KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characteristic of Carotenoid Component from Halophilic Bacteria, Haloarcular sp. EH-1

호염세균 Haloarcular sp. EH-1으로부터 추출한 카로테노이드 색소의 특성

  • 정영기 (동의대학교 미생물학과) ;
  • 최병대 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 강석중 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 정성훈 (경상대학교 해양생물이용학부/해양산업연구소) ;
  • 이용규 (동서대학교 식품공학부) ;
  • 김해윤 (동의대학교 미생물학과) ;
  • 정명주 (동의대학교 미생물학과)
  • Published : 2000.12.01
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Abstract

In order to identification of carotenoid pigments of Haloarcular sp. EH-1 as a food for fish were analyzed. The content of carotenoids cultured in 3 L and 5 L bioreactor were 83.1 and 82.7 mg%, respectively. Identification of each carotenoid was achieved by means of co-TLC and co-HPLC with authentic specimens, spectroscopic and instrumental analyses, and chemical treatments as usual. The main components identified were ${\beta}$-carotene(8.1%), 3-hydroxyechinenone(42.0%) and astaxanthin(25.0%).

호염성 세균이 갖는 특성 즉, 다른 균에 의한 오염 감소, 세포벽 파괴의 수월성, 용매를 이용하지 않는 색소추출 등을 응용하기 위한 기초 자료를 얻기 위하여 호염성 세균인 Haloarcular sp. EH-1 으로부터 추출한 천연색소를 bioreactor 에서 배양한 후 색소의 함량을 조사한 결과 3 L 용량에서는 8 83.1 mg%, 5 L 용량에서는 82.7 mg%의 카로테노이드를 함 유하는 것으로 나타났다 이들의 주된 성분은 $\beta$-c따otene (8.1 %), $\beta$-hydroxyechinenone (42.0%) 및 astaxanthin(25.0% )으로 동정 되었으며, 이들 성분을 동정하기 위하여 표품과의 co- TLC, co-HPLC, 가시부 홉수스펙트럼, 화학적 변화 등을 조사하였다.

Keywords

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