YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of Daidzein and Genistein on Immune Function in Mice

Daidzein과 Genistein이 생쥐의 면역 기능에 미치는 영향

  • 은재순 (우석대학교 약학대학) ;
  • 조선경 (우석대학교 약학대학) ;
  • 권진 (우석대학교 약학대학) ;
  • 서은실 (우석대학교 약학대학) ;
  • 전훈 (우석대학교 약학대학) ;
  • 염정열 (우석대학교 약학대학)
  • Published : 2000.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

High soy consumption leading to high exposures of soy isoflavones has been associated with a reduced risk of cancers at many sites. As part of a study focusing on the chemopreventive mechanisms, we have investigated the modulating effects of daidzein and genistein, a prominent and more bioavailable isoflavone in soy foods, on murine immune function. Daidzein (50 mg/kg) or genistein (50 mg/kg) was administered p.o. once a day for 7 days in BALB/c mice. Daidzein decreased the mitogen-stimulated proliferation of murine splenocyte, but genistein increased it. Daidzein stimulated the secretion of interleukin-4, but inhibited the secretion of ${\gamma}$-interferon, interleukin-2 and tumor necrosis factor-$\alpha$. Genistein stimulated the secretion of ${\gamma}$-interferon, interleukin-2 and tumor necrosis factor-$\alpha$, but inhibited the secretion of interleukin-4. Daidzein and geiustein inhibited the production of nitric oxide and enhanced the phagocytic activity in peritoneal macrophage. These results suggest that cancer preventive effects of daidzein is partly concerned with the secretion of $T_{H}$2 cells cytokine and the activation of macrophage phagocytosis, and genistein is partly concerned with the secretion of $T_{H}$l cells cytokine, tumor necrosis factor-$\alpha$ and the activation of macrophage phagocytosis.sis.

