Interaction of Flavanols in Green Tea Extract during Heat Processing and Storage

Wang, Li-Fei;Kim, Dong-Man;Lee, Chang-Y.

  • Published : 20021200

Abstract

Epigallocatechin gallate (EGCG) is the most abundant but least stable flavanol in green tea extract. In this study, EGCG and ascorbic acid, the most common reducing agent used in foods, were respectively added to the green tea extract to investigate the chemical nature of EGCG and its interrelationship with other flavanols during processing and storage. Added with 0.65 mg EGCG/mL, the green tea extract turned much darker than the control after heat processing and 12 days of storage, but more other epicatechins were detected in the sample with additional EGCG. This could be due to the degradation of EGCG or the direct antioxidant effect of EGCG on the autooxidation of other epicatechins. On the other hand, ascorbic acid (0.4%) played some level of antioxidant function against the autooxidation of green tea flavanols, and also significantly inhibited the isomerization between epicatechins and catechins caused by heat treatment. However, the extra color problems resulting from the oxidation of ascorbic acid itself as well as its reactions with other green tea compounds prevents it being a proper antibrowning agent for the production of green tea beverage.

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References

  1. ACS Sym. Ser. No. 507 Protection against tobacco-specific, nitrosamine-induced lung tumorigenesis by green tea and its components;Phenolic compounds in food and their effects on health II Chung, F.;Xu, Y.;Ho, C.;Desai, D.;Han, C.;Huang, M.(ed.);Ho, C.(ed.);Lee, C.Y.(ed.)
  2. Food Sci. and Technol. v.9 Are tannins a double-edged sword in biology and health? Chung, K.;Wei, C.;Johnson, M.C.
  3. ACS Sym. Ser. No. 507 Inhibitory effect of green tea on tumorigenesis and tumor growth in mouse skin;Phenolic compounds in food and their effects on health II Conney, A.H.;Wang, Z.Y.;Ho, C.;Yang, C.S.;Huang, M.
  4. Nippon Nogeikagaku Kaoshi v.59 Antioxidative activity of tea leaf catechins Matsuzaki, T.;Hara, Y.
  5. ACS Sym. Ser. No. 507 Phenolic antioxidants as inducers of anticarcinogenic enzymes;Phenolic compounds in food and their effects on health II Prochaska, H.J.;Talalay, P.;Huang, C.(ed.);Ho, C.(ed.);Lee, C.Y.(ed.)
  6. Agric. Biol. Chem. v.53 Antibacterial substances in Japanese green tea extract against Streptococcus mutans, a carcinogenic bacterium Sakanaka, S.;Kim, M.;Taniguchi, M.;Yamamoto, T.
  7. ACS Sym. Ser. No. 507 Inhibition of nitrosamine-induced tumorigenesis by green tea and black tea;Phenolic compounds in food and their effects on health II Wang, Z.Y.;Hong, J.;Huang, M.;Conney, A.H.;Yang, C.S.;Huang, C.(ed.);Ho, C.(ed.);Lee, C.Y.(ed.)
  8. Journal of Agricultural and Food Chemistry v.48 no.9 Effects of heat processing and storage on flavanols and sensory qualities of green tea beverage Wang, L.;Kim, D.;Lee, C.Y. https://doi.org/10.1021/jf0003597
  9. Food Reviews International v.11 no.3 Special issue on tea Yamanishi, T.;Hara, Y.;Luo, S.;Wickremasinghe, R.L. https://doi.org/10.1080/87559129509541049
  10. ACS Sym. Ser. No. 507 Manufacturing and chemistry of tea;Phenolic compounds in food and their effects on health I Balentine, D.A.;Huang, C.(ed.);Ho, C.(ed.);Lee, C.Y.(ed.)
  11. ACS Sym. Ser. No. 507 Catechins of green tea-antioxidant activity;Phenolic compounds in food and their effects on health II Lunder, T.L.;Huang, C.(ed.);Ho, C.(ed.);Lee, C.Y.(ed.)
  12. Bioscience Biotechnology and Biochemistry v.61 no.9 Preparation of epimers of tea catechins by heat treatment Seto, R.;Nakamura, H.;Nanjo, F.;Hara, Y. https://doi.org/10.1271/bbb.61.1434
  13. Journal of Food Science v.64 no.5 Degradation kinetics of (-)-epigallocatechin gallate as a function of pH and dissolved oxygen in liquid model system Zimeri, J.;Tong, C.H. https://doi.org/10.1111/j.1365-2621.1999.tb15905.x
  14. Journal of the Science of Food and Agriculture v.63 no.4 The role of (-)-epicatechin and polyphenol oxidase in the coupled oxidative breakdown of theaflavins Opie, S.C.;Clifford, M.N.;Robertson, A. https://doi.org/10.1002/jsfa.2740630409
  15. Nippon Shokuhin Kogyo Gakkaishi v.39 Effects of pH on stability of constituents in canned tea drinks Suematsu, S.;Hisanobu, Y.;Saigo, H.;Matsuda, R.;Hara, K.
  16. Journal of Agricultural and Food Chemistry v.45 no.12 Stability of green tea catechins Zhu, Q.Y.;Zhang, A.;Tsang, D.;Huang, Y.;Chen, Z. https://doi.org/10.1021/jf9706080