Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biodegradation of Hydrocarbons by an Organic Solvent-Tolerant Fungus, Cladosporium resinae NK-1

  • Oh, Ki-Bong (Natural Products Research Institute, Seoul National University) ;
  • Mar, Woong-Chon (Natural Products Research Institute, Seoul National University) ;
  • Chang, Il-Moo (Natural Products Research Institute, Seoul National University)
  • Published : 2001.02.01
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Abstract

A kerosene fungus of Cladosporium resinae NK-1 was examined for its ability to degrade individual n-alkanes and aromatic hydrocarbons by gas chromatography-mass spectrometry, and its organic solvent-tolerance was investigated by making use of the water-organic solvent suspension culture method. It grew on a wide range of solvents of varying hydrophobicities and it was found to have tolerance to various kinds of toxic organic solvents (10%, v/v) such as n-alkanes, cyclohexane, xylene, styrene, and toluene. A hydrocarbon degradation experiment indicated that NK-1 had a greater n-alkane degrading ability compared to that of the other selected strains. C. resinae NK-1, which could utilize 8-16 carbon chain-length n-alkanes of medium chain-length as a carbon source, could not assimilate the shorter chain-length n-alkanes and aromatic hydrocarbons tested so far. The n-alkane degrading enzyme activity was found in the mycelial extract of the organism.

Keywords

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