Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Effect of Quercetin on Melanogenesis in Melan-a Melanocyte Cells

Quercetin이 Melan-a 멜라닌세포의 멜라닌합성에 미치는 영향

  • Choi, Won-Hyung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University) ;
  • Chun, Hyun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine Wonkwang University)
  • 최원형 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 백승화 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 천현자 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2002.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Flavonoid seems to have various biological effects. Quercetin is a kind of natural plant flavonoids and has multiple biological effects such as antioxidant, antimutagenic and anticarcinogenec agent. Melanogenesis is a physiological process resulting in the synthesis of melanin pigments, which play a crucial protective role against skin photocarcinogenesis. This present study was designed to investigate effect of quercetin on proliferation and melanogenesis in Melan-a melanocyte cells. After 48h treatment of cells with quercetin, the cells exhibited a dose-dependent inhibition in their proliferation without apoptosis. Therefore, thε growth retardation by the extract may be due to the cell arrest or cell differentiation. We also investigated the effect of quercetin on melanogenesis of this cells. Melan-a melanocyte cells were grown for 48h in the presence of $0.01-60\;{\mu}g/ml$ quercetin and the total melanin content and activity of tyrosinase were measured. Quercetin stimulated melanization of the cells in low concentrations $(0.01-1.0\;{\mu}g/ml)$, whereas it inhibited melanization in high concentrations $(5.0-30\;{\mu}g/ml)$. It was observed that quercetin differently regulates melanogenesis of Melan-a melanocyte cells dependent on Its concentrations.

