Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimutagenic and Quinone Reductase Inducing Activities of Hericium erinaceus Extracts

노루궁뎅이 버섯 추출물의 항돌연변이원성 및 Quinone Reductase유도 효파

  • 박선희 (영남대학교 식품영양학과) ;
  • 김옥미 (영남대학교 식품영양학과) ;
  • 이갑랑 (대경대학 호텔쿠르리과)
  • Published : 2001.12.01
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Abstract

The effect of Herricium erinaceus on the mutagenicity in salmonella assay and quinone reductase activity in hapalclc7 cells were studied. Antimutagenic as evaluated by Ames test, the extract and fractions of JHerricium erinaceus had no effects on the mutagenicity by themselves. However, methanol extract and fractions from Hericium erinaceus showed strong inhibitory effect on the mutagenesis induced by N-methyl -N'-nitor-N- nitroso-guanidine (MNNG) and benzo(a)pyrene (B(a)P). Among the solvent fractions of methanol extract, the hexane fraction, the chloroform fraction and the ethylacetate fraction exhibited stronger inhibitory activity against MNNG and B(a)P induced mutagenesis than butanol and water fractions. The methanol extract, the extract, the chloroform and the ethylacetate fractions of Hericium erinaceus induced the activity of quinone reductase, an anticarcinogenic marker enzyme, in murine hepalclc7 cells while the others did have little effect on the enzyme activity.

본 연구는 옛부터 식용 및 약용으로 이용되고 있는 노루궁뎅이 버섯의 항돌연변이 및 암 예방 효과를 검색하기 위하여, 노루궁뎅이 버섯의 항돌연변이원성 및 quinone reductase (QR) 유도활성 효과를 살펴보았다. 노루궁뎅이 버섯의 돌연 변이 억제 효과를 Ames test로 검색한 결과, 노루궁뎅이 버섯의 메탄을 추출물과 분획물 그 자체의 돌연변이 원성은 없었으며, 노루궁뎅이 버섯의 메탄을 추출물은 직접변이원인 N- methyl- N'-nitro-guanidine(MNNG)와 간접변 이원인 B(a)P의 돌연변이 유발성을 크게 저해하였다. 또한 메탄올 추출물에서 분리한 분획물들도 유의성 있는 돌연변이 억제효과를 나타내었으며 특히 헥산, 클로르포름 및 에틸아세테이트 분획물의 경우는 부탄올과 물 분획 물보다 MNNG 와 13(a)P에 대해서 더 큰 항돌연변이 효과를 나타내었다. 그리고 노루궁뎅이 버섯에서 암 예방 효소계인 QR의 유도활성을 hepalclc7 세포주를 사용하여 검토한 결과에서는 노루 궁뎅이 버섯의 메탄을 추출묵과 헥산, 클로르포름 그리고 에틸아세테이트 분획물이 QR 효소활성을 유의적으로 증가시키는 것으로 나타났으며 나머시 분획물은 거의 효소활성에 영향을 미치지 않았다. 이상의 실험 결과로 노루궁뎅이 버섯은 발암물질로부터 생계를 보호하는 불질을 함유하고 있을 것으로 사료된다.

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