Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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Effect of Indongcho(L. japonica Thunb) on Glucose and Lipid metabolism and Antioxidative Enzyme System in Streptozotocin-Induced Diabetic Rats

당뇨 유발쥐에서 인동초의 섭취가 혈청지질과 혈당 및 항산화효소계에 미치는 영향

  • Bang, Mi-Ae (Department of Food & Nutrition, Mokpo National University) ;
  • Kim, Hyeon-A (Department of Food & Nutrition, Mokpo National University) ;
  • Cho, Young-Ja (Department of Food & Nutrition, Mokpo National University)
  • 방미애 (목포대학교 생활과학대학 식품영양학과) ;
  • 김현아 (목포대학교 생활과학대학 식품영양학과) ;
  • 조영자 (목포대학교 생활과학대학 식품영양학과)
  • Published : 2002.10.31
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Abstract

The purpose of this study was to investigate the effects of dietary Indongcho(L. japonica Thunb) powder on blood glucose, serum lipid levels and antioxidative enzymes in normal and streptozotocin(STZ)-induced diabetic rats. Four groups of rats(3-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(NC), diabetic rats fed control diet(DC), normal rats fed Indongcho powder diet(NI), and diabetic rats fed Indongcho powder diet(DI). Diabetes was induced by single injection of streptozotocin(60mg/kg B.W., i.p.). The animals were fed ad libium each of the experimental diet for 5 weeks. Food and water intakes were determined everyday. Blood glucose and serum total cholesterol levels were determined every week. After 5 weeks the animals were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver and kidney homogenates. We also determined serum concentrations of total lipid(TL), total cholesterol(TC), triglycerides(TG) and HDL-cholesterol(HDL-C). Blood sugar and water intake were higher in diabetic group(DC and DI group) than normal group(NC and NI group) and were not significantly decreased by dietary Indongcho intake. Body weight gain and FER(feed efficiency ratio) were reduced by STZ treatment. But, Final body weight was recovered by Indongcho-contained diet. LHR(LDL-cholesterol/HDL-cholesterol) of the DI g re up was significantly lower than the other experimental groups(NC, NI and DC groups). The hepatic glucose 6-phosphatase(G6Pase) activity of the groups fed Indongcho diet(NI and DI group) was lower than the groups fed control diet(NC and DC group) and the G6Pase activity of NI group was recovered to the normal levels(p<0.05). However, The glutathione peroxidase(GPx) and glutathione reductase(GR) activities in liver and G6Pase activity in kidney were not statistically different between the control and diabetic control groups. Renal GST activity of the DI group was recovered by Indongcho intake. In conclusion, these results confirm oxidative stress in the liver and kidney of rats with STZ diabetes and antioxidative effect of Indongcho.

