Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Analysis of Genetic Relationship by RAPD Technique for Codonopsis lanceolata Trauty Collected from the Baekdoo Mountain and Korea

백두산지역과 국내 더덕 수집종의 RAPD에 의한 유연관계 분석

  • Doo, Hong-Soo (National Honam Agricultural Experiment Station) ;
  • Ryu, Jeom-Ho (Faculty of Biological Resources Science (The Institute of Agricultural Science & Technology), Jeonbuk National University) ;
  • Lee, Kang-Soo (Faculty of Biological Resources Science (The Institute of Agricultural Science & Technology), Jeonbuk National University) ;
  • Li, Hu Lin (Faculty of Agriculture, Yanbian University) ;
  • Liu, Xian Hu (Faculty of Agriculture, Yanbian University)
  • 두홍수 (호남농업시험장) ;
  • 류점호 (전북대학교 생물자원과학부(농업과학기술연구소)) ;
  • 이강수 (전북대학교 생물자원과학부(농업과학기술연구소)) ;
  • 이호림 (연변대학 농학원) ;
  • 유헌호 (연변대학 농학원)
  • Published : 2002.09.30
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Abstract

Extracted genomic DNA from 16 accessions of Codonopsis lanceolata collected from South Korea and the Baekdoo Mt. areas of China were analyzed for their genetic relationships by RAPD. Twenty 10-mer-oligonucleotide primers having reproductive polymorphism were selected for the RAPD analysis. The size of amplified DNA was almost between 125 bp and 2.0 kbp. Sixteen collected Codonopsis lanceolata were analyzed with 20 primers which generated 73(49.3%) polymorphic bands among 148 PCR products. The mean number of polymorphic bands were 7.4 and varied $1{\sim}9$ per primer. It was, thus, demonstrated that RAPD was useful for detecting polymorphism in Codonopsis lanceolata. The range of 1-F value(genetic similarity) was from 0.682 to 0.959. These results indicate variable genetic similarities. By UPGMA (Unweighted Pair Group Method using an Arithmetic average) cluster analysis based on 1-F value, genetic distance among the 16 collected Codonopsis lanceolata was $0.133{\sim}0.400$. It was certainly classified into two groups between collected accessions from Korea and China, and the genetic distance was about 0.281. Both accessions collected from Korea and China showed miner differences, while the genetic relationships of Tonghua Xian and Liuhe Xian from China was farthest with other accessions collected.

한국과 중국 동북부 지역에서 수집한 16종의 더덕으로부터 genomic DNA를 추출한 후, Bioneer사로부터 구입한 random primer(10-mer)를 이용하여 PCR을 수행하였다. 총 49종의 primer를 스크린 한 결과 재현성이 있으면서 polymorphism을 보이는 20개의 primer를 선발하였다. 선발한 primer로부터 PCR에 의해 증폭된 DNA의 크기는 125 bp에서 2.0 kb 내외였으며, 총 148개의 band가 관찰되어 평균 band 수는 7.4개였다. 이들 중에서 polymorphism을 보이는 band의 수는 73개이었으며 (49.3%), polymorphism은 각 primer별로 $1{\sim}9$개로써 다양하였다. 16개 수집종의 유사계수 범위는 0.682에서 0.959로써 유전적 유연정도는 크지 않았다. UPGMA 분석에 의한 수집종들의 유연관계를 dendrogram으로 나타낸 바, 수집종들간의 유전적 거리는 $0.133{\sim}0.400$이었으며, 국내 수집종과 중국 수집종간에는 확실하게 두 그룹으로 분류되었고, 유전적 거리는 약 0.281이었으며, 이들은 모두 지역적인 차이를 보였다. 한편, 중국의 '통화현(通化縣)'과 '류하현(柳河縣)' 수집종이 다른 지역의 수집종보다 유전적 거리가 가장 크게 나타났다.

Keywords

References

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