The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Several Herbs on Acetylcholinesterase Enzyme Activity

數 種 韓藥의 아세틸콜린에스터레이즈 酵素活性에 대한 효과

Choi Jae-Hwan;Kim Mi-Young;Kim Hyun-Mi;Cui Xun;Jeon Hoon;Kim Dae-Keun;Lim Jong-Pil;Leem, Kang-Hyun
최재환;김미영;김현미;최훈;전훈;김대근;임종필;임강현

  • Published : 20020000

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Abstract

아세틸콜린에스터레이즈(AChE)는 신경조직에 분포하는 효소로 신경전달물질 중 아세틸콜린(ACh)을 분해하는 효소로 시냅스 신호전달체계에 있어 중요한 역할을 하는 효소이다. 현재 알츠하이머병과 치매치료에 사용하는 서양약물은 AChE의 활성을 저해하는 작용기전을 가진다. 목적 : 전통적으로 기억력 감퇴, 치매 등 치료에 사용되어 온 한약의 AChE에 대한 작용을 밝히기 위해 본 실험이 구상되었다. 방법 : 생쥐 대뇌신경세포의 균질 혼합액으로부터 효소의 활성을 Ellman 방법을 개선한 엘리자(ELISA)법을 이용하여 측정하였다. 결과 : 실험결과 天花粉 의 6종이 2.5mg/ml의 농도에서 우수한 효소활성저해력을 보였고, 50% 효소활성 저해농도(ID_50)를 구한 결과, 天花粉의 $ID_50$은 1.5mg/ml이었고, 赤芍藥 1.2 mg/ml, 玄蔘 2.0 mg/ml, 魚腥草 1.2 mg/ml, 忍冬藤 1.0 mg/ml, 桑寄生 1.7mg/ml, 및 大服皮 2.0 mg/ml를 나타내어 忍冬藤의 저해력이 가장 우수하였다. 결론 : 치매 및 기억력감퇴 증상을 치료하는 한약의 작용기전 중 AChE 효소활성억제효과는 忍冬藤 의 6종에서 나타났으며, 이는 전통적인 한방치료에 과학적인 기초자료를 제공할 것으로 생각한다.

Keywords

References

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