Preparation and Antioxidant Activity of Health Drink with Extract Powders from Safflower (Carthamus tinctorius L.) Seed

홍화씨 추출분말 함유 건강음료의 제조와 항산화성

  • Kim, Jun-Han (Department of Food Science and Technology, Kyungpook National University) ;
  • Park, Jun-Hong (Uisong Medicinal Plant Experiment Station, Kyungpook Provincial A.T.A.) ;
  • Park, So-Deuk (Uisong Medicinal Plant Experiment Station, Kyungpook Provincial A.T.A.) ;
  • Choi, Seoing-Yong (Uisong Medicinal Plant Experiment Station, Kyungpook Provincial A.T.A.) ;
  • Seong, Jong-Hwan (Department of Food Science, Miryang National University) ;
  • Moon, Kwang-Deog (Department of Food Science and Technology, Kyungpook National University)
  • 김준한 (경북대학교 식품공학과) ;
  • 박준홍 (경북농업기술원 의성약초시험장) ;
  • 박소득 (경북농업기술원 의성약초시험장) ;
  • 최성용 (경북농업기술원 의성약초시험장) ;
  • 성종환 (밀양대학교 식품과학과) ;
  • 문광덕 (경북대학교 식품공학과)
  • Published : 2002.08.01

Abstract

Health drinks were prepared with freeze dried powder of 60% ethanol extract (60% EFDP), 60% ethanol extract after hydrolysis with amyloglucosidase (60% AEFDP) and 80% ethanol extract (80% EFDP) from roasted safflower seed. Quality characteristics and antioxidative properties were investigated. Yield of freeze dried powders were ranged in $4.67%{\sim}5.62%$. Brix, pH and titratable acidity of safflower drinks were ranged in $11.4{\sim}14.2%$, $2.83{\sim}3.34$ and $0.09{\sim}0.91%$, respectively. Content of total phenolic compounds was much more in 80% EFDP (117 mg/g) and safflower drink-I (SD-I, 440 ppm) than others. Content of total flavonoid was observed in higher level in 60% EFDP (49 mg/g) and safflower drink-V (SD-V, 138 ppm) than others. Antioxidant compounds such as N-[2-(5-hydroxy-1H-indol-3-yl)ethyl]ferulamide(serotonin-I) and N-[2-(5-hydroxy-1H-indol-3yl)ethyl]-p-coumaramide(serotonin-II) exhibited higher contents of 21.09 ppm, 33.56 ppm in 60% EFDP and of 3.83 ppm, 5.81 ppm in safflower drink-II (SD-II) than others. Content of acacetin was much more in 80% EFDP (13.53 ppm) and safflower drink-IV (SD-IV, 1.14 ppm) than others. From the DPPH test to measure antioxidant activity, it was shown that 80% EFDP and SD-I have stronger scavenging activities of 94.58% and 94.88%, respectively, while BHA standard solution does 93.88%. Among drinks, SD-II was revealed to have highest level on overall acceptance, color and flavor through sensory evaluation. These results induced that safflower seed can be used as natural antioxidant and functional food material.

홍화씨 에탄올추출 동결건조분말을 이용한 건강음료의 제조와 그의 품질특성 및 항산화성을 조사하였다. 추출건조분말의 수율은 amyloglucosidase 가수분해 후 60% 에탄올추출물이 5.62%로 가장 높았다. 음료제품의 당도는 $11.4{\sim}14.2%$, pH는 $2.83{\sim}3.34$, 산도는 $0.09{\sim}0.91%$이였고, 총페놀 함량은 80% 에탄올추출 동결건조분말이 117 mg/g로, 음료제품은 SD-I이 440 ppm으로 가장 높았고, 총플라보노이드 함량은 60% 에탄올추출 동결건조분말이 49 mg/g로, 음료제품 SD-V가 138 ppm으로 가장 높았다. 또한, serotonin 화합물인 serotonin-I과 serotonin-II의 함량은 80% 에탄올추출 동결건조분말과 amyloglucosidase 가수분해 후 60% 에탄올 추출 동결건조분말에서 가장 높은 21.09 ppm과 33.56 ppm의 함량을 보였고, 홍화음료의 경우는 SD-II에서 3.83 ppm과 5.81 ppm으로 가장 높게 나타났으며, acacetin의 함량은 80% 에탄올추출 동결건조분말에서 가장 높은 13.53 ppm의 함량을 보였고, 홍화음료에서는 SD-V가 1.14 ppm으로 가장 높은 함량을 나타내었다. 또한, DPPH 소거활성은 80% 에탄올추출 동결건조분말이 94.51%로, 음료제품 SD-I이 94.88%을 나타내어 100 ppm BHA 표준용액의 93.88%보다 뛰어난 항산화 활성을 보였다. 홍화음료의 관능평가에서는 SD-I과 SD-II가 전제적 기호도, 색 및 향 등의 모든 관능평가 항목에서 전반적으로 높은 관능점수를 얻었다. 이러한 결과들은 항산화제 및 기능성식품의 소재로서의 홍화씨의 식품학적 가치를 높여 주는 역할을 할 수 있으리라 생각된다.

