Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Cloning and Characterization of the ${\beta}-Galactosidase$ Gene from Bifidobacterium adolescentis Int57

  • Park, Myeong-Soo (Research Center for Agriculture and Life Sciences, Seoul National University) ;
  • Yoon, Hyeon-Jin (Department of Food Science and Technology, Seoul National University) ;
  • Rhim, Seong-Lyul (Department of Genetic Engineering, Hallym University) ;
  • Ji, Geun-Eog (Department of Food Science and Nutrition, Seoul National University)
  • Published : 2001.02.01
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Abstract

A ${\beta}-galactosidase$ gene of Bifidobacterium adolescentis Int57 (INT57) was cloned using the shotgun method. The sequence of the ${\beta}-galactosidase$ gene existing in the sequenced 3,260-bp fragment showed higher than 40% homology with other bacterial ${\beta}-galactosidase$ genes. The expression in Escherichia coli suggested that the ${\beta}-galactosidase$ might have a monomeric, dimeric, or tetrameric protein structure. This is probably the first peer-reviewed sequence analysis of the ${\beta}-galactosidase$ gene of the genus Bifidobacterium.

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