The Change of Attached Bacterial Community on Aggregates in Lake Paldang

팔당호에서 Aggregates에 부착한 세균군집구조의 변화

  • Published : 2000.12.01

Abstract

For scrutinizing the changes of aggregated bacteria in Lake Paldang, the FISH method was applied by using the rhodamine labeled probes, and total bacteria, chloropyll a concentrations and nutrients concentrations were measured. The aggregates were collected with sediment traps. The T-N, T-P, chlorophyll a concentrations of aggregates were higher 5-15 times, 81-140 and 49-66 times than water samples, respectively. Also, the bacterial numbers of aggregates were 200 times higher than those of water smaples. The ratios of each groups of water sample were 2.1-7.4% for $\alpha$-group, 4.5-8.3% for $\beta$-group, 2.1-7.4% for $\gamma$-group, 2.1-6.1% for Cytophaga-Flavobacterium group and 0.1-2.5% for 'other'group, respectively. While, in aggregates, the ratios of $\alpha$-, $\beta$-, $\gamma$- and Cytophaga-Flavobacterium groups were very small and most abundant group was 'other' bacteria. With these results, the aggregated bacteria in Lake Paldang had a particular group composition of bacteria.

팔당호에서 fluorescent in situ hybridization(FISH) 방법을 이용하여 aggregates에 부착한 세균군집의 변화를 조사하였다. 조사대상은 Eubacteria에 속하는 세균과 Class Proteobacteria에 속하는 세균중 $\alpha$-, $\beta$-, $\gamma$ -group과 Cytophaga-Flavobacterium group이었고, 환경요인의 변화를 파악하고자 영양염류와 엽록소 a를 측정하였다. Aggregate와 물시료의 조사항목을 비교하면, TN의 경우 5~15배, TP는 81~140배, 엽록소 a는 49~66배로 aggregate가 높게 나타났으며, 총 세균수 역시 물시료에서 전체적으로 1.0~$2.0{\times}10^6$cells.$ml^{-1}$이었고, aggregates 부착세균에서 0.2~~$3.6{\times}10^8$cells.$ml^{-1}$의 범위로 물시료보다는 aggregates에 부착한 세균의 밀도가 200배 높았다. 또 수심별로는 5m trap 보다 20m trap에서 더 많은 수가 측정되었다. 총 세균수에 대한 세균군집구조의 비율은 부유세균의 경우 $\alpha$-group이 4.5~8.3%, $\beta$-group이 2.2~8.0%, ${\gamma}$-group이 2.1~7.4%, Cytophaga-Flavobacterium group이 2.1~6.1% 'Other'group은 0.1~2.5로 매우 낮았으나 aggregate에 부착한 세균의 군집구조는 $\alpha$-, $\beta$-, $\gamma$-group과 Cytophaga-Flavobacterium group이 아닌 'Other'group이 약 10.2~32.1%로 우점하는 경향을 보였다. 이처럼 팔당호에서 aggregates에 부착한 세균의 군집구조는 부유세균과 비교해볼 때 독특한 군집구조를 나타내었다.

Keywords

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