Morphological Characteristics of Bemisia tabaci(Gennadius) (Homoptera: Aleyrodidae) and Discrimination of Their Biotypes in Korea by DNA Makers

담배가루이 Bemisia tabaci(Gennadius)(Homoptera: Aleyrodidae)의 형태적 특징과 DNA 표식자에 의한 biotype 판별

  • Published : 2000.04.01

Abstract

The sweetpotato whiteflies, Bemisia tabaci(Gennadius), were found recently in Korea on Glycine max, Euphorbia pulcherrima, and Rosa hybrida. The biotype identity of Bemisia tabaci in Korea was determined by several DNA markers including the random amplified polymorphic DNAs, and restriction fragments length polymorphism of mitochondrial 12S and 16S rRNA genes. The electromorph profiles of DNA fragments from the rose(Jincheon) and poinsettia(Seoul) populations in Korea are both identical to those of B biotypes distributed in Australia, Israel, and Japan. The populations of B. tabaci collected on Glycine max, Ipomea batatas, and Perilla frutescens in different localities retained the same DNA markes with the population from Lonicera japonica and shikoku of Japan. These populations are non-B biotype and considered as an indigenous type in the Far Eastern Asia Region including Korea and Japan, Morphological Characteristics of B. Tabaci were also observed by the scanning electron microscope and described with the comparison to the other important whitefly pest, Trialeurodes vaporariorum (Westwood).

임의증폭 다형 DNA(RAPD)와 미토콘드리아 12S, 16S rRNA 유전자의 제한단편 DNA 표식자에 의해 한국에서 발생하는 담배가루이 개체군들의 biotype을 판별하였다. 진천의 장미 온실과 서울 내곡동의 포인세티아 온실에서 발생한 담배가루이는 일본, 이스라엘, 호주의 B biotype 과 동일한 DNA 단편들을 보유하였다. 여러 지역의 노지 콩 (Glycine max), 고구마 (Ipomea batatas), 들깨 (Perilla frutescens)에서 채집된 담배가루이 개체군들은 일본 시코쿠의 인동덩굴(Lonicera japonica)에서 채집된 담배가루이와 같은 DNA로 표식되었다. 이들 non-B biotype은 한국, 일본 등 극동아시아 지역에 고유한 계통으로 보인다. 최근 한국에서 발견된 담배가루이 Bemisia tabaci(Gennadius)의 주사전자현미경 관찰에 의한 형태적 특정을 온실 원예작물의 주요해충인 온실가루이 Trialeurodes vaporariorum (Westwood)와 비교하여 기재하였다.

Keywords

References

  1. Ann.Entomol.Soc.Am. v.87 Description of a species of Bemisia (Homoptera:Aleyrodidae) Bellows, T.S.;T.M.Perring;R.J.Gill;D.H.Headrick
  2. FAO Plant Prot.Bull. v.42 Current status of Bemisia tabaci as a plant pest and virus vector in agroecosystems worldwide Brown, J.K.
  3. Ann.Rev.Entomol. v.40 The sweetpotato or silverleaf whiteflies: biotypes of Bemisia tabaci or a species complex? Brown, J.K.;D.R.Frohlich;R.C.Rosell
  4. Phytopathology v.80 Variability in biological characteristics,isozyme patterns and virus transmission among populations of Bemisia tabaci Genn.in Arizona. Costa, H.S.;J.K.Brown
  5. Phytopathology v.83 Squash silverleaf symptoms induced by immature, but not adult. Bemisia tabaci Casta, H.S.;D.E.Ullman;M.W.Johnson;B.E.Tabashnik
  6. Insect Sci.Appl. v.14 Regional distribution,insecticide resistance, and reciprocal crosses between the A and B biotypes of Bemisia tabaci Costa, H.S.;J.K.Brown;S.Sivasupramaniam;J.Bird
  7. Aust.J.Entomol. v.36 Use of RAPD PCR to distinguish the B biotype from other biotypes of Bemisia tabaci(Gennadius)(Hemiptera:Aleyrodidae) De Barro, P.J.;Driver, F.
  8. Phylogenetic relationship of world populations of Bemisia tabaci (Gennadius) using ribosomal ITSI.Mol.Phyl.and Evol.(in press) De Barro, P.J.;F.Driver;J.W.H.Trueman;J.Curran
  9. Aust. J.Entomol. v.37 Distribution and identity of biotypes of Bemisia tabaci(Gennadius)(Hemiptera: Aleyrodidae) in member countries of the Secretariat of the Pacific Community De Barro, P.J.;W.Liebregts;M.Carver
  10. Mol.Ecol. v.8 A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial markers Frohlich, D.R.;I.Torres-Jerez;I.D.Bedford;P.G.Mardham;J.K.Brown
  11. Insect Mol.Biol. v.2 Characterization of differences between whiteflies using RAPD-PCR Grawel, N.J.;A.C.Bartlett
  12. Host plants in Bemisia tabaci-a literature survey on the cotten whitefly with an annotated bibliography Greathead, A.H.;M.J.W.Cock
  13. Proc.Natl.Acad.Sci.USA v.86 Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers Kocher, T.D.;W.K.Thomas;A.Meyer;S.V.Edwards;S.Paao;F.X.Villablanca;A.C.Wilson
  14. Whitefly of the world-a systematic catalogue of the Aleyrodidae(Homoptera) with host plant and natural enemy data Mound, L.A.;S.H.Halsey
  15. Occurrence of the sweetpotato whitefly,Bemisia tabaci(Gennadius)on the poinsettia.Plant Protection v.44 Ohto, K.
  16. J.Econ. Entomol. v.85 Identification of the poinsettia strain of Bemisia tabaci(Homoptera:Aleyrodidae) on Broccoli by electrophoresis Perring, T.M.;A.Cooper;D.J.Kazmer
  17. Science v.259 Identification of a whitefly species by genomic and behavioral studies Perring, T.M.;A.D.Cooper;R.J.Rodriguez;C.A.Farrar;T.S.Belleows
  18. Ann.Entomol.Soc.Am. v.87 Evolution,weighting,and phylogenetic utility of mitochondrial gene sequence and a compilation of conserved Polymerase chain reaction primers Simon, C.;F.Frati;A.Beckenbach;B.Crespi;H.Liu;P.Flook