Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Receptor-Mediated Endocytosis of Hepatitis B Virus PreS1d Protein in EBV-Transformed B-Cell line

  • Park, Jung-Hyun (Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Eun-Wie (Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Dong-Gun (Research Center for Proteineous Materials, Chosun University) ;
  • Park, Jung-Min (Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Yun-Jung (Department of Oral Anatomy, College of Dentistry, Seoul National University) ;
  • Choi, Eun-A (Department of Microbiology and Immunology, University of Illinois at Chicago) ;
  • Kim, Kill-Lyong (Protein Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2000.12.01
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Abstract

The specific binding and internalization of viral particles is an essential step for the successful infection of viral pathogens. In the case of the hepatitis B virus (HBV), virions bind to the host cell via the preS domain of the viral surface antigen and are subsequently internalized by endocytosis. HBV-preS specific receptors are primarily expressed on hepatocytes, however, viral DNA and proteins have also been detected in extrahepatic sites, suggsting that celluar recepators for HBV may also exist on extrahepatic cells. Recently, an EBV-transformed B-cell line was identified onto which the preS region binds in a receptor-ligand specific manner. In this study, this specific interaction was further characterized, and the binding region within the preS protein was locaized. Also the internalization after host cell attachment was visualized and analyzed by fluorescence-labeled HBV-preS1 proteins using confocal microscopy. Energy depletion by sodium azide treatment effectively inhibited the internalization of the membrane-bound preS1 ligands, thereby indicating an energy-dependent receptor-mediated endocytotic pathway. Accordingly, the interaction of HBV-pres! with this specific B-cell line may serve as an effective model for an infection pathway in extrahepatic cells.

Keywords

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