Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Phylogenetic Analysis of Hepatitis B Virus Genome Isolated from Korean Patient Serum

  • Kim, Seon-Young (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang, Hyen-Sam (Department of Microbiology, College of natural Sciences, Seoul National University) ;
  • Kim, Yeon-Soo (Laboratory of Cell Biology, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2000.12.01
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Abstract

The complete nucleotide sequence of hepatitis B virus DNA isolated from Korean patient serum was determined and characterized, and its phylogenetic relation was then investigated. The viral genome was 3,215 base pairs long and included four well known open reading frames (i.e. surface antigens, core antigens, X protein and DNA polymerase). The sequence of the surface antigen showed that the HBV genome under investigation, designated HBV 315, was characteristic of subtype adr. A phylogenetic analysis using the total genome sequence revealed that HBV315 was grouped into genomic group C together with isolates from Japan, China, Thailand, Polynesia, and New Caledonia. The mean percent similarity between HBV315 and other HBV isolates in genomic group C was 97.25%, and that with other genomic groups ranged from 86.16% to 91.25%. The predicted amino acid sequences of HBV315 were compared with two closely related subtype adr isolates, M38636 and D12980. The results showed that the X gene product was identical in the three strains, while there were significant amino acid sequence differences between HBV315 and M38636 in the Pre-S1 and Pre-S2 regions.

