The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Production of Lentinus edodes Mycelia in Submerged Culture and It's Hypoglycemic Effect in Diabetic Rats

표고버섯 균사체의 생산과 당뇨 유발 흰쥐에 대한 혈당강하 효과

  • Yang, Byung-Keun (Research Center for Processing & Application of Agricultural Products, Taegu University) ;
  • Kim, Dong-Hyun (Department of Biotechnology, Taegu University) ;
  • Song, Chi-Hyun (Department of Biotechnology, Taegu University)
  • 양병근 (대구대학교 농산물 저장.가공 산업화 연구센터) ;
  • 김동현 (대구대학교 생물공학과) ;
  • 송치현 (대구대학교 생물공학과)
  • Published : 2002.10.30
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Abstract

The optimum conditions for the production of Lentinus edodes mycelia and it's hypoglycemic effect was studied. Optimum pH and temperature for the production of mycelia in shaken flask culture were 5 and $30^{\circ}C$, respectively, for 24 days cultivation. Culture period for maximum production of mycelia (8.13 g/l) in 5-l jar fermenter cultivation was shortened as much as 6 days compared to shaken flask culture. The mycelial dose of 5 % proved almost equally effective in lowering the plasma glucose, total cholesterol, and triglyceride level as much as 23.0, 20.7, and 27.1%, respectively. The values of alanine transaminase and aspartate transaminase were also estimated and exhibited a substantial reduction (29.6 and 34.3%, respectively) in activity after the administration of dried mycelia.

표고버섯 균사체가 항당뇨 효과에 미치는 영향을 검토하기 위해 당뇨가 유발된 흰쥐에 표고버섯 균사체을 7일간 급여시킨 후 혈장 glucose, total cholesterol, triglyceride 및 ALT, AST 활성을 관찰하였고, 표고버섯 균사체 생장의 최적 배양 조건을 조사하였다. 균사체 생장을 위한 최적의 pH와 온도는 5와 $30^{\circ}C$로 나타났으며, 발효조배양시에는 최대 균사체 생장(8.13g/l)이 진탕배양일 때보다 6일 단축되었다. Hypoglycemic 효과에서 혈장 glucose는 당뇨 유발군에서 표고버섯 균사체 급여군이 대조군보다 23.0%가 유의하게 감소하였으며, 혈장 total cholesterol과 triglyceride는 20.3%와 27.1%로 낮게 나타났다. 또한 혈장 ALT와 AST 활성은 당뇨 대조군에 비해 표고버섯 균사체 급여군이 29.6%와 34.3% 낮은 활성을 보였다.

