Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effect of Hwangryunhaedok-tang on Blood Pressure and Renal Functions in Spontaneously Hypertensive Rats

황연해독탕(黃連解毒湯)이 자발적(自發的) 고혈압(高血壓) 백서(白鼠)의 혈압(血壓) 신장(腎臟) 기능(機能)에 미치는 영향(影響)

  • Kook Yoon-Bum (Department of Oriental Medical Prescription Sangji University)
  • 국윤범 (상지대학교 한의과대학 본초방제학교실)
  • Published : 2002.06.20
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Abstract

The present study designed to investigate whether hwangryunhaedok-tang show an anti-hypertensive effect and elucidate its possible mechanism in spontaneously hypertensive rats. The systolic blood pressures (SBP) were significantly decreased as an oral administration of hwangryunhaedok-tang compared with their control group. The urine volume was significantly increased by administration of hwangryunhaedok-tang but urinary sodium (UNaV), potassium (UKV), chloride excretion (UCIV) were not remarkably affected. The urinary creatinine excretion rate (UcrV) was significantly increased in rats administered with hwangryunhaedok-tang in association with increase of creatinine clearance (Ccr). The urine osmolality (Uosmol) was significantly decreased in SHR administered with hwangryunhaedok-tang without being changed in solute-free water reabsorption ( TcH20). The expressions of Aquaporin 2 (AQP-2). 3 and ${\alpha}\;1$, ${\beta}\;1$ subunits of Na.K-ATPase were determined by Western blot analysis to assess the role of these proteins in association with changes of renal functions in SHR administered with hwangryunhaedok-tang. The expression of AQP-2 and 3 protein was significantly down-regulated in the kidney of SHR administered with hwangryunhaedok-tang compared with those in control rats without being altered expression of ${\alpha}\;1$, ${\beta}\;1$ subunits of Na,K-ATPase. In the in vitro assay, Angiotensin converting enzyme (ACE) was inhibited by hwangryunhaedok-tang in a dose-dependent manner. Berberine and/or palmatine, which are well known as a main components of hwangryunhaedok-tang, also have an ACE inhibitory effects in a dose-dependent manner. Taken together, these results suggest that hwangryunhaedok-tang lowered blood pressure through the increase of diuresis caused by down-regulation of water channels and the inhibition of Angiotensin converting enzyme.