Keywords

References

  1. Kim, J.H., Jeon, S.M., An, M.Y., Ku, S.K., Lee, J.H., Choi, M.S. and Moon, K.D. Effects of diet of Korean safflower (Carthamus tinctorious L.) seed powder on bone tissue in rats during the recovery of rib fracture. J. Korean Soc. Food Sci. Nutr. 27: 698-704 (1998)
  2. Jeon, S.M., Kim, J.H., Lee, H.J., Lee, I.K., Moon, K.D. and Choi, M.S. The effects of Korean safflower (Carthamus tinctori-ous L.) seed powder supplementation diet on bone metabolism indices in rats during the recovery of rib fracture. Korean J. Nutr.31: 1049-1056(1998)
  3. Seo, H.J., Kim, J.H., Kwak, D.Y., Jeon, S.M., Ku, S.K., Lee, J.H., Moon, K.D. and Choi, M.S. The effects of safflower seed powder and its fraction on bone tissue in ribfractured rats during the recovery. Korean J. Nutr. 33: 411-420 (2000)
  4. Moon, K.D., Back, S.S., Kim, J.H., Jeon, S.M., Lee, M.K. and Choi, M.S. Safflower seed extract lowers plasma and hepatic lip-ids in rats fed high-cholesterol. Nutr. Res. 21: 895-904 (2001) https://doi.org/10.1016/S0271-5317(01)00293-7
  5. Nast, H.G., Katkhuda, N. and Tannir, I. Effects of fertilization and population rate-spacing on sadower yield and other charac-terisdcs. Agron. J. 70: 683-685 (1978) https://doi.org/10.2134/agronj1978.00021962007000040037x
  6. Kennedy, W.K. and Unrau, J. A rapid method for determining the oil content of safflower and sunflower seeds. Agron. J. 41: 93-95(1949) https://doi.org/10.2134/agronj1949.00021962004100020009x
  7. Yook, C.S. Korean Medical Plants, P. 241. Gyechuk Publishers,Seoul(1981)
  8. Zhang, H.L., Nagatsu, A., Watanabe, T, Sakakibara, J. and Okuyama, H. Antioxidative compounds isolated from safflower (Carthamus tinctorius L.) oil cake. Chem. Pharm. Bull. 45: 1910-1914(1997) https://doi.org/10.1248/cpb.45.1910
  9. Roh, J.S., Sun, W.S., Oh, S.U., Lee, J.I., Oh, W.T. and Kim, J.H. In vitro antioxidant activity of safflower (Carthamus tinctorius L.) seeds. Food Sci. Biotechnol. 8: 88-92 (1999)
  10. Kang, G.H. Antioxidative activity of phenolic compounds isolated from safflower (Carthamus tinctorius L.) seeds. M.S. thesis. Catholic University of Daegu, Daegu (2001)
  11. Kim, H.J., Jun, B.S., Kim, S.K., Cha, J.Y. and Cho, Y.S. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). J. Korean Soc. Food Sci. Nutr. 29: 1127-1132(2000)
  12. Gardner, C.R. and Walker, R.J. The roles of putative neurotrans-mitters and neuromodulators in annelids and related invertebrates.Prog. Neurobiol. 18: 1-120 (1982) https://doi.org/10.1016/0301-0082(82)90002-8
  13. Gerschenfeld, H.M., Paupardin-Tritsch, D. and Deterre, P. Neu-ronal responses to serotonin: a second view. pp. 105-130. In: Serotonin Neurotransmission and Behaviour. Jacobs, B.L. and Gelperin, A. (eds.). MIT Press, London, UK (1981)
  14. Kim, J.H., Choi, M.S. and Moon, K.D. Quality characteristics of bread prepared with the addition of roasted safflower seed pow-der. Korean J. Postharvest Sci. Technol. 7: 80-83 (2000)
  15. Choi, J.H., Jang, J.G., Park, M.H. and Oh, S.K. High perfor-mance liquid chromatographic determination of free sugars in ginseng and its products. Korean J. Pood Sci. Technol. 13: 106-107(1981)
  16. Oh, S.L., Kim, S.S., Min, B.Y. and Chung, D.H. Composition of free sugars, free amino acids, non-volatile organic acids and tan-nins in the extracts of L. chinensis M., A. acutiloba K., S. chin-ensis B. and A. sessiliflorum S. Korean J. Food Sci. Technol. 22:76-81 (1990)
  17. Lister, C.E., Lancaster, J.E. and Sutton, K.H. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar. J. Sci. Food Agric. 64:155-161 (1994) https://doi.org/10.1002/jsfa.2740640204
  18. Nielsen, S.E. and Dtagsted, L.O. Column-switching high- perfor-mance liquid chromatographic assay for determination of apigenin and acacetin in human unine with ultraviolet absorbance detec-tion. J. Chromatogr. 713: 379-386 (1998) https://doi.org/10.1016/S0378-4347(98)00187-X
  19. Wanasundara, U., Amarowicz, R. and Shahidi, F. Isolation and identification of an antioxidative component in canola. J. Agric. Food Chem. 42: 1285-1290 (1994) https://doi.org/10.1021/jf00042a006
  20. Nowak, K., Kujawa, R. and Zademowski, R. Antioxidative and antibacteral properties of phenolic compounds in rapeseed. Fat Sci. Technol. 94: 149-152 (1992)