Keywords

References

  1. 백희영, 김교정, 김영옥, 김정순, 문현경, 오세영, 이순영, 이심열, 정효지, Dwyer J., 한국인의 건강영양조사의 필요성 in: 한국인의 건강영양조사, 서울대학교 출판부, 1997
  2. Stevens MJ, Feldman EL, Greene DA. The etiology of diabetic neuropathy: The combined roles of metabolic and vascular defects. Diabetic Med 12:1566-579, 1995
  3. Reaven GM. Role of insulin resistance in human disease. Diabetes 37:1595-1607, 1988 https://doi.org/10.2337/diabetes.37.12.1595
  4. Vitamins, 1997년 겨울호, 한국비타민 정보 센터, 1997
  5. Adeghate E, Parvez SH. Nitric oxide and neuronal and pancreatic beta cell death. Toxicology 16; 153(1-3): 143-56, 2000 https://doi.org/10.1016/S0300-483X(00)00310-3
  6. Laybutt DR, Kaneto H, Hasenkamp W, Grey S, Jonas JC, Sgroi DC, Groff A, Ferran C, Bonner-Weir S, Sharma A, Weir GC. Increased Expression of Antioxidant and Antiapoptotic Genes in Islets That May Conthbute to [beta]-Cell Survival During Chronic Hyperglycemia. Diabetes Feb; 51(2): 413-423, 2002 https://doi.org/10.2337/diabetes.51.2.413
  7. Reddi AS. Riboflavin nutritional status and flavoprotein enzymes in streptozotocin-diabetic rats. Biochim Biophys Acta 3; 882(1): 71-6, 1986 https://doi.org/10.1016/0304-4165(86)90057-7
  8. Melo SS, Arantes MR, Meirelles MS, Jordao AA Jr, Vannucchi H., Lipid peroxidation in nicotinamide-deficient and nicotinamide-supplemented rats with streptozotocin-mduced diabetes. Acta Diabetol Mar; 37(1): 33-9, 2000 https://doi.org/10.1007/s005920070033
  9. 정진섭, 김재가: 원색천연약물대사전 상권 p112남산당(1984)
  10. 한국생약학교수협의회 본초학(1995), 207-209 사단법인 대학약사회., 서울
  11. Baginski E.S., Foa P.P. and Zak B., Glucose 6-phosphatase In Methods of Enzymematic Analysis Vol. 2. Academic Press, New York, pp 876-880, 1983
  12. Buege J.A. and Aust S.D.'In Sidney F. and Lester P.(eds.), Methods in Enzymology Vol. 52, Academic Press, New York, pp302-310, 1978
  13. Habig W.H., Pabst M.J. and Jakoby W.B., Glutathione S-transferase,J.Biol. Chem. 249:7130-7139, 1974
  14. Tappel AL Glutathione peroxidase and hydroperoxides, In Methods in enzymology(Fleisher S & Packer Led) 52:506-513, 1978
  15. Carlberg I. and Mannervick B., Glutathione reductase, In Methods in enzymology(Fleisher S & Packer Led) 113:484-499, 1985
  16. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.T., Protein measurement with the folin phenol reagent, J. Biol. Chem. 193:265-275, 1951
  17. Goldbergy RB, Lipid disorders in diabetes, Diabetes Care 4:561-572, 1981 https://doi.org/10.2337/diacare.4.5.561
  18. West KM, Ahuja MMS, Beimett PH, et al. The role of circulating glucose and thglycende concentration and their interaction with other "risk factor" as determinants of arterial disease in nine diabetic population samples from WHO multinational study. Diabetes Care 6:361-369, 1983 https://doi.org/10.2337/diacare.6.4.361
  19. ChoSY,ParkJY,ParkEM,ChoiMS,LeeMKJeonSM,JangMK, Kim MJ, Park YB. Altemation of hepatic antioxidant enzyme activities and lipid profile in streptozotodn-induced diabetic rats by supplementation of Dandelion water extract. Clin Chim Acta 317(1-2): 109-17, 2002 https://doi.org/10.1016/S0009-8981(01)00762-8
  20. Ghosh R, Mukherjee B, Chatterjee M. A novel effect of selenium on streptozotocin-induced diabetic mice. Diabetes Res 25(4): 165-71, 1994
  21. Argaud, D., Zhang, Q., Pan, W., Maitra, S., Pilkis, S. J., and Lange, A. J. Diabetes 45,1563-1571, 1996 https://doi.org/10.2337/diabetes.45.11.1563
  22. Tiinh, K. Y., O' Doherty, R. M., Andeison, R, Lange, A. J., and Newgard, C. B. Perturbation of Fuel Homeostasis Caused by Overexpression of the Glucose-6-phosphatase Catalytic Subunit in Livei of Normal Rats J. Biol. Chem. 273,31615-31620, 1998 https://doi.org/10.1074/jbc.273.47.31615
  23. Liu Z, Barrtt EJ, Dalkin AC, Zwart AD, Chou JY Effect of acute diabetes on the rat hepatic glucose-6-phosphatase activity and its messenger RNA level. Biochem Biophy Res Conunun 30; 205(1) 680-686, 1994 https://doi.org/10.1006/bbrc.1994.2719
  24. Kedziora-Kornatowska K, Luciak M. Effect of aminoguanidine on lipid peroxidation and activities of antioxidant enzymes in the diabetic kidney iochem Mol Biol Int Oct; 46(3): 577-83, 1998
  25. Celik S, Baydas G, Yilmaz O. Influence of vitamin E on the levels of fatty acids and MDA in some tissues of diabetic rats. Cell Biochem Funct. 20(1): 67-71, 2002 https://doi.org/10.1002/cbf.936
  26. Kinalski M, Sledziewski A, Telejko B, Zarzycki W, Kinalska I.Lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes. Acta Diabetol 37(4): 179-8, 2000 https://doi.org/10.1007/s005920070002
  27. Rouer E, Mahu JL, Dansette P, Leroux JP. UDP-glucuronosyltransferase, epoxide hydrolase and glutathione S-transferase activities in the liver of diabetic mice. Biochim Biophys Acta 676(2): 274-7, 1981 https://doi.org/10.1016/0304-4165(81)90197-5
  28. Agius C, Gidari AS. Effect of streptozotocin on the glutathione S-transferases of mouse liver cytosol. Biochem Pharmacol 15; 34(6): 811-9, 1985 https://doi.org/10.1016/0006-2952(85)90761-0
  29. Ramanathan M, Jaiswal AK, Bhattacharya SK. Superoxide dismutase, catalase and glutathione peroxidase activities in the brain of streptozotocin induced diabetic rats. Indian J Exp Biol 37(2): 182-3, 1999
  30. Hermenegildo C, Raya A, Roma J, Romero FJ. Decreased glutathione peroxidase activity in sdatic nerve of alloxan-induced diabetic mice and its correlation with blood glucose levels. Neurochem Res. 18(8): 893-6, 1993 https://doi.org/10.1007/BF00998274
  31. Wobaieb SA, Godin DV. Alterations in tissue antioxidant systems in the spontaneously diabetic (BBWistar) rat. Can J Physiol Pharmacol 1987 Nov; 65(11):2191-5, 1987 https://doi.org/10.1139/y87-346