Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Bioelectrochemical Denitrification by Pseudomonas sp. or Anaerobic Bacterial Consortium

  • Published : 2001.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

In a bacterial denitrification test with Pseudomonas sp. and anaerobic consortium, more nitrates and less substrate were consumed but less metabolic nitrite was produced under an anaerobic $H_2$ condition rather than under $N_2$ condition. In a bioelectrochemical denitrification test with the same organisms, the electrochemically reduced neutral red was confirmed to be a substitute electron donor and a reducing power like $H_2$. The biocatalytic activity of membrane-free bacterial extract, membrane fraction, and intact cell for bioelectrochemical denitrification was measured using cyclic voltammetry. When neutral red was used as an electron mediator, the electron transfer from electrode to electron acceptor (nitrate) via neutral red was not observed in the cyclic voltammogram with the membrane-free bacterial extract, but it was confirmed to gradually increase in proportion to the concentration of nitrate in that of the membrane fraction and the intact cell of Pseudomonas sp.

Keywords

References

  1. Tibtech v.11 Controlling environmental nitrogen through microbial metabolism Cole, J.
  2. Agric. Biol. Chem. v.43 Application of clcctroenergizing method to L-glutamic acid fermentation Hongo, M.;M. Iwahara
  3. Wat. Res. v.28 Externa. Carbon source addition as a means to control an activated sludge nutrient removal process Isaacs, S.;M. Henze;H. Soeberg;M. Jummel
  4. Arch. Microbiol. v.161 Purification and characterization of membrane-bound hydrogenase from Methanosarcina barkeri MS Kemner, J. M.;J. G. Zeikus
  5. J. Bacteriol. v.146 Influence of corrinoid antagonists on methanogen metabolism Kenealy, W.;J. G. Zeikus
  6. J. Microbiol. Biotechnol. v.9 Direct electrode reaction of Fe(Ⅲ)-reducing bacterium, Shewanella putrefaciens Kim, B. H.;H. J. Kim;M. S. Hyun;D. H. Park
  7. J. Microbiol. Biotechnol. v.2 Hydrogen metabolism in Clostridium acetobutylcum fermentation Kim, B. H.;J. G. Zeikus
  8. Microbial. Rev. v.46 Denitrification Knowles, R.
  9. Ph.D. Thesis, Korea University Reduction of benzothiophene by cytochrome C3 from Desulfovibrio desulfuricans M6 reduced by hydrogenase and by electrochemical method Park, D. H.
  10. Appl. Environ. Microbiol. v.65 Microbial utilization of electrically reduced neutral red as the sole electron donor for growth and metabolite production Park, D. H.;M. Laivenieks;M. V. Guettler;M. K. Jain;J. G. Zeikus
  11. J. Bacteriol. v.181 Utilization of electrically reduced neural red by Actinobacillus succinogenes. Physiological function of neutral red in membrane-driven fumarate reduction and energy conservation Park, D. H.;J. G. Zeikus
  12. Appl. Environ. Microbiol. v.66 Electricity generation in microbial fuel cells using neutral red as an electronophore Park, D. H.;J. G. Zeikus
  13. J. Microbiol. Biotechnol. v.10 Denitrification characteristics and microorganism composition of acclimated denitrifier consortium Park, E. J.;J. K. Seo;J. K. Kim;K. H. Suh;S. K. Kim
  14. J. Bacteriol v.178 Involvement of the GroE chaperonins in the nickel-dependent anaerobic biosynthesis of NiFe-hydrogenases of Escherichia coli Rodrigur, A.;N. Batia;M. Muller;O. Fayet;R. Bohm;M. A. Mandrand-Berthelot;L. F. Wu
  15. Biochemistry v.32 Reversible electrochemistry of fumarate reductase immobilized on an electrode surface. Direct voltammetric observation of redox centers and their participation in rapid catalytic electron transport Sucheta, A.;R. Cammack;J. M. Weiner;F. A. Armstrong
  16. Bioelectrochem. Bioenerg. v.33 Tetracyanoquinonedimethane (TCNQ) modified electrode for NADH oxidation Surya, A.;N. Murthy;S. Anita
  17. Biology of Microorganisms Ecology of denitrification and dissimilatory nitrate reduction to ammonium Teidje, J. M.;Zehnder, A. J. E.(ed.)
  18. Biological Rev. v.41 Energy conservation in chemolithotrophic anaerobic bacteria Thauer, R. F.;K. Jungermann;K. Decker
  19. Wat. Res. v.17 Denitrification with methanol: Fundamental study of the growth and denitrification capacity of Hyphomicrobium sp. Timmermans, P.;A. Van Haute
  20. J. Bacteriol. v.181 Prokaryotic nitrate reduction: Molecular properties and functional distinction among bacterial nitrate reductase Vivian, C. M.;P. Cabello;M. N. Luque;R. Blasco;F. Castillo
  21. FEMS Microbiol. Lett. v.43 Clostridium thermoaceticum production of methanol from carbon monoxide in the presence of viologen dyes White, H.;H. Lebertz;I. Thanos;H. Simon
  22. Bioelectrochem. Bioenerg. v.29 Bioelectrocatalysed reduction of nitrate utilizing polythiophene bipyridium enzyme electrodes Willner, I.;E. Katz;N. Lapidot
  23. Arch. Microbiol. v.159 Energetics and regulation of formate and hydrogen metabolism by Metanobacterium formicicum Wu, W. M.;R. F. Hicky;M. K. Jain;J. G. Zeikus