Annals of Surgical Treatment and Research

The Korean Surgical Society (대한외과학회)
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Mast Cells in Renal Allografts

이식신 생검에서 비만 세포(Mast Cells)의 임상적 의의

Lee, Jae-Choon;Lee, Sam-Uel;Park, Sung-Gil;Kim, Seung-Il;Lee, Young-Cheol;Kim, Joo-Seop;Joo, Sun-Hyung;Oh, Kook-Hwan;Chae, Dong-Wan;Nam, Eun-Sook;Park, Chul-Jae
이재춘;이삼열;박성길;김승일;이영철;김주섭;주선형;오국환;채동완;남은숙;박철재

  • Published : 20020900
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Abstract

Purpose: Chronic rejection is the enemy in the battle for long term survival after renal allografts. Interstitial fibrosis is known to be the important finding in renal allografts with chronic rejection. Mast cells secrete a large number of fibrogenic factors and have been involved in chronic inflammation and tissue fibrosis. In this study the authors evaluated the relationship between mast cells and fibrosis in renal allografts with chronic rejection. Methods: The authors evaluated 42 biopsied specimens of renal allografts. Immunohistochemistry using anti-mast cell tryptase (Dako, 1:200) and an LSAB kit (Dako) was applied to detect mast cells. The mean number of mast cells (MNM) per 10 high power fields was counted. Results: MNM of implantation biopsies was 0.640$\pm$0.537, of acute rejection -1.969$\pm$1.216, of chronic rejection -6.0$\pm$3.133 (P<0.01), of acute tubular necrosis -1.360$\pm$0.899, and of acute cyclosporine nephrotoxicity -1.000$\pm$0.600. MNM according to donor source was 3.267$\pm$3.479 vs. 2.376$\pm$1.900 (living donors vs. cadaveric donors). MNM was significantly correlated with donor sex (male?: female ratio of = 2.319$\pm$1.739 : 4.014$\pm$4.286, P<0.01), and cholesterol (hypercholesterolemia vs. non-hypercholesterolemia, 4.125$\pm$5.497 vs. 2.60$\pm$1.916, P<0.01). However, MNM according to blood pressure was not statistically significant (hypertension : non-hypertension ratio of=3.189$\pm$3.05 : 1.200$\pm$1.226, P>0.05). Conclusion: Our data show that the number of mast cells in renal allograft was significantly associated with chronic rejection, donor sex and hypercholesterolemia.

Keywords

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