found to be the best conditions for enzyme reaction. Addition of ofloxacin butyl ester the substrate at the concentration of 5 g/L was desirable to avoid the product inhibition and the activity of porcine liver esterase was maintained up to 72 hours.In addition to enhance the availability of substrate effect of solvent was also examined. It was found that the application of 5% (v/v) of acetone acetonitrile and dimethylsulfoxide did not increase the conversion of substrate and the presence of 5%(v/v) butanol inhibited the enzyme activity significantly.

Keywords