Flavor Improvement of Chungkookjang by Addition of Yucca (Yucca shidigera) Extract

유카(Yucca shidigera) 추출물 첨가에 의한 청국장의 풍미 개선에 관한 연구

  • Published : 2002.02.01

Abstract

This study was carried out to investigate the effect of yucca extract on the flavor development in Chungkookjang fermented by Bacrillus sp. b01. The changes in the contents of amino-type N, ammonia type N, volatile compounds and organic acids, and those in the activities of ${\alpha}-amylase$ and protease were also determined with aging period. The amount of amino-type N increased gradually with time and was slightly higher in Chungkookjang containing yucca extract than in control. The content of ammonia-type N in Chungkookjang decreased by the addition of yucca extract. During aging, a little higher amylase activity was detected in Chungkookjang containing yucca extract. However, the amylase activity was the lowest in Chungkookjang containing 1 mg/g of yucca extract. The protease activity was slightly higher in Chungkookjang containing yucca extract. The organic acids, such as citrate, acetate, malate, fumarate, and succinate, were detected. The yucca extract reduced the production of tetramethylpyrazine which was responsible for the unpleasant odor, but enhanced the production of 2,5-dimethylpyrazine and 2,6-dimethylpyrazine which contributed to the taste. Sensory evaluation showed that the addition of yucca extract of 0.5 mg/g significantly improved the flavor and taste of products.

Bacrillus sp. b01 균주를 사용한 청국장 제조 시 yucca 추출물의 첨가가 청국장의 숙성에 미치는 영향을 검토하고자 청국장 숙성 과정중의 아미노태 질소, 암모니아태 질소, amylase 활성, protease 활성, 유기산 및 향기성분의 변화를 조사하였다. 청국장 숙성 중 yucca 첨가구에서 아미노태 질소 함량은 증가하였으며, 암모니아태 질소는 감소하였다. Amylase 활성은 yucca 첨가구들이 대조구와 비교 시 높게 나타났지만 yucca 추출물을 1 mg/g 첨가 시 감소함을 보였다. Protease 활성은 yucca 추출물 첨가구가 무 첨가구보다 다소 높게 나타났다. 청국장에서의 유기산을 citric acid, acetic acid, malic acid가 검출되었고, yucca 첨가구에서 대조구에 비하여 그 함량이 다소 높았다. 또한 yucca 추출물 첨가 시 2,5-dimethylpyrazine과 2,6-dimethylpyrazine은 증가하였고, tetramethylpyrazine이 숙성 기간 중 감소함을 보였다. 관능검사에서도 yucca 추출물 0.5 mg/g을 첨가시 무 첨가구보다 유의적으로 맛과 향이 우수하였다.

