The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

An Anti-Tumor Effects of Dioscorea bulbifera L. on Stomach Cancer Cells

황약자(黃藥子)의 위암세포(胃癌細胞)에 대(對)한 항암(抗癌) 효능(效能) 검색(檢索)

  • Ryu, Bong-Ha (Dept. of 3rd Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Ryu, Ki-Won (Dept. of 3rd Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Yoon, Sang-Hyub (Dept. of 3rd Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Kim, Jin-Seong (Dept. of 3rd Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hee (Dept. of 3rd Internal Medicine, College of Oriental Medicine, Kyunghee University)
  • 류봉하 (경희의료원 부속한방병원 3내과) ;
  • 류기원 (경희의료원 부속한방병원 3내과) ;
  • 윤상협 (경희의료원 부속한방병원 3내과) ;
  • 김진성 (경희의료원 부속한방병원 3내과) ;
  • 김성희 (경희의료원 부속한방병원 3내과)
  • Published : 2002.03.30
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Abstract

Background : Nowadays a lot of research is based on natural substances or materials world wide since many kinds of side effects are accompanied by anti tumor chemotherapy. In Chinese medicine, Dioscorea bulbifera L is widely used to treat many kinds of cancer, but in Korea it is rarely used. Therefore, we need to scientifically identify anti tumor effects of Dioscorea bulbifera L. Objective : We aimed to identify anti tumor effects of Dioscorea bulbifera L on the stomach cancer cells through molecular biological methods. Materials & Methods : We used AGS, a stomach cancer cell from American Type Culture Collection. We injected the boiled extract of Dioscorea bulbifera L 5 ul(sample 1), 10ul(sample 2) to cultured media(ml) for 0,6,12,18,24 hours. We measured the killing effect on stomach cancer cells through Tryphan blue exclusion test and suppressive effect on viability of stomach cancer cells via MTT assay. Results : Tryphan blue exclusion test showed that each test group killed more stomach cancer cells than the controlled group with a dosage-dependent, but not significantly. MTT assay showed that each test group had a more suppressive effect on viability of stomach cancer cells than the controlled group without a dosage-independent, but not significantly. The cell cycle analysis via flow cytometry showed that the test group extended cell cycle, and there was no peak in M phase, the number of sub G1, G0, G1 phase cells increased a little, but not significantly. Conclusion : This experiment showed that Dioscorea bulbifera L. has an anti-tumor effect, but not significantly. This is in vitro experiment and basic experiment on Dioscorea bulbifera L. We hope more progressive researches on Dioscorea bulbifera L. will be conducted and its anti tumor will be more accurately identified.

Keywords

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