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References

  1. FEBS Lett. v.453 Human hepatitis B virus mutants: Significance of molecular changes Chen, W. N.;C. J. Oon
  2. J. Korean Med. Sci. v.14 Analysis of the precore and core promoter DNA sequence in liver tissues from patients with hepatocellular carcinoma Cho, S. W.;Y. J. Shin;K. B. Hahm;J. H. Jin;Y. S. Kim;J. H. Kim;H. J. Kim
  3. J. Virol. v.71 Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2 Chow, Y. H.;W. L. Huang;W. K. Chi;Y. D. Chu;M. H. Tao
  4. J. Virol. v.73 Intracellular retention of hepatitis B virus surface proteins reduces interleukin-2 augmentation after genetic immunizations Geissler, M.;V. Bruss;S. Michalak;B. Hockenjos;D. Ortmann;W. B. Offensperger;J. R. Wands;H. E. Blum
  5. J. Microbiol. Biotechnol. v.8 Expression and characterization of recombinant E2 protein of Hepatitis C Virus by insect cell/Baculovirus expression system Han, B. K.;B. Y. Lee;M. K. Min;K. H. Jung
  6. J. Microbiol. Biotechnol. v.10 Phylogenetic analysis of genus Sporobolomyces based on partial sequences of 26S rDNA Hong, S. G.;J. S. Chun;J. S. Nam;Y. D. park;K. S. Bae
  7. Vaccine v.17 Immunization with a plasmid encoding a modified hepatitis B surface antigen carruing the receptor binding site for hepatocytes Hui, J.;M. Mancini;G. Li;Y. Wang;P. Tiollais;M.L. Michel
  8. J. Gen. Virol. v.75 no.Pt6 Genetic conservation within subtypes in the hepatitis B virus pre-S2 region [published erratum appears in J. Gen. Virol. 1995 Mar;76(Pt3):727] Kidd-Ljungren, K.;A. M. Courouce;M. Oberg;A. H. Kidd
  9. J. Gen. Virol. v.76 no.Pt9 The hepatitis B virus X gene: Analysis of functional domain variation and gene phylogeny using multiple sequences Kidd-Ljunggren, K.;M. Oberg;A. H. Kidd
  10. J. Microbiol. Biotechnol. v.9 Large-scale culture of Hepatitis A Virus in human diploid MRC-5 cells and partial purification of the ciral antigen for use as a vaccine Kim, H. S.;Y. J. Chung;Y. J. Jeon;S. H. Lee
  11. Korean Biochem. J. v.21 Complete nucleotide sequence of Hepatitis B Virus (subtype adr) Kim, K.-T.;Sang Won Hyun;Yong Sok Kim;Hyune Mo Rho
  12. Korean Biochem. J. v.17 Cloning and expression of Hepatitis B Virus surface antigen gene Kim, Y.-S.;Hyen-Sam, Kang
  13. Korean J. Intern. Med. v.10 Association of the core clustering mutations (codon 21-34) and the severity of chronic hepatitis B in Korean patients Koh, K. C.;H. S. Lee;C. Y. Kim
  14. Proc. Natl. Acad Sci. USA v.95 Isolation of a hepadnavirus from the woolly monkey, a New World primate Lanford, R. E.;D. Chavez;K. M. Brasky;R. B. Burns(3rd);R. Rico-Hesse
  15. J. Virol. v.74 Hepadnavirus infection in captive gibbons lanford, R. E.;D. Chavez;R. Rico-Hesse;A. Mootnick
  16. Virology v.260 Muscle-specific expression of hepatitis B surface antigen: No effect on DNA-raised immune responses Loirat, D.;Z. Li;M.Mancini;P. Tiollais;D. Paulin;M. L. Michel
  17. J. Virol. v.74 Detection of hepatitis B virus infection in wild-born chimpanzees (Pan troglodytes verus):Phylogenetic relationships with human and other primate genotypes MacDonald, D. M.;E. C. Holmes;J. C. Lewis;P. Simmonds
  18. Clin. Microbiol. Rev. v.12 Update on diagnosis, management, and prevention of hepatitis B virus infection Mahoney, F. J.
  19. Virology v.198 Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes Norder, H.;A. M. Courouce;L. O. Magnius
  20. Arch. Virol. Suppl. v.8 Complete nucleotide sequences of six hepatitis B viral genomes encoding the surface antigen subtypes ayw4, adw4q-,and adrq- and their phylogentic classification Norder, H.;A. M. Courouce;L. O. Magnius
  21. J. Gen. Virol. v.73 no.Pt5 Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains Norder, H.;B. Hammas;S. Lofdahl;A. M. Courouce;L. O. Magnius
  22. Virus Res. v.39 Relationships between serotypes and genotypes of hepatitis B virus: Genetic classification of HBV by use of surface genes Ohba, K.;M. Mizokami;T. Ohno;K. Suzuki;E. Orito;J. Y. Lau;Y. Ina;K. Ikeo;T. Gojobori
  23. J. Microbiol. Biotechnol. v.9 Purification and characterization of recombinant Hepatitis C virus replicase Park, C. H.;Y. H. Kee;J. H. Lee;J. H. Oh;J. C. Park;H. J. Myung
  24. Vaccine v.17 DNA-mediated immunization to hepatitis B virus envelope proteins: preS antigen secretion enhances the humoral respones Prange, R.;M. Werr
  25. Nucleic Acids Res. v.17 The nucleotide sequence and reading frames of a mutant hepatitis B virus subtype adr Rho, H. M.;K. Kim;S. W. Hyun;Y. S. Kim
  26. J. Gen. Virol. v.81 A new genotype of hepatitis B virus: Complete genome and phylogenetic relatedness Stuyver, L.;S. De Gendt;C. Van Geyt;F. Zoulim;M. Fried;R. F. Schinazi;R. Rossau
  27. PAUP*. Phylogenetic Analysis Using parsimony(*and other methods), (4.0 ed.) Swofford, D. L.
  28. J. Virol. v.73 A new group of hepadnaviruses naturally infecting orangutants (Pongo pygmaeus) Warren, K. S.;J. L. Heeney;R. A. Swan;Heriyanto;E. J. Verschoor
  29. J. Med. Vorol. v.59 A novel hepatitis B virus variant S 129 (Gln → Leu): Lack of correlation between antigenicity and immunogenicity Wu, L.;J. W. He;X. Yao;H. M. Liu;Y. M. Wen