Keywords

References

  1. 주後備急方 葛仙翁
  2. 最新韓方臨床學 裵元植
  3. 黃連解毒湯 엑기스가 家兎의 體溫 및 血壓에 미치는 影響 鄭遇悅
  4. 黃連解毒湯煎液이 thioacetamides 毒性으로 인한 代謝 障碍에 미치는 影響에 대한 實驗的 硏究 崔大善
  5. 黃連解毒湯이 緬羊赤血球에 대한 免疫反應에 미치는 影響 宋昊埈
  6. 黃連解毒湯 藥針製劑의 安定性 및 效能에 關한 硏究 辛吉祚
  7. 黃連解毒湯加味方에 대한 免疫 글로블린 E 媒介性 아나필락시의 억제 尹星燦
  8. 賢과 賢病의 證治 李兆華
  9. 韓國食品科學會誌 v.29 no.6 k-Casein의 chymosin, pepsin 및 trypsin 및 加水 分解物에 대한 안지오텐신 變煥 酵素 沮害 效果의 探索 오세종;김세현;김상교;백영진;조경현
  10. 韓國食品科學會誌 v.30 no.6 Chitosan 올리고당의 안지오텐신 轉換酵素 活性抑制 및 SHR에서의 高血壓 抑制 特性 홍상필;김명희;오세욱;한찬규;김용현
  11. 臨床本草學 辛民敎
  12. 外臺秘要 王燾
  13. 劉河間傷漢三六書 劉完素
  14. 奇效良方 方賢
  15. 醫方集解 汪認庵
  16. 東醫心系內科學(上) 李京燮(등)
  17. 現代 生藥學 生藥學硏究會
  18. Harrison's 內科學(Ⅱ) Harrison
  19. 慶熙韓醫大 論文集 v.5 杜鎬京;朴憲在
  20. 대한한의학회지 v.20 no.1 黃蓮解毒湯과 溫淸飮이 高血壓 및 高脂血證에 미치는 影響 金恩善;朴致相;朴昌國
  21. 生理學 김종규
  22. 辨證論治 方藥合編 黃道淵
  23. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.M.
  24. Hypertension v.7 no.2 An improved flurometric assay of rat serum and plasma converting enzyme Santos, R.A.;Krieger, E.M.;Greene, L.J.
  25. 전정판 腎臟學 서울대학교 의과대학
  26. Pflugers Arch. v.329 The localization of the $\Na^{+}-K^{+}$-ATPase in the cells of rat kidney cortex Kinne, R.;Schmitz, J.E.;Kinne, S.E.
  27. Curr. Probl. Clin. Biochem. v.3 $\Na^{+}$, $\K^{+}$-ATPase in the rat nephron related to sodium transport: result with quantitative histochemistry Schmidt, U.;Dubach, U.C.
  28. 人體生理學 김광진;김창주;김형진;박사훈
  29. J. Biol. Chem. v.269 Na, K-ATPase Lingrel, J.B.;Kuntzweiler, T.
  30. J. Biol. Chem. v.263 Tisue-specific and developmental regulation of rat Na, K-ATPase catalytic alpha isoform and beta subunit mRNAs Orlowski, J.;Lingrel, J.B.
  31. Am. J. Physiol. v.264 High-salt diet upregulates activity and mRNA of renal $\Na^{+}-K^{+}$-ATPase in Dahl salt-sensitive rats Nishi, A.;Celsi, G.;Aperia, A.
  32. Hypertension v.28 Renal Na, K-ATPase in genetic hypertension Ferrandi, M.;Tripodi, G.;Salardi, S.;Florio, M.;Modica, R.;Barassi, P.;Parenti, P.;Shainskaya, A.;Karlish, S.;Bianchi, G.;Ferrari, P.
  33. Am. J. Hypertension v.10 Renal sodium pump regulation in deoxycorticosterone salt hypertension in the rat Soszynski, P.A.;Tao, Q.F.;Krep, H.H.;Hollenberg, N.K.;Graves, S.W.
  34. Pharmacological Research v.41 no.1 Upregulation of Renal $\Na^{+}-K^{+}$-ATPase in the L-NAME-induced hypertensive rats Kang, D.G.;Kim, J.W.;Lee, J.U.
  35. Kidney Int. v.57 no.4 Diminished adenylate cyclase activity and aquaporin 2 expression in acute renal failure rats Kim, S.W.;Jeon, Y.S.;Lee, J.U.;Kang, D.G.;Kook, H.;Ahn, K.Y.;Kim, S.Z.;Cho, K.W.;Kim, N.H.;Han, J.S.;Choi, K.C.
  36. J. Clin. Invest. v.99 no.7 Upregulation of auqaporin-2 water channel expression in chronic heart failure rat Xu, D.L.;P.Y Martin;M. Ohara;J. St John;T. Pattison;X. Meng;K. Morris;J.K. Kim;R.W. Schrier
  37. Kidney Int. v.53 Impaired auaporin and urea transporter expression in rats with adriamicine-induced nephrotic syndrome Fernadez-llama, P.;Andrews, P.;Nielsen, S.;Ecelbarger, C.A.;Knepper, M.A.
  38. J. Clin. Invest v.102 no.1 An aquaporin-2 water channel mutant which causes autosomal dominant nephrogenic diabetes insipidus is retained in the Golgi complex Mulders, S.M.;Bichet, D.G.;Rijss, J.P.;Kamsteeg, E.J.;Arthus, M.F.;Longergan, M.;Fujiwara, M.;Morgan, K.;Leijendekker, R.;van der Sluijs, P.;van Os C.H.;Deen, P.M.
  39. Am. J. Physiol. v.277 no.3 Reduced adundance of aquaporins in rats with bilateral ischemiainduced acute renal failure: prevention by alpha-MSH Kwon, T.H.;Frokiaer, J.;Fernandez-Llama, P.;Knepper, M.A.;Nielsen, S.
