The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Identification of Entomopathogenic Bacteria for Biological Control of the Mushroom Fly, Lycoriella mali

느타리 재배에서 버섯파리의 생물학적 방제를 위한 곤충병원성 세균의 분리 및 동정

  • Lee, Su-Hee (Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Lim, Eun-Kyung (Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Choi, Kwang-Ho (Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Jae-Pil (Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Hyun-Ouk (Faculty of Natural Resources and Life Science, Dong-A University) ;
  • Kim, Ik-Soo (Sericulture & Entomology, National institute of Agricultural Science and Technology) ;
  • Moon, Byung-Ju (Faculty of Natural Resources and Life Science, Dong-A University)
  • 이수희 (동아대학교 생명자원과학부) ;
  • 임은경 (동아대학교 생명자원과학부) ;
  • 최광호 (동아대학교 생명자원과학부) ;
  • 이재필 (동아대학교 생명자원과학부) ;
  • 이현욱 (동아대학교 생명자원과학부) ;
  • 김익수 (농업과학기술원 잠사곤충부) ;
  • 문병주 (동아대학교 생명자원과학부)
  • Published : 2002.04.30
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Abstract

The study was conducted to isolate and identify insecticidal bacteria for biological control of larvae of mushroom fly, Lycoriella mali, which is one of serious pests to oyster mushrooms during its cultivation period. Among eight bacteria isolated from the soil in the oyster mushroom beds and the dead body of L. mali, two bacteria, Bti-D and Bti-U showed more toxicity with mortality rate than other six-bacteria isolates. The two bacteria showed more toxicity in three instar of the period of development of the mushroom fly than in other instar. Symptoms of the larvae of L. mali infected by the two bacteria developed as follows: at the early infection, the front middle gut changed color to light brown, the middle gut to brown, whole body to black brown, and eventually, the fly died. For the identification of these isolates, cultural and biochemical characteristics by Bergey's manual and Biolog system, cell morphology by TEM, endospore and endotoxin by phase-contrast microscope, and test using 33H antisera were examined. According to the results, these two isolates, Bti-D and Bti-U were identified as Bacillus thuringiensis subsp. israelensis respectively.

느타리버섯 재배에 있어서 주요해충인 버섯파리 Lycoriella mali의 생물학적 방제를 위하여 전국 느타리버섯 재배지와 버섯파리의 병사충에서 버섯파리 유충에 병원성을 갖는 세균 8균주를 분리하였다. 이 중 Bti-D 및 Bti-U 2균주에 의한 사충율이 각각 82.3%와 87.3%로 가장 높았다. 버섯파리 유충의 발육단계에 따른 살충 효과 검정에서는 두 균주 모두 3령 층에서 높은 살충효과를 보였다. 이들 세균으로 감염된 유충에 나타나는 병징으로서 감염초기에는 중장의 앞부분이 연한 갈색을 띄고, 차츰 중장의 뒷부분까지 진행되어 전 부분이 흑갈색으로 변색되면서 치사되었다. 이들 두 균주를 동정하기 위하여 배양적, 생화학적 및 생리적 특성을 조사하고 Bergey's manual과 Biolog system을 동정에 이용하였으며, 주사전자현미경으로 세포형태를, 위상차현미경으로 내생포자와 내독소를 관찰하여 판정한 결과, 두 균주 모두 Bacillus thuringiensis로 동정되었으며, 편모항원성을 조사한 결과, B. l. subsp. israelensis로 동정되었다.

Keywords

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