A Comparison of Feeding Lactobacillus and Virginiamycin Influence on Performance and Intestinal Microflora of Broiler Chicks

유산균과 버지니아마이신의 급여가 육계의 생산성 및 장내 미생물에 미치는 영향

  • 김상호 (축산기술연구소 가금과) ;
  • 박수영 (축산기술연구소 가금과) ;
  • 유동조 (축산기술연구소 가금과) ;
  • 이상진 (축산기술연구소 가금과) ;
  • 류경선 (전북대학교 동물자원과학과)
  • Published : 2001.04.01

Abstract

This experiment was conducted to investigate the effect of feeding two strains of Lactobacillus and virginiamycin on performance, nutrients digestibility and intestinal microflora of broiler chicks(Abor acres$\times$Abor Acres) were randomly allocated into six treatments with four replications for five weeks. Control(no supplement), 0.05% virginiamycin(VM), Lactobacillus crispatus avibro1(LC), Lactobacillus reuteri avibro2(LR), LC+0.05% VM(LC+VM), LR+0.05% VM(LR+VM) were supplemented into basal diets, which contained ME 3,100kcal/kg and CP 22.0, 20.0% for starting and finishing period, respectively. Weight gain, feed intake and feed conversion(FC) were weekly measured. Nutrients digestibility, intestinal microflora and fecal noxious gas were examined at the end of experiment. Weight gains of chicks fed Lactobacillus or VM was significantly higher than control(P〈0.05). Feed intake increased significantly in those supplemental groups(P〈0.05). FC of chicks fed Lactobacillus or VM significantly lower than control(P〈0.05). Degestibility of crude protein, calcium, and phosphorus improved significantly in alone or combined Lactobacillus treatments(P〈0.05). Whereas DM, crude fat and ash digestibility were not statistically different. Feeding Lactobacilli tended to increase the total Lactobacillus spp. in ileum at one and three weeks of age(WOA) and showed significantly higher in cecum than control at 5 WOA. Total yeast were not shown difference at 1 and 3 WOA, but significantly increased at 5 WOA(P〈0.05). The ileal and cecal anaerobes were started to increase from the first WOA. Fecal NH$_3$gas tended to decrease in Lactobacillus treatments compared to that of other treatments.

