Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Structural Study of Antisense Dimers, Modified Adenosine-Thymidine Phosphorothioate

  • Jung, Kyeong-Eun (AgroPharma Research Institute, Dongbu Research Council) ;
  • Yang, Mi-Rim (AgroPharma Research Institute, Dongbu Research Council) ;
  • Lee, Kwang-Jun (AgroPharma Research Institute, Dongbu Research Council) ;
  • Lim, Hong (AgroPharma Research Institute, Dongbu Research Council) ;
  • Jung, Ji-Hyun (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Lim, Yoon-Gho (Department of Applied Biology and Chemistry, Konkuk University) ;
  • Cho, Youl-Hee (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Shin, Dong-Hoon (Graduate School of Biotechnology, Korea University) ;
  • Lee, Chul-Hoon (Department of Medical Genetics & Institute of Biomedical Science, College of Medicine, Hanyang University)
  • Published : 2000.12.01
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Abstract

Antisense molecules are structurally simple linear oligomers of nucleotides. They can recognize a complementary sequence by base pairing, therefore, antisense drugs composed of 15-16 bases are potentially useful, unlike drugs such as protein agonists, antagonists, and inhibitors. Since antisense oligomers are classified as nucleotides, they are subject to attack by nucleases. In order to be antisense drugs resistant to degradation by nucleases, the structural modifications in the linkages, bases, and sugars to satisfy this requirement are considerable. We attempted in this study, to synthesize 16-mer antisenses with a modified linkage and adenosine. When studying on the three-dimensional structure of the oligomer, however, the existence of isomers may complicate the interpretation of the NMR data. Therefore, an attempt was made to eliminate the above problem, thus, two dimers were synthesized and their structural studies were carried out.

Keywords

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