The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Analysis of Genetic Relationship and Cultural Characteristics of Penicillium species Isolated from Postharvest Decay of Pear by Random Amplified Polymorphic DNA

저장 배에서 분리한 Penicillium속의 배양적 특성과 RAPD에 의한 유연관계 분석

  • Kim, Ju-Hee (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Lee, Wang-Hyu (Faculty Biological Resources Science, Chonbuk National University) ;
  • Ryu, Young-Jin (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Cheong, Seong-Soo (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi, Joung-Sik (Jeollabuk-do Agricultural Research and Extension Services)
  • 김주희 (전라북도 농업기술원) ;
  • 이왕휴 (전북대학교 농과대학 생물자원과학부 농업과학기술부) ;
  • 유영진 (전라북도 농업기술원) ;
  • 정성수 (전라북도 농업기술원) ;
  • 최정식 (전라북도 농업기술원)
  • Published : 2002.10.30
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Abstract

Fifteen isolates from pear, and sixteen isolates from KCTC, KCCM, and Chungnam Univ. of Penicillium spp. were investigated for the analysis of their relationships of cultural characteristics and RAPD genetic variation by RAPD. The cultural characteristics of Penicillium spp. were shown different growth rate, morphology, and color. In addition, the cultural characteristics and RAPD analysis were conducted for the pear rot pathogens and related isolates. RAPD patterns were applied to compare the taxonomic and genetic diversity of the Penicillium species between 15 groups isolated from pear fruits and 16 standard species. The genomic DNA were amplified from $0.1{\sim}2.0kb$ by five URP primer and 744 bands were detected. The cluster analysis showed four genomic DNA RAPD groups and its similarity was 47.7%. Intraspecific relationships were 87.4, 97.5 and 95.2%, in P. expansum, P. solitum, and P. crustosum, respectively. These results appeared to be that there were high similarities between isolates, and consistent with the results of cultural morphological characteristics analysis.

저장배에서 분리한 15그룹 3종을 KCTC, KCCM, 충남대에서 분양받은 16균주를 대상으로 상호 유연관계를 분석하고자 배양적 특성을 관찰하고 RAPD 검정에 의한 진단 가능성을 검토하였다. 병원균의 배양특성을 조사한 결과 배지상 생육형태, 색, 속도 등에 있어서 균주간 차이를 보였으며 배양적 특성과 RAPD에 의한 유연관계 분석 결과를 비교 분석하기 위하여 배에서 분리한 15개 균주와 16개 표준 균주의 종간 다형성을 관찰하였다. RAPD을 이용하여 유전적 다양성을 분석하기 위하여 5개의 URP primer를 이용하여 genomic DNA를 증폭시킨 결과 $0.1{\sim}2.0kb$ 크기의 총 744개 band들이 증폭되었다. cluster 분석결과 전체 유사도는 47.7%로 31 균주들은 4개의 genomic DNA RAPD 집단으로 분류되었다. 배에서 분리한 P. expansum 균주간에는 87.4% 이상의 상동성을 보였고, P. solitum 균주간에는 97.5%, P. crustosum 균주 간에는 95.1% 이상의 상동성을 보였다. 배에서 분리된 동일한 종내에서는 배양적 특성에 의한 동정결과와 RAPD에 의한 분석결과와 일치하였다.

Keywords

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