Keywords

References

  1. Pietta, P. G. (2000) Flavonoid as antioxidants. J. Nat. Prod. 63(7): 1035-1042 https://doi.org/10.1021/np9904509
  2. Park, J .C. and Kim, S. H. (1995) Flavonoid analysis from the leaves of eucommia ulmoides. J. Kor. Sco. Food Nutr. 24(6): 901-905
  3. Mun, S. I., Ryu, H. S., Lee, H. J. and Choi, J. S. (1994) Further screening for antioxidant activity of vegetable plants and its active principles from Zanthoxylum schinifotium. J. Kor. Sco. Food Nutr. 23(3): 466-471
  4. Kim, Y S., Song, K. S. and Cheong, H. S. (1996) Effects of flavonoids on pollen tube growth in arabidopsis thaliana. J. Plant biol. 39(4): 273-278
  5. Sorata, Y, Takahama, U. and Kimura, M. (1994) Protecdve effect of quercetin and rutin on photosentized lysis of human erocytes in the presence hematoporphyrin. Biochem. Biophys. Acta, 799: 313-316
  6. McClure, J. W. (1975) Physiology and function of flavo-noids. In 'Harbone, J. B., The flavoids'. Chapman and Hall, London 970
  7. Veckenstedt, A. and Pusztai, R. (1981) Investigation of the antiviral mechanism of quercetm against cardiovirus infec-don in mice. Antiviral Res. 1: 249-254 https://doi.org/10.1016/0166-3542(81)90015-2
  8. Hladovec, J. (1977) Antithrombotic effects of some flavo-noids alone and combined with acetylsalicylic acid, Arzneim, Forsch. 27: 1989
  9. Jones, E. and Hughes, R. E. (1993) Quercetin, flavonoids and the life span of mice, Exp. Gerontol., 117: 213-217
  10. Lee, C. J., Kim, H. D., Choung, E. H., Suh, J. K., Park, C. W. and Ha, J. S. (2000) Reduction effect of carcinogen- induced mouse epidermal and forestomach carcinogenesis by the extract of onion wastes. J. Kor. Soc. Food Sci. Nutr. 29(3): 525-530
  11. Kim, G. J, Oh, I. S., Hwang, W. K. and Kim, I. H. (1995) Phenolic compounds from cercis chinensis leaves. J. Pharm. Soc. Kor. 39(6): 600-609
  12. Park, J. C., Kim, B. W. and Young, H. S. (1990) Further Study on the flavonoids from the leaves of Machitus thunbergii in Korea, Kor. J. Pharmacogn. 21:197-201
  13. Kang, S. G., Kim, Y. D., Hyun, G. H., Kim, Y. H. Song, B. H., Sin, S. C. and Park, Y. G. (1998) Development of seperating techniques on quercetm-related substances in onion (Allium cepa L.)1. J. Kor. Soc. Food Sci. Nutr. 27(4): 682-686
  14. Kang, S. G., Kim, Y. D., Hyun, G. H., Kim, Y. H. Song, B. H., Sin, S. C. and Park, Y.G. (1998) Development of seperadng techniques on quercetin-related substances in onion (Allium cepa L.)2. J. Kor. Soc. Food Sci. Nutr. 27(4): 687-692
  15. Park, S. N., Choi, S. W. and Boo, Y. C. (1990) Effects of flavonoids of ginseng leaves on erythrocyte membranes against singlet oxygen caused damage. Kor. J. Ginseng Sci. 14(2): 191-199
  16. Moon, H .I., Ahn, K. T., Lee, K. R. and Zee, O. P .(2000) Flavonoid compounds and biological activities on the aehal parts of angelica gigas. J. Pharm. Soc. Kor. 44(2): 119-127
  17. Lichtenstein, L. M. (1977) Mediator release and asthma in ' asthma physiology, immunopharmacology and treatment'. editor Lichtenstein, L.M. and Austen K.F. Austen New york, Academic Press 93
  18. Park, J. C, Young, H. S., Yu, Y. B. and Lee, J. H. (1993) Studies on the chemical components and biological acdvites of edible plants in korea(I) : Phenolic compounds from the leaves of cedrela smensis A. Juss. J. Pharm. Soc. Kor. 37(3): 306-310
  19. Umeo, T. (1983) Suppression of lipid peroxidation by quercetin and its glycosides in spinach chloroplasts. Phytochem. Photobiol. 38: 363 https://doi.org/10.1111/j.1751-1097.1983.tb02684.x
  20. Hodnick, W. F, Kung, F. S., Roettger, W. L., Bohmont, C. W. and Pardini, R. S. (1986) Inhibition of mitochondnal respiration and production of toxic oxygen radicals by flavonoids. Biochem. Phamacol. 35: 2345-2357 https://doi.org/10.1016/0006-2952(86)90461-2
  21. Younes, M. and Siegers, C. P. (1981) Inhibitory action of some flavonoids on enhanced spontaneous lipid peroxidadon fellowing glutathione depledon. Ptanta Medica 43: 240 https://doi.org/10.1055/s-2007-971503
  22. Markaveiich, B. M., Roberts, R. R., Alejandio, M. A., Johnan, G. A., Middleditch, B. S. and dark, J. M. (1998) Bioflavonoid interaction with rat uterine type Ⅱ binding sites and growth inhibition. J. Steroid Biochemistry, 30: 71-78
  23. Tauber, A. L., Fay, J. R. and Marletta, M. A. (1984) Flavo-noid inhibition of the human neurophil NADPH oxidase. Biochem. Pharmacol. 33: 1367-1369 https://doi.org/10.1016/0006-2952(84)90194-1
  24. Warren, D. B., Louis, W. H. and Robert, W. W. (1987) The bioflavonoid quercedn inhibits neurophil degranuladon, superoxide production and phosphoryladon of specific neutrophil protein. Eiochem. Biophysic. Res. 144: 1229 https://doi.org/10.1016/0006-291X(87)91442-2
  25. Chen Q. X. and Kubo I. (2002) Kinetics of mushroom tyrosinase inhibition by quercetin. J. Agric Food Chem. 50(14): 4108-4112 https://doi.org/10.1021/jf011378z
  26. Yang M. J., Kim M. G., Lim S., Ann H. S. and Ahn R. M. (1999) Inhibitory effects of water-acetone extracts of chestnut inner shell, pine needle and hop on the melanin biosynthesis. J. Pharm. Soc. Kor. 43: 494-501
  27. Chun, H. J., Hwang, S. G., Lee, J.S., Baek, S. H., Jeon, B. H. and Woo, W. H. (2002) Inhibitory etfects of butyl alcohol extract from caesatpinia sappan L. on melanogenesis in melan-a cells. Kor. J. Phannacogn. 33(2): 1-7
  28. Chun, H. J., Choi, E. Y, Yoon, S. C., Nam, H. W. and Woo, W. H. (2001) Inhibitory effects of atractylodies rhizoma alba on melanin biosynthesis J. Pharm. Soc. Kor. 45(3): 269-275
  29. Chun, H. J., Hwang, S. G., Jeong, D. H., Baek, S. H., Jeon, B. H. and Woo, W. H. (2002) Butyl Alcohol extract from caesalpinia sappan L. regulates melanogenesis in B16 /F10 melanoma cells. J. Pharm. Soc. Kor. 46(2): 137-142
  30. Chun, H. J., Hwang, S. G., Kim, C. K., Jeon, B. H., Baek, S. H., and Woo, W. H. (2002) : hi vitro Modulation of proliferation and melanizadon of B16/F10 Melanoma cells by quercetin. J. Pharm. Soc. Kor. 46(1): 75-80
  31. Mosmann T. (1983) Rapid colorimetric assay for the cellular growth and survival : application to proliferation and cyto-toxic assay. J. Immun. Methods 65: 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  32. Hosoi J., Abe E., Suda T. and Kuroki T. (1985) Reguladon of melanin synthesis of B16 mouse melanoma cells by 1 a-25- dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45: 1474-1478
  33. Matinez-Esparza M. (1998) Mechanisms and melanogenesis inhibition by tumor necrosis factor-a in B16/F10 mouse melanoma cells. Eur. J. Biochem. 225: 139-146
  34. Vaux, D. L. (1993) Toward an understanding of the molecular mechanism of physiological cell death. Proc. Natl. Acad. Sci. USA 90: 786-789 https://doi.org/10.1073/pnas.90.3.786
  35. Hearing V. J. (1999) Biochemical control of melanogenesis and melanosomal organization. Soc. Invest. Dermatol. 4(1): 24-28 https://doi.org/10.1038/sj.jidsp.5640176
  36. Jimbow, K., Quevedo, W. C., Fitzpatnc, T. B., et al. (1993) Biology of melanocyte In: Fitzpatric, T. B., Eisen, A. Z., Wolff, K., et al. eds. Dermatology in general medicine, 4th ed. New York : McGraw-Hill Book, 261-289
  37. Bell, A. A. and Weeler, M. H. (1986) Biosynthesis and function of fungal melanin. Ann. Rev. Phytophathol. 24: 411-451 https://doi.org/10.1146/annurev.py.24.090186.002211
  38. Weixiong, L. and Helene, Z. H. (1997) Induced melanin reduces mutations and cell killing in mouse melanoma. Phytochem. Phytobiol. 65: 480-484 https://doi.org/10.1111/j.1751-1097.1997.tb08594.x
  39. Yaar, M. and Gilchiest B. A. (1991) Human melanocyte growth and differentiation : A decade of new data. J. Invest. Dermatol. 97: 611-615 https://doi.org/10.1111/1523-1747.ep12482985