Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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AN HISTOPATHOLOGICAL STUDY ABOUT THE CHANGE OF SUBMANDIBULAR GLANDULAR CELL IN WHITE RAT FOLLOWING $HgCl_{2}$ INJECTION

승홍 투여가 백서 악하선 세포의 조직병리학적 변화에 미치는 영향에 관한 연구

  • Kim, In-Su (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University) ;
  • Kim, Kyung-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Dankook University)
  • 김인수 (단국대학교 치과대학 구강악안면외과학 교실) ;
  • 김경욱 (단국대학교 치과대학 구강악안면외과학 교실)
  • Published : 2002.12.31
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Abstract

Mercury is one of the most frequently used heavy metal in dental clinic. Mercury poisoning rises up when someone is exposed to mercury chronically. In 1818, Amalgam was used for dental restorative procedure, and after then study about mercury toxicity has begun. Clinical signs of mercury toxicity in oral & maxillofacial area were increases of salivation, metallic taste, swelling and pain of tongue, redness and ulceration of oral mucosa, and increased mobility and loss of teeth. After we injected mercury($HgCl_{2}$) into intraperitoneum of rat, studied about histopathological changes of submandibular gland cell. Experimental group was divided into two groups by amount of mercury. (Group 1 was 0.5mg/Kg of mercury injection, group 2 was 1.0mg/Kg of mercury injection.) 1. After 3days of intraperitoneal injection, black granules were observed at macrophage cell in both group. In group 2, author found hyperchromatism of nucleus, and vacuolization of cellular matrix and nucleus of acinar cell. 2. After 1week of intraperitoneal injection, author found severe vacuolization of nucleus and cellular matrix, and irregular granules around nuclear membrane at mucous cell and serous cell in both group. Vacuolization of nucleus and cellular matrix was seen at duct cell in group 2. 3. After 2weeks of intraperitoneal injection, author could found severe vacuolization of cellular matrix, and sometimes nucleus was positioned in central area of cellular matrix at mucous and serous cell in both group. Vacuolization of nucleus and cellular matrix was found at vascular endothelial cell in group 2. 4. After 4weeks of intraperitoneal injection, destruction and distortion of gland cells were distinct. Vacuolization and destruction of nucleus and cellular matrix was found at duct cell in group 2. After intraperitoneal injection of mercury, we found equanimity of mercury and destruction of cellular matrix at serous cell, mucous cell, and duct cell of submandibular gland. So, we thought that metallic taste of mercury poisoning patient would be due to excretion of saliva containing mercury.

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References

  1. Khordi MM, Sarraf SA, Balali MM : Urinary mercury excretion following amalgam filling in children. J Toxicol Clin Toxicol. 39(7) : 707-710, 2001 https://doi.org/10.1081/CLT-100108511
  2. Dental Material, Junga publish, chaper 10, dental amalgam p254-283
  3. Hol PJ, Vamnes JS : Dental amalgam and selenium in blood. Environ Res. 87 : 141-146, 2001 https://doi.org/10.1006/enrs.2001.4308
  4. Takahashi Y, Tsuruta S, Hasegawa J : Release of mercury from dental amalgam fillings in pregnant rats and distribution of mercury in maternal and fetal tissues. Toxicology. 21 : 115-126, 2001
  5. Razagui IB, Haswell SJ : Mercury and seleniumconcentrations in maternal and neonatal scalp hair: relationship to amalgam-based dental treatment received during pregnancy. Biol Trace Elem Res. 81 : 1-19, 2001 https://doi.org/10.1385/BTER:81:1:01
  6. Jacobs MB, Ladd AC, and Goldwater LJ : Absorption of mercury in man. Dental amalgams as a source of urinary mercury. Arch Environ Health. 9 : 454-463, 1984
  7. Cook TA, Yates PD : Fatal mercury intoxification in a dental surgery assistant. Br. Dent. J 127 : 53, 1969
  8. Bjarne MM : Localization of mercury in CNS of the rat. Toxicol Appl Pharmacol. 103 : 303-323, 1990 https://doi.org/10.1016/0041-008X(90)90232-J
  9. M Mehra, B H Choi : Distribution of mercury in subcellular fractions of brain, liver, and kidney, after repeated oral administration of 203Hg-labeled methylmercuric chroride in mice. Experimental and molecular pathology. 35 : 435-447, 1981
  10. T Norseth : The intracellular distribution of mercury in rat liver after a single injection of mercuric chloride. Biochemical pharmacology. 17 : 581-593, 1968
  11. J Jonek, A Pacholek, and W Jez , Arch Gewerbepath Gewerbehyg. 20 : 562, 1964
  12. Warfvinge G, Warfvinge K, Larsson A : histochemical visualization of mercury in the oral mucosa, salivary and lacrimal glands of BN rats with HgCl2 induced autoimmunity. Exp. Toxicol. Pathol. 46 : 329-334, 1994
  13. Bente L, Rasmussen, Ole T : Ultrastructural localization of mercury in adrenals from rats exposed to methyl mercury. Virchows Arch. 52 : 529-538, 1987
  14. P Bratt et al : Function of the rat salivary glands after exposure to inorganic mercury. the science of total envir. 172 : 47-55, 1995