Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Characterization of Monoclonal Antibody to Glycoprotein D Antigen of Herpes simplex Virus Type 2

  • Choi, Young-Sook (Department of Clinical Pathology, Konkuk University) ;
  • Kim, Tae-Un (Department of Clinical Lab. Science, Catholic University of Pusan) ;
  • Lee, Inyung-Hoan (Department of Biological Science Konkuk University) ;
  • Cho, Myung-Hwan (Department of Biological Science Konkuk University)
  • Published : 2001.04.01
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Abstract

A monoclonal antibody (mAb) to the glucoprotein D (gD) of Herpes simplex virus type 2 (HSV-2) was successfully generated by hybridoma technology and characterized. The mAb, SKS2v, recognized a gD antigen with an apparent molecular mass of 60kDa in a Western blot analysis. The isotype was determined by a sandwich ELISA to be IgG2a. HSV-2 exhibited major antigens of 36, 43, 46, 47, 60, 69, 81, 96, 109, 112, 159, and 227 kDa among 25 protein profiles in SDS-PAGE, and among these antigens, those of 60, 112, 125, and 227 kDa were immunodominant in a Western blot analysis using antisera, thereby indicating that they play a role in inducing neutralizing antibodies in HSV-2 infection. When reacted with Vero cells infected with HSV-1 and HSV-2 SKSv2 showed a reactivity to the surface of the infected cells, and a gD antigen of 60 kDa appeared to be expressed in both types of HSV.

Keywords

References

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