Keywords

References

  1. Scand.J.Clin.Lab.Invest v.50 Western diet and western diesease; some hormonal and biochemical mechanism and associations Adlercreurz,H.
  2. Environ.Helath Perspect v.103 no.7 Phytoestrogens;epidemiology and a possible role in cancer protection Adlercreurz,H
  3. JNCI v.83 The role of soy products in reducing risk of cancer Messina,M.;Barnes,S.
  4. Nutr.Cancer v.21 Soy intake and cancer risk;a review of in vitro and in vivo data. Messina,M.,Persky,V.,Setchell,KDR,;Barnes.S
  5. Nutr.Cancer v.21 Soy intake and cancer risk;a review of in vitro and in vivo data Barnes,S.,Grubbs,C.,Setchell,KDR,;Carlson,J.
  6. Nutrition and Cancer v.30 no.1 Effect of isoflavones genistein and daidzein in the inhibition on jung Metastasis in mice induced by B16F-10 melanoma cells Menon,L.G.,Kuttan,R.,Nair,M.G.,Chang,Y.C.;Kutten,G
  7. Nutrition and Cancer v.30 no.3 Effect of soy-derived isoflavonoids on the induced growth of MCF-7 Cells by estrogenic enviromental chemicals Verma, S.P.and Goldin,B.R
  8. Cancer Research v.58 Inhibition of murine bladder tumorigenesis by soy isoflavones via alterations in the cell cycle,apoptosis and andgio-genesis Zhou,J.R.,Mukherjee,P.,Gugger,E.T.,Tanaka,T.,Blackburn,L.;Clinton,S.K
  9. Jpn.J.Clin.Oncol v.28 no.6 Effects of soybean isoflavones on cell growth and apoptosis of the human prostatic cancer cell line LNCaP Onozawa,M.,Fukuda,K.,Ohtani,M.,Akaza,H.Sugimura,T.;Wakabayashi,K.
  10. Arterioscler Thromb.Vasc.Biol v.17 no.11 Genistein,the dietary-derived angiogenesis inhibitor,prevents LDL oxidation and Protects endotherial cells from damage by atherogenesis LDL Kapiotis,S.,Hermann,M.,Held,I.,Seelos,C.,Ehringer,H.;Gmeiner,B.M.K
  11. J.Pharm.& Exper.Ther v.280 no.2 Direct block of voltage-sensitive sodium channels by genistein,a tyrosine kinase inhibitor Paillart,C.,Carlier,E.,Guedin,D.,Dargent,B.;Couraud,F
  12. Am.J.Clin.Nitr v.40 Nonsteroidal estrogens of dietary origin;Possible roles in hormone-dependent disease, Setchell,K.D.R.,Borriello,S.P.,Hulme,P.,Kirk,D.N.;Axelson,M
  13. J.Biol.Chem v.262 Genistein,a specific inhibitor of tyrosine-specific protsin kinase. Akiyama,T.,Ishida,J.,Nakagawa,S.,Ogawara,H.,Wantanabe,S.I.
  14. Methods Enzymol v.201 Use and specificity of genistein as inhibitor of protein-tyrosine kinases Akiyama,T.;Ogawara,H
  15. Cancer Detect.Prev v.9 Immunological enhancement by fat-soluble vitamins,minerals,and trace metals:a factor in cancer prevention Watson,R.R.
  16. Crit,Care Med v.18 Dietary immunomodulation:beneficial effects on oncogenesis and tumor growth Lowell,J.A.,Parnes,H.L;Blackburn,G.L
  17. Semin.Oncol.Nurs v.8 The role of the immune system in the pathogenesis of cancer Appelbaum,J.W.
  18. Endocr.Rev v.5 Regulation of the immune system by sex steroids Grossman,C.J.
  19. Science v.227 Interactions between the gonadal steroids and the immune system Grossman,C.J
  20. Ann.Med v.26 Hormonal regulation of immune respinses Paavonen,T
  21. Nultr.Cancer v.29 Enhancement of immune function in mice fed high doeses of daidzein Zhang,R.,Li,Y.;Wang,W
  22. Nutr.Cancer v.29 no.1 Individual and combinatory effects of soy isoflavones on the in vitro potentiation of lymphocyte activation Wang.W.Higuchi,C.M;Zhang,R.
  23. Proc.Natl.Acad.Sci.USA v.75 Planning for lymphocytes : A method for cell selection Wysocki,L.J.;Sato,V.L
  24. P388D1.J.Immunol v.120 Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line Mizel,S.B.,Openheim.J.J.;Rosensteich,D.L.
  25. J.Immunol.methods v.65 Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxic assays. Mosmann,T
  26. J.Immunol,methods v.129 A simple and rapaid method to determine hematopoietic growth factor activity Kotnic,V.;Fleischmann,W.R.Jr
  27. J.Immunol.Methods v.174 Chemiluminenscence and nitrite determinations by the MALU macrophage cell line Boudard,F.,Vallot,N.,Cabaner,C.;Bastide,M
  28. J.Immunol.Methods v.112 Measurement of phagocyte chemiluminen-scence in a microtiter plate format. Blair,A.L.,Cree,I.A.,Beck,J.S.;Hating,M.J.G
  29. J.Immuno,Methods v.60 Differentiation between attached and ingested immune complexes by a fluroescence quenching cytofluorometric assay. Sahlin,S.,Hed,J.;Rundquist,I.
  30. J.Immuno.Methods v.162 Arapid and simple micro-fluorometric phagocytosis assay Chok.P.W.,Choon,S.P.;Benjamin,H.S
  31. Infec.Immunity v.59 no.9 Killing of plasmodium faciparum in vitro by nitric oxide derivatives Rockett,K.A.,Awburn,M.M.,Cowden,W.B.;Clark,I.A
  32. Cellular and molecular immunology.2ed.Saunders, Abbas,A.K.,Lichtman,A.H.;Pober,J.S
  33. Br.J.Exp.Pathol v.65 Albumin inhibits human polymorphonuclear leukocyte luminol-dependent chemiluminescence evidence for oxygen radical scavenging. Holt,M.E.,Ryall,M.E.T.;Campbell,A.K.
  34. Immun v.45 no.1 Intra-and extracellular events in luminol-dependent chemiluminescence of polymorphonuclear leukocytes.Infect Breiheim,G.,Stendahl,O.;Dahlgren,C
  35. CRC Press,Boca Raton,FL. The cortical actin filament network of neutrophil leukocytes during phagocytosis and chemotaxis Sheterline,P.;Rickard,J.E.
  36. J.Cell Biol v.110 no.5 Cytosolic free calcium elevation mediates the phagosome-lysosme fusion during phagocytosis in human neutrophils Jaconi,M.E.E.,Lew,D.P.,Carpentier,J.L.Magnusson,K.E.,Sjogren,M.;Stendahl,O
  37. Biochem,and Biophysical Research Communications v.233 Nitric oxide-releasing particles inhibit phagocytosis in human neutrophils Forslund,T.;Sundqvist,T
  38. Korean.J.Immunol v.18 Nitric oxide inhibits macrophage pseudopodia formation in the activated marcrophages Jun,C.D.,Park,S.K.,Kim,J.M.,Kim,J.D.;Chung,H.T
  39. FASEB.J v.6 Nitric oxide as a secretory product of mammalian cells Nathan,C