  40. Clin. Exp. Hypertens. v.22 no.5 Increased expression and shuttling of aquaporin-2 water channels in the kidney in DOCA-salt hypertensive rats Lee, J.U.;Kang, D.G.;Kim, Y.J.
  41. Annu. Rev. Biochem. v.45 Angiotensin-converting enzyme and the regulation of vasoactive peptides Soffer, R.L.
  42. Biochem. Pharmacol. v.20 no.7 Bradykinin potentiating peptide PCA-Lys-Trp-Ala-Pro. An inhibitor of the pulmonary inactivation of bradykinin and conversion of angiotensinⅠto II Stewart, J.M.;Ferreira, S.H.;Greene, L.J.
  43. Kor. J. Pharmacog. v.12 no.1 Screning of Plant Materials for the inhibitory activities against angiotensin cinverting enzyme Yun, H.S.;Chung, S.H.;Han, B.H.
  44. Chem. Pharm. Bull. v.32 no.9 Inhibitors of angiotensin converting enzyme in crude drugsⅠ Inokuchi, J.;Okabe, H.;Yamauchi, T.;Nagamatsu, A.
  45. Life Sciences v.38 Antihypertensive substance in seeds of Areca catechu L Inokuchi, J.;Okabe, H.;Yamauchi, T.;Nagamatsu, A.;Nonaka, G.;Nishioka, I.
  46. J. Ethnopharmacol. v.60 Ethmomedical information and in vitro screening for angiotensin-convertiong enzyme inhibition of plants utilized as traditional medicines in Gujarat, Rajasthan, and Kerala Nyman, U.;Joshi, P.;Madsen, L.B.;Pederses, T.B.;Pinstrup, M.;Rajasekharan, S.;George, V.;Pushpangadan, P.
  47. J. Etrhnopharmacol. v.58 Plants from reunion island with allefed antihypertensive and diuretic effects-an experimental and ethnobotanical evaluation Adsersen, A.;Adsersen, H.
  48. J. Ethnopharmacol. v.68 Screening of Zulu medicinal plants for angiotensin converting enzyme(ACE) inhibitiors Duncan, A.C.;Jager, A.K.;van Stan, J.
  49. J. Ethnopharmacol. v.48 In vitro screening of traditional medicines for anti-hypertensive effects based on inhibition of the angiotensin converting enzyme Hansen, K.;Nymann, U.;Smitt, U.W.;Adersen, A.;Gudiksen, L.;Rajasekharan, S.;Pushpangadan, P.
  50. J. Natl. Prod. v.51 Chemical and pharmceutical suudies on medicinal plants in Paraguay;Geraniin: an angiotensin-converting enzyme inhibitor from 'Paraparai mi', Phyllanthanthus niruri Uneo, H.;Horie, S.;Nishi, Y.;Shogawa, H.;Kawasaki, M.;Suzuki, S.;Hayashi, T.;Arisawa, M.;Shimizu, M.;Yoshizaki, M.;Mirita, N.
  51. Planta. Med. v.58 ACE-inhibitory procyanidins from Lesoedeza capitata Wagner, H.;Elbl, G.
  52. Pharmaceutical Pharmacol. Lett v.1 Evaluation of natural products as inhibitors of angiotensin I-convertion enzyme(ACE) Wagner, H.;Elbl, G.;Lotter, H.;Guinea, M.
  53. 한국식품과학회지 v.25 no.3 한국산 녹차로 부터 분리한 Flavan-3-ol 化合物의 angiotensin converting enzyme 沮害 效果 조영재;안봉천;최청
  54. J. Natl. Prod. v.50 no.4 Inhibitory effects of various flavonoids isolated from leaves of persimon on angiotensin-converting enzyme activity Kamaeda, K.;Takaku, T.;Okuda, H.;Kimura, Y.;Okuda, T.;Hatano, T.
  55. J. Natl. Prod. v.55 no.5 Inhibition of angiotensin-Ⅰ-converting enzyme by tetrahydroxyxanthones isolated from Tripterospermum lanceolatum Chen, C.H.;Lin, J.Y.
  56. Shoyakugaku Zassi v.41 Studies on the crude drug containing the angiotensin-converting enzyme inhibitors I. On the active principles of Lycium chinense Muller Morota, T.;Sasaki, H.;Chin, M.;Sato, T.;Katayama, N.;Fukuyama, K.;Mitsuhashi, H.
  57. Chem. Pharm. Bull. v.34 no.7 Angiotensin cinverting enzyme-inhibitory triterpenes from Gaboderma lucidum Morigiwa, A.;Kitabatake, K.;Fujimoto, Y.;Ikekawa, N.
  58. 大韓韓醫學會誌 v.22 no.2 韓藥材들의 안지오텐신 轉換酸素 抑制劑 作用의 檢索 강대길;오현철;손은진;권태오;이호섭