Experimental and Molecular Medicine

Korean Society for Biochemistry and Molecular Biongy (생화학분자생물학회)
권호 표지

Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root

Lee, Si-Eun;Ju, Eun-Mi;Kim, Jeong-Hee

  • Published : 2001.12.31

⟨ Previous Next ⟩

Abstract

The extract from Smilax china root has been used as medicinal remedy and reported to retain antimicrobial and antimutagenic acitivities. In this study, a possible presence of antioxidant activity of Smilax china root extract was investigated. Methanol ex-tract (Me) revealed the presence of high 1,1-diphenyl- 2-picrylhydrazyl (DPPH) free radical scavenging activity ($IC_{50}\;7.4\;{\mu}g/ml$) and protective property of cell's viability. Further fractionation with various solvent extraction and assay showed high levels of DPPH free radical scavenging activity in the ethyl acetate, butanol and water extracted fractions. In addition, V79-4 cells treated with Me of Smilax china root induced an increase of superoxide dismutase, catalase and glutathione peroxidase activities in a dose-dependent manner between $4-100\;{\mu}g/ml$. These results suggest that the medicinal component of the root of Smilax china extracts also contains antioxidant activity.

Keywords

References

  1. Anal Biochem v.44 Assays of superoxide dismutase Beauchamp, C.;Fridovich, I.
  2. J Ethnopharmacol v.74 Anti-oxidant effect of Withania somnifera glycowithanolides in chronic footshock stress-induced perturbations of oxidative free radical scavenging enzymes and lipid peroxidation in rat frontal cortex and striatum Bhattacharya, A.;Ghosal, S.;Bhattacharya, S.K.
  3. Br Med Bull v.43 Free radicals in biological systems: a review oriented to the inflammatory process Blake, D.R.;Allen, R.E.;Lunee, J.
  4. Nature v.181 Antioxidant determinations by the use of a stable free radical Blosi, M.S.
  5. Free Radic Res Commu v.12 Free radical processes in multistage carcinogenesis Borek, C.
  6. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.M.
  7. Free Radic Res v.29 Luteolin-rich artichoke extract protects low density lipoprotein from oxidation in vitro Brown, J.E.;Rice-Evans, C.A.
  8. Free Radic Biol Med v.22 Antioxidant and prooxidant behaviour of flavonoids: Structure-activity relationships Cao, G.;Sofic, E.;Prior, R.L.
  9. Free Radic Biol Med v.26 Inhibition of peroxinitrite-dependent DNA base modification and tyrosine nitration by the extra virgin olive oilderived antioxidant hydroxytyrosol Deiana, M.;Aruoma, O.I.;Bianchi, M.;Halliwell, B.;Aeschbach, R.;Corongiu, F.P.
  10. J Am Oil Chem Soc v.74 Antioxidant activity of mung bean hulls Duh, P.D.;Yen, W.J.;Du, P.C.;Yen, G.C.
  11. Nature v.408 Oxidants, oxidative stress and the biology of ageing Finkel, T.;Holbrook, N.J.
  12. Free Radic Res v.33 mRNA expression profile of a human cancer cell line in response to Ginkgo biloba extract: induction of antioxidant response and the Golgi system Gohil, K.;Moy, R.K.;Farzin, S.;Maguire, J.J.;Packer, L.
  13. Free Radicals in Biology and Medicine Halliwell, B.;Gutteridge, J.M.C.
  14. J Immunol Methods v.119 Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill Hansen, M.B.;Nielsen, S.E.;Berg, K.
  15. Cancer Lett v.150 Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng Keum, Y.S.;Park, K.K.;Lee, J.M.;Chun, K.S.;Surh, Y.J.
  16. Exp Mol Med v.28 Effect of ginsenoside $Rb_1$ on lipid peroxidation and neurotoxicity induced by MPTP in liver and brain of mouse Kim, J.S.;Oh, J.H.;Lee, D.W.;Park, H.J.;Park, D.K.
  17. Mol Cell Biochem v.203 Antioxidant properties of a North American gingseng extract Kitts, D.D.;Yuan, Y.V.;Wijewickreme, A.N.;Hu, C.
  18. Mutat Res v.204 Antimutagenic activity of extracts from anticancer drugs in Chinese medicine Lee, H.;Lin, J.Y.
  19. J Agric Food Chem v.48 Evaluation of the antioxidant and pro-oxidant effects of tea catechin oxypolymers Li, C.;Xie, B.
  20. Phytother Res v.14 Screening for antioxidant properties of Salvia reflexa hornem Malencic, D.;Gasic, C.;Popovic, M.;Boza, P.
  21. J Biol Chem v.244 Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprein) McCord, J.M.;Fridovich, I.
  22. Anal Biochem v.95 Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction Ohkawa, H.;Ohishi, N.;Yagi, K.
  23. J Lab Clin Med v.70 Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase Paglia, D.E.;Valentine, W.N.
  24. Exp Mol Med v.33 The protective effect of Amomum xanthoides extract against alloxan-induced diabetes through the suppression of $NF{\kappa}B$ activation Park, B.H.;Park, J.W.
  25. Ann NY Acad Sci v.695 Free radicals in the genesis of Alzheimer’s disease Richardson, S.J.
  26. J Agric Food Chem v.45 Antioxidant properties of catechins and green tea extracts in model food emulsions Roedig-Penman, A.;Gordon, M.H.
  27. Phytomed v.7 Chemomodulatory action of Aloe vera on the profiles of enzymes associated with carcinogen metabolism and antioxidant status regulation in mice Singh, R.P.;Dhanalakshmi, S.;Rao, A.R.
  28. J Korean Soc Food Sci Nutr v.27 Antimicrobial activity and composition of extract from Smilax china root Song, J.H.;Kwon, H.D.;Lee, W.K.;Park, I.H.
  29. J Agric Food Chem v.44 Total oxidant capacity of fruits Wang, H.;Cao, G.;Prior, R.L.
  30. J Agric Food Chem v.48 Correlation of antioxidant capacities to oxygen radical scavenging enzyme activities in blackberry Wang, S.Y.;Jiao, H.
  31. J Agric Food Chem v.48 Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen Wang, S.Y.;Jiao, H.
  32. Br Heart J v.69 The role of oxidized low density lipoproteins in atherogenesis Witztum, J.L.