Keywords

References

  1. Kim, K.J., Ryu, M.K. and Kim, S.S. Chungkookjang koji fermen-tation with rice straw. Korean J. Food Sci. Technol. 14: 301-308 (1982)
  2. Yoo, J.Y. Present status of industries and research activities of Korean fermented soybean products. The Microorganism and Industry23: 13-30 (1997)
  3. Her, S., Lee, S.K. and Joo, H.K. Isolation and identification of the fibrinolytic bacteria from Korean traditional Chungkookjang. J. Korean. Soc. Agric. Chem. Biotechnol. 41: 119-124 (1998)
  4. Joo, H.K. and Yun, S.E. Imprvement on flavor and taste of Chun-gkookjang by freeze denaturation of soybean. Daesan Nonchong 5: 161-174 (1997)
  5. Yoo, J.H. Effect of Artemista Nakai extract on the flavor of Chungkookjang. Master thesis, Konkuk University. (1996)
  6. Masazumi, M., Yukiyishi, T, Hitoshi, M., Kenji, M., Osamu, T., Takao, I., Kazuhiro, O., Ryoji, K. and Kazuo, Y. Antiyeast steroi-dal saponins from Yucca shidigera(Mohave Yucca), a new anti-food-detenorating agent. J. Nat. Prod. 63: 332-338 (2000) https://doi.org/10.1021/np9904354
  7. Segal, R. and Schlosser, E. Role of glycosidases in the membran-lytic, antifungal action of saponins. Arch. Microbiol. 104, 147-150 (1975) https://doi.org/10.1007/BF00447315
  8. Wallace, R.J., Arthaud, L. and Newbold, C.J. Influence of Yucca shidigera extract on ruminal ammonia concentration and ruminal microoranisms. Appl. Environ. Microbiol. 60: 1762-1767 (1994)
  9. Robert B., Harris, D.H. and Tom, L. Yucca plant saponin in the treatment hypertention and hypercholesterolemia. J. Appl. Nutr. 30:374-381 (1978)
  10. Yeo, J. and Kim, K.I. Effect of teeding diets containing an antibi-otic, a probiotic or yucca extract on growth and intestinal urease activity in broiler chicks. Poultry Sci. 76: 381-385 (1997) https://doi.org/10.1093/ps/76.2.381
  11. Weatherburn, M.W. Phenol-Hypochorite reaction for determination of ammonia. J. Anal. Chem. 39: 971-974 (1967) https://doi.org/10.1021/ac60252a045
  12. Yoon, K.S. Changes of enzymetic activities during the fermenta-tion of soybean. Master thesis. Konkuk University (1988)
  13. Anson, M.L. The estimation of pepsin, trypsin, Papain and cathe psin with hemoglobin. J. Gen. Physiol. 22: 79-85 (1939) https://doi.org/10.1085/jgp.22.1.79
  14. AOAC Official Methods of Analysis Association of Official Ana-lytical Chemists. Vol. 2, pp. 11-16 (1995)
  15. Lee, B.Y., Kim, D.M. and Kim, K.H. Studies on the change in rheological properties of Chungkookjang. Korean J. Food Sci. Technol. 23: 478-484 (1991)
  16. Choi, U.K., Ji, W.D. and Chung, Y.G. Characteristics of Chungk-ookjang produced by B. subtilis DC-2. J. Food Sci. Nutr. 27: 846-856 (1998)
  17. Suh, J.S., Lee, S.G. and Ryu, M.K. Effect of Bacillus strains on the Chungkookjang processing II. Change of the components and enzyme activities duhng the storage of Chungkookjang. Korean J. Food Sci. Technol. 14: 309-314 (1982)
  18. In, Z.P. Flavor enhancement of Chungkookjang by addition of yucca (Yucca shidigera) extract. Master thesis, Konkuk University (2000)
  19. Makkar, H.S., Sen, S., Blummel, M. and Becker, K. Effects of fraction containing saponins from Yucca shidigera, Quillaja saponaria and Acacia auriculoforms on rumen fermentation. J. Agri. Food Chem. 46: 4324-4328 (1998) https://doi.org/10.1021/jf980269q
  20. Wang, Y., Mcallister, T.A., Newbold, C.J., Rode, L.M., Cheeke PR. and Cheng, K.J. Effect of Yucca shidigera extract on fermen-tation and degradation of steroidal saponins in the rumen simula-tion technique(Rusitec). Anim. Feed Sci. Technol. 74: 143-153 (1998) https://doi.org/10.1016/S0377-8401(98)00137-0
  21. Valdez, F.R., Bush, L.J., Goetsch, A.L. and Owens, N. Effect of steroidal sapogenins on ruminal fermentation and on producdon of lactating dairy cows. J. Dairy Sci. 69: 1568-1575 (1986) https://doi.org/10.3168/jds.S0022-0302(86)80573-2
  22. Han H.S. A study on the change of chemical composition during the ripening process of fennented soybean with mugwort powder. Master thesis, Konkuk University (1994)
  23. Ko, T.G., Kim, J.D., Bae, S.H., Han, Y.K. and Han. I.K. Study for the development antibiotics-free diets for weanling pigs. Korean J. Anim. Sci. 42: 37-44 (2000)
  24. Bae, K.H., Ko, T.G., Kim, J.H., Cho, W.T., Han, Y.K. and Han, I.K. Use of metabolically active substance to substitute for antibi-otics in finishing pigs. Korean J. Anim. Sci. 41: 23-30 (1999)
  25. Sulistyo, J., Naotoshi, T., Kazumi, F. and Kan, K. Production of natto starter. Nippon Shokuchin Kogyo Gakkaishi, 35: 280-281 (1988)
  26. Son, D.H., Kwon, O.J., Ji, W.D., Choi, U.K., Kwon, O.J., Lee, E.J., Cho, Y.J., Cha, W.S. and Chung, Y.G. The quality of Chungkookjang prepared by Bacillus sp. CS-17 during fermentation time. J. Korean Soc. Agric. Chem. Biotechnol. 43: 1-6 (2000)
  27. Ji, W.D., Yang, S.H., Choi, M.R., and Kim, J.K. Volatile compo-nents Korean soybean paste produced by Bacillus subtilis PM3. J. Microbiol. Biotechnol. 5: 143-147 (1995)
  28. Sugahara, E., Ito, T, Odagiri, S., Kubota, K. and Kobayashi, A. Comparison of odor components of natto and cooked soybean. Agric. Biol. Chem. 49: 311-318 (1985) https://doi.org/10.1271/bbb1961.49.311
  29. Choi, S.H. and Ji, Y.A. Change in flavor of Chungkookjang dur-ing fermentation. Korean J. Food Sci. Technol. 21: 229-234 (1989)
  30. Choi, C., Choi, K.S., Choi, J.S., Lim, S.L., Lee, S.H., Son, J.H., Choi, H.J. and Lee, H.D. Characteristics and action pattern of protease from Bacillus subtilis globigii CCKS-118 in Korean tra-ditional soy sauce. Agric. Chem. Biotechnol. 39: 460-465 (1996)
  31. Kim, B.N., Park, C.H., Han, C.H., S.S., and Lee, S.Y. Flavor component, fatty acid and organic acid of natto with spice added. J. Korean. Soc. Food Nutr. 24: 219-227 (1995)