Keywords

References

  1. Allain, C. C., Poon, L. S. and Richmond, W. 1974. Enzymatic detennination of total serum cholesterol. Clin. Chem. 20: 470-475.
  2. Bailey, C. J. and Day, C. 1989. Traditional plant medicines as treatments for diabetes. Diabetes Care 12: 553-559. https://doi.org/10.2337/diacare.12.8.553
  3. Baron, H. Levy, E., Oschry, Y. Ziv, E. and Scafrir, E. 1984. Removal effect of very low density lipoproteins from diabetic rats. Biochem. Biophysi. Acta. 793: 115-118. https://doi.org/10.1016/0005-2760(84)90059-6
  4. Beppu, H., Maruta, K., Kurner, T, and Kolb, H. 1987. Diabetogenic action of streptozotocin: essential role of membrane permeability. Acta. Endocrinol (Copdhn) 114: 90-96.
  5. Bucolo, G. and David, H. 1973. Quantative determination of serum trig1ycerides by use of enzyme. Clin. Chem. 19: 476-482.
  6. Chihara, G., Maeda, Y, Hamuro, J., Sasaki, T. and Fukuoka, F. 1969. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) Sing. Nature 17: 687-688. https://doi.org/10.1038/222687a0
  7. Cho, Y. J., Kim, H. A., Bang, M. A. and Kim, E. H. 2002. Effects of dietary mushroom on blood glucose levels, lipid concentrations and glutathione enzymes in streptozotocininduced diabetic rats. Kor. J. Nutr. 35(2): 183-191.
  8. Chung, K. S. 1982. Studies on the constituents and culture of the higher fungi of Korea (II); The antitumor components and culture of Lentinus edodes (Berk.) Singer. Kor. J. Mycol. 10(1): 33-39.
  9. Duncan, D. M. 1957. Multiple-range tests for correlated and heteroscedastic means. Biometrics 13: 164-170. https://doi.org/10.2307/2527799
  10. Junod, A. Lambert, A. E., Orci, L., Pictet, R., Gonet, A. E. and Renold, A. E. 1967. Studies of the diabetogenic action of streptozotocin. Proc. Soc. Exp. Biol. Med. 126: 201-205. https://doi.org/10.3181/00379727-126-32401
  11. Lee, B. W., Im, G. H., Kim, D. W., Park, K. M., Son, S. H., and Shon, T. H. 1993. Culture characteristic and pilot scale fermentation for the submerged mycelial culture of Lentinus edodes. Kor. J. Microbiol Biotechnol. 21(6): 609-614.
  12. Lee, K. S., Sea, J. S., Choi, Y. S. 1998. Effect of Sea tangle and hypoglycemic agent on lipid metabolism in diabetic rats. J. Korean Soc. Food Sci. Nutr. 27: 960-967.
  13. Maeda, Y. and Chihara, G. 1971. Lentinan a new immuno-accelerator of cell mediated responses. Nature 229: 634-639. https://doi.org/10.1038/229634a0
  14. Park, K. S. and Lee J. S. 1991. Optimization of media composition and culture conditions for the mycelial growth of Coriolus versicolor and Lentinus edodes. Korean J. Biotechnol. Bioeng. 6(1): 91-98.
  15. Raabo, E. and Terkildsen, T. C. 1960. On the enzymatic determination of blood glucose. Scand. J. Clin. Lab. Invest. 12: 402-407. https://doi.org/10.3109/00365516009065404
  16. Reitman, S. and Frankel, S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathol. 28: 56-63. https://doi.org/10.1093/ajcp/28.1.56
  17. Rho, H. M., Choi, M. A. and Koh, J. B. 1998. Effects of raw soy flour (yellow and black) on serum protein concentrations and enzyme activity in Streptozotocin-diabetic rats. J. Korean Soc. Food Sci. Nutr. 27: 724-730.
  18. Saudek, C. D. and Eder, H. A. 1979. Lipid metabolism in diabetes mellitus. Am. J. Med 66: 843-849. https://doi.org/10.1016/0002-9343(79)91126-4
  19. Song, C. H., Yaog, B. K., Jeoo, Y. J., Ra, K. S., Shon, D. H., Kim, H. I. and Kim, Y. H. 1998. The optimum conditions for the production of exo-polymer from submerged mycelial culture of Ganoderma lucidum WK-003 and It's hepatoprotective effect. Korean J. Biotechnol. Bioeng. 13(4): 364-368.
  20. Song, C. H. and Cho. K. Y. 1987. A synthetic medium for the production of submerged culture of Lentinus edodes. Mycologia 76: 866-876. https://doi.org/10.2307/3807688
  21. Suzuki, H., Iiyama, K., Yoshida, O., Yamazaki, S., Yamamoto, N., and Toda, S. 1990. Structural characterization of the immuno active and antiviral water-solubilized lignin in an extract of the culture medium of Lentinus edodes mycelia (LEM). Agric. Biol. Chem. 54: 479-487. https://doi.org/10.1271/bbb1961.54.479
  22. Suzuki, S. and Oshima. S. 1976. Influence of Shitake (Lentinus edodes) on human semrn cholcsterol. Mushroom Science 9: 463-467.
  23. Tamura, R., Tanebe, K., Kawanishi, C., Torii, K. and Ono, T. 1997. Effects of lcntinan on abnormal ingestive behaviors induced by rumer necrosis factor. Physiology & Behavior 61: 399-404. https://doi.org/10.1016/S0031-9384(96)00451-9
  24. Tol, A. V. 1977. Hypertriglyceride in the diabetic fat deffective removal of serum very low density lipoprotein. Atherosderosis 26: 117-128. https://doi.org/10.1016/0021-9150(77)90146-0
  25. Wahren, J., Felig, P., Cerasi, E. and Luft, R. 1972. Splanchnic and peripheral glucose amino acid metabolism in diabetes mellitus. J. Clin. Invest., 51: 870-876. https://doi.org/10.1172/JCI106989
  26. Woo, J. Y., Baek, K. Y., Han, J. P. 1998. Effect of Royal jelly on therapy and prevention of streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 27: 1267-1272.
  27. Yamamura, Y. and Cochrane, K. W. 1976. A selective inhibitor of myxoviruses from Shiitake (Lentinus edodes).Mushroom Science 9: 495-507.
  28. Yang, B. K., Kim, D. H., Jeong, S. C., Das, S., Choi, Y. S., Shin, J. S., Lee, S. C., ande Song, C. H. 2002. Hypoglycemic effect of a Lentinus edodes exo-polymer produced from a submerged mycelial culture. Biosci. Biotechnol. Biochem. 66(5): 707-712. https://doi.org/10.1271/bbb.66.937

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