Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Identification and Activity of Antioxidative Compounds from Rubus coreanum Fruit

복분자에 함유된 항산화물질의 동정 및 활성

  • Yoon, In (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Cho, Jeong-Yong (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Kuk, Ju-Hee (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Wee, Ji-Hyang (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Jang, Mi-Young (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Ahn, Tae-Hoe (Research Center, Ottogi Food Co., Ltd.) ;
  • Park, Keun-Hyung (Department of Food Science and Technology and Institute of Agricultural Science and Technology, Chonnam National University)
  • 윤인 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 조정용 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 국주희 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 위지향 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 장미영 (전남대학교 식품공학과 및 농업과학기술연구소) ;
  • 안태회 (오뚜기 식품 중앙연구소) ;
  • 박근형 (전남대학교 식품공학과 및 농업과학기술연구소)
  • Published : 2002.10.01
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Abstract

The ehtyl acetate-soluble acidic fractions from juice and cake of Rubrs coreanum fruit showed DPPH radical-scavenging activity. Each fraction was purified through silica gel adsorption column chromatography. The compounds in the ethyl acetate-soluble acidic fraction of juice were identified as 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, and 3,4-dihydroxycinnamic acid by GC-MS. The compounds in the ethyl acetate-soluble acidic fraction of cake were also identified as succinic acid, 3,4-dihydroxybenzoic acid, citric acid, 3,4,5,-trihydroxybenzoic acid, and 3,4-dihydroxycinnamic acid by GC-MS. Antioxidative activity of the identified compounds were evaluated by DPPH radical-scavenging assay and hydroxyl radical-scavenging activity assay.

복분자를 착즙하여 착즙액과 잔사로 나누고, 각 추출물을 용매분획하여 얻어진 각 획분을 대상으로 DPPH radical-scavenging 활성을 측정하였더니, 각 추출물의 EtOAc 가용산성획분에서 비교적 강한 활성이 나타났다. 이들 획분에 함유된 활성물질을 규명하고자 silica gel column chromatography로 정제한 다음 활성획분을 GC-MS 분석을 실시한 결과, 복분자 착즙액의 EtOAc 가용 산성획분에 함유된 물질로 4-hydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid, and 3,4-dihydroxycinnamic acid 등이 동정되었고, 잔사 MeOH 추출물의 EtOAc 가용 산성획분에 함유된 물질로 succinic acid, 3,4-dihydroxybenzoic acid, citric acid, 3,4,5-trihydroxybenzoic acid, 3,4-dihydroxycinnamic acid가 동정되었다. DPPH radical-scavenging 활성과 hydroxyl radical에 의한 malonaldehyde 생성억제 효과활성 등의 방법으로 동정된 물질의 항산화 활성이 평가되었다.

Keywords

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