Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparison of the Chemotaxis Potential of Bacteria Isolated from Spinach Roots and Nonrhizosphere Soil

  • Kim, Jong-Shik (Molecular Genetics Division, National Institute of Agricultural Science and Technology) ;
  • Sakai Masao (Laboratory of Soil Microbiology and Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University) ;
  • Lee, Si-Kyung (Department of Applied Biology and Chemistry, Faculty of Agriculture and Life Science, Konkuk University) ;
  • Yahng, Chahng-Sook (Department of Applied Biology and Chemistry, Faculty of Agriculture and Life Science, Konkuk University) ;
  • Matsuguchi Tatsuhiko (Laboratory of Soil Microbiology and Biochemistry, Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University)
  • Published : 2001.02.01
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Abstract

In order to investigate the role of bacterial chemotaxis in root colonization, the chemotaxis potential of bacteria isolated from spinach roots was compared with that of bacteria from nonhizosphere soil, with reference to the plant age (1,000 isolates), soil moisture conditons (1,400 isolates), and part of the root (200 isolates). The % CT (% occurrence of chemotaxis (+) isolates among total bacterial isoltes) of the root isolates significantlyfluctuated during the plant growth period, reaching a maximum after 10-15 days of growth. At this time period, the maximum % CT for the root isolates was around 70-80% CT under a soil moisture 50% WFP (% volume of water-filled pores in total soil pores), and then gradually reduced with an increasing % WFP. The results of the chemotaxis potential of each of the 100 islates from the spinach roots and nonrhizosphere soil under various % WFP demonstrated that the % CT of the root isolates were significantly higher than those of solates from the nonrhizosphere soil under a wide range of soil moisture content (35-80% WFP). Furthermore, the % CT value (80%) from the upper root was significantly higher than tht (55%) from the lower root. Compared with the % CT values of the roots, the values from the nonrhizosphere soil did not significantly vary relative to the plant age of % WFP. These results indicate that chemotaxis would appear to be a major factor in bacterial root colonization.

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