Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Physical Analysis of nahQ tnpA Genes from Pseudomonas fluorescens

  • Seol, Ja-Young (Department of Life Science, Sookmyung Womens University) ;
  • Chol, Soon-Young (Division of Molecular Bilogy, Ewha Womens Univesrity) ;
  • Min, Kyung-Hee (Department of Life Science, Sookmyung Womens University)
  • Published : 2001.12.01
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Abstract

Pseudomonas fluorescens SM11 is a naphthalene-degrading strain whose dissimilatory genes are cho-mosomally encoded. We have cloned the 2.9 kb Sal I fragment harboring genes for the naphthalene-degrading upper pathway. The nucleotide sequences were determined to be nahQ, napA, and partial regions of nahE genes. The nahQ encods a protein of 188 amino acid residues with a deduced molec-ular wight of 20.8kDa. The high homology with other proteins suggests that NAhQ may be an active and useful protein whtich gives as selective advantage to naphthalene degradatin. Transposase(TnpA)encodes a polypetide chain with a molecular mass of 41.8kDa consisting of 376 amino acid residues. The deduced anino acid sequence of tnpA revealed 96% idenitity with putative transposase of P. stutzeri OX1,. It was assumed that transposase plays an important role in the evloution of the catabloic-path way in the regulation of nah expression.

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