Keywords

References

  1. Br. Poult. Sci. v.37 The influence of Lactobacillus acidophilus and bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk Abdulrahim, S.M.;Haddadin, S.Y.;Hashlamoun, E.A.;Robinson, R.K.
  2. Avian. Dis. v.24 Effect of ingested Lactobacilli on Salmonella infantis and Escherichia coli and on intestinal flora, pasted vents, and chick growth Adler, H.E.;DaMassa, A.J.
  3. Official methods of analysis(16th ed.) AOAC
  4. Poult. Sci. v.70 The role of intestinal microflora on the prevention of Salmonella colonization in gnotobiotic chickens Baba, E.;Nagaishi, S.;Fukata, T.;Arakawa, A.
  5. Poult. Sci. v.64 Influence of virginiamycin on phosphorus utilization by broiler chicks Buresh, R.E.;Miles, R.D.;Harms, R.H.
  6. Poult. Sci. v.65 A differential response in turkey poults to various anibiotics in diets designed to be deficient or adequate in certain essential nutrients Buresh, R.E.;Harms, R.H.;Miles, R.D.
  7. Antibiot. Chemother. v.5 A preliminary report on antibiotic number 899. De Sommer, P.;Van Dijck, P.
  8. Poult. Sci. v.57 Lactobacillus cultures in broiler diets Dilworth, B.C.;Day, E.J.
  9. Poult. Sci. v.61 Effects of virginiamycin on Leghorn-type pullets fed optimal and sub-optimal protein levels Douglas, C.R.;Miles, R.D.;Harms, R.H.
  10. Amer. J. Dis. Child. v.105 Staphylococci and infection immunity Dubos, R.J.
  11. Annales de recherches Veterinaires v.14 Implantation and development of the gut flora in the newborn animal Ducluzeau, R.
  12. Poult. Sci. v.72(Suppl.1) Lactobacillus reuteri immunomodulation of stressor-associated disease in newly hatched chickens and turkeys Dunham, H.J.;William, C.;Edens, F.W.;Casas, I.A.;Dobrogosz, W.J.
  13. Poult. Sci. v.42 Effect of antibiotics on growth and nutrient absorption of chicks Eyssen, H.V.;De Sormer, P.
  14. Poult. Sci. v.41 The growthpromoting action of virginiamycin and its influence on the crop flora in chickens Eyssen, H.V.;De Prins;De Sormer, P.
  15. Br. Poult. Sci. v.13 A practical evaluation of five feed additives likely to be used as growth promoters in broiler rations Foster, W.H.
  16. Poult. Sci. v.57 Interrelationship of Lactobacillus and zinc bacitracin in diets of turkey poults Francis, C.;Janky, D.M.;Arafa, A.S.;Harms, R.H.
  17. J. Appl. Bacteriol. v.36 Ecological studies on the Lactobacillus flora associated with the crop epithelium of the fowl Fuller, R.
  18. J. Appl. Bacteriol. v.66 Probiotics in man and animals Fuller, R.
  19. Am. J. Clin. Nutr. v.27 Lactobacilli which attach to the crop epithelium of the fowl Fuller, R.;Brooker, B.E.
  20. Poult. Sci. v.65 Influence of virginiamycin of broiler fed four levels of energy Harms, R.H.;Ruiz, N.;Miles, R.D.
  21. In Physiology and Biochemistry of the Domestic Fowl The influence of the intestinal microflora on nutrition Jayne-Williams, D.J.;Fuller, R.;Bell, D.J.;Freeman, B.M.
  22. Letters in Applied Microbiology v.23 Antagonistic effects of intestinal Lactobacillus isolates on pathogens of chicken Jin, L.Z.;Ho, Y.W.;Abdullah, N.;Ali, M.A.;Jalaludin, S.
  23. Poult. Sci. v.77 Growth performance, intestinal microbial populations, and serum cholesterol of broiler fed diets containing Lactobacillus cultures Jin, L.Z.;Ho, Y.W.;Abdullah, N.;Jalaludin, S.
  24. Poult. Sci. v.57 Growth rate, feed conversion and dietary metabolizable energy in response to virginiamycin supplementation of different diets March, B.E.;Soong, R.;MacMillan, C.
  25. Proc. 41st Annu. Florida Poult. Inst. The protein sparing ability of virginiamycin Miles, R.D.
  26. Nutr. Rep. Int. v.30 Influence of virginiamycin in pullets and broilers fed diets containing suboptimal protein and sulfur amino acid levels Miles, R.D.;Douglas, C.R.;Harms, R.H.
  27. Poult. Sci. v.63 Virginiamycin and broiler performance Miles, R.D.;Douglas, C.R.;Harms, R.H.
  28. Animal Feed Science and Technology v.61 Nutrient retention and production parameters of Single Comb White Leghorn layers fed diets with varying crude protein levels and supplemented with directfed microbials Nahashon, S.N.;Nnkaue, H.S.;Mirosh, L.W.
  29. Animal Feed Science and Technology v.57 Performance of Single Comb White Leghorn layers fed a dier with a live microbial during the growth and egg laying phases Nahashon, S.N.;Nnkaue, H.S.;Mirosh, L.W.
  30. Nutrient requirements of poultry(9th ed.) National Research Council
  31. Poult. Sci. v.42 Studies on the stimulation of growth by dietary antibiotics Nelson, F.E.;Jensen, L.S.;McGinnis, J.
  32. Am. J. Clin. Nutr. v.69(suppl.) Developmental microbial ecology of the neonatal gastrointestinal tract Roderick I mackie;Abdelgnani Sghir;H Rex Gaskins
  33. SAS/STAT Software for PC. Release 6.12 SAS Institute
  34. Annual Reviews of Microbiology v.31 Microbial ecology of the gastrointestinal tract Savage, D.C.
  35. J. Exp. Med. v.127 The gastrointestinal epithelium and its autochthonous bacterial flora Savage, D.C.;Dubos, R.;Schaedler, R.W.
  36. Poult. Sci. v.77 Effect of BROILACT on the physicochemical conditions and nutrient digestibility in the gastrointestinal tract of broilers Schneitz, C.;Kiiskinen, T.;Toivonen, V.;Nasi, M.
  37. Poult. Sci. v.52 Influence of the implantation of Lactobacillus acidophilus in chicks on the growth, feed conversion, malabsorption of fats syndrome and intestinal flora Tortuero, F
  38. Poult. Sci. v.54 The influence of intestinal(cecal) flora on serum and egg yolk cholesterol levels in laying hens Tortuero, F.;Brenes, A.;Rioperez, J.
  39. J. Ani. Sci. v.46 The mode of growth promotion by antibiotic Visek, W.J.
  40. Poult. Sci. v.70 Influence of virginiamycin on growth and feed efficiency of Large White turkeys Waibel, P.E.;Halvorson, J.C.;Noll, S.L.;Hoffbeck, S.L.
  41. Poult. Sci. v.61 In vivo effects of Lactobacillus acidopilus against pathogenic Escherichia coli in gnotobiotic chicks Watkins, B.A.;Miller, B.F.;Neil, D.H.
  42. Poult. Sci. v.76 Effect of feeding diets containing an antibiotic, a probiotic, or yucca extract on growth and intestinal urease activity in broiler chicks Yeo, J.M.;Kim, K.I.
  43. 한국가금학회지 v.27 육계 생산성 및 맹장내 미생물에 대한 유산균의 첨가 효과 김상호;박수영;유동조;나재천;최철한;박용윤;이상진;류경선
  44. 한국축산학회지 v.36 항생제, 효소제, 효모제, 생균제 및 β-agonist가 육계의 성장과 영양소 이용율에 미치는 효과 노선호;이찬호;최윤제;한인규
  45. 대한수의학회지 춘계 심포지움 v.37 no.1 송아지 세균성 설사증 윤용덕
  46. 농업논문집 v.35 사료에 항생제 및 생균제 첨가가 육계의 생산성에 미치는 영향 이상진;김삼수;서옥석;나재천;이상헌;정선부
  47. 한축지 v.26 생균제제의 성장촉진 효과에 관한 연구. Ⅰ. 브로일러에 대한 Lactobacillus sporogenes의 성장촉진효과와 분변 및 장내 세균총의 변화에 미치는 영향 한인규;이상철;이지희;이금기;이정치
  48. 한축지 v.26 생균제제의 성장촉진 효과에 관한 연구. Ⅱ. 브로일러에 대한 Clostridium butyricum ID의 성장촉진효과와 분변 및 장내 세균총의 변화에 미치는 영향 한인규;이상철;이진희;김정대;정필근;이정치