Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Estimation of Theoretical Yield for Ethanol Production from D-Xylose by Recombinant Saccharomyces cerevisiae Using Metabolic Pathway Synthesis Algorithm

  • Lee, Tae-Hee (Department of Food Science and Technology, Research Center for New Biomaterials in Agriculture, Seoul National University) ;
  • Kim, Min-Young (Department of Computer Engineering, Seoul National University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University) ;
  • Seo, Jin-Ho (Department of Food Science and Technology, Research Center for New Biomaterials in Agriculture, Seoul National University)
  • Published : 2001.06.01
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Abstract

The metabolic pathway synthesis algorithm was applied to estimate the maximum ethanol yield from xylose in a model recombinant Saccharomyces cerevisiae strain containing the genes involved in xylose metabolism. The stoichiometrically independent pathways were identified by constructing a biochemical reaction network for conversion of xylose to ethanol in the recombinant S. cerevisiae. Two independent pathways were obtained in xylose-assimilating recombinant S. cerevisiae as opposed to six independent pathways for conversion of glucose to ethanol. The maximum ethanol yield from xylose was estimated to be 0.46 g/g, which was lower than the known value of 0.51 g/g for glucose-fermenting and wild-type xylose-fermenting yeasts.

Keywords

References

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