Korean journal of dermatology (대한피부과학회지)

Korean Dermatological Association (대한피부과학회)
권호 표지

Expression of MHC Class I, II and CD1 in the Vascular Endothelial Cells and Dermal Inflammatory Cells of Leprosy Skin Lesions

나병 병변의 혈관내피세포와 염증세포에서의 MHC class I, II와 CD1의 발현

Kim, Seong-Hyun;Choi, Hae-Young;Hahm, Jeong-Hee;Seong, Soon-Hee;Ko, Young-Hoon
김성희;최혜영;함정희;성순희;고영훈

  • Published : 20020600
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Abstract

Background : The recent experimental observations suggested that location of M. leprae in the Schwann cells was mediated by epineurial and endoneurial endothelial cells, giving M. leprae access to the inner compartment of nerves and thus to Schwann cells. CD1 is a family of nonpolymorphic ${\beta}_2$-microglobulin-associated transmembrane glycoproteins that is structurally related to classical MHC Ag-presenting molecules, but is encoded by a separate genetic locus. Recent reports have described that human T cells specifically recognize foreign lipid and glycolipid antigens presented by CD1 proteins. Thus, it appeared likely that CD1 represents the key component of a MHC - independent pathway for antigen presentation to T cells. Objective : we observed expression of antigen presenting molecules, such as MHC class I, II and CD1, in the vascular endothelial cells and inflammatory cells of leprosy skin lesion. Methods : MHC class I, II and CD1 expression were studied using immunohistochemical stains. Results : 1. In immunohistochemical stain of HLA-A, B, C, the level of expression in vascular endothelial cells of the borderline tuberculoid leprosy is higher than that of borderline lepromatous leprosy, but lower than that of normal skin tissue. 2. In HLA-A,B,C expression of the inflammatory cells, the level of borderline tuberculoid leprosy is higher than that of borderline lepromatous leprosy and of normal skin tissue(p>0.05). 3. Expression levels of HLA-DP, DQ, DR on endothelial cells decrease significantly in order of normal tissue, borderline tuberculoid leprosy, borderline lepromatous leprosy, lepromatous leprosy(p<0.05). 4. Expression levels of HLA-DP, DQ, DR on inflammatory cells decrease in order of lepromatous leprosy, borderline lepromatous leprosy, borderline tuberculoid leprosy, normal tissue, but statistical significance did not exist. 5. In immunohistochemical stains of CD1b, 3 sections of all 4 normal skin sections and 1 section of 3 borderline tuberculoid leprosy sections showed focal positivity on the dermal inflammtory cells, but borderline lepromatous leprosy sections did not show any positive inflammatory cells. 6. Epidermal Langerhans cells showed positivity on immunohistochemical stains of CD1a and CD1b. Conclusion : These results suggest that expression of MHC class I and II on the vascular endothelial cells and expression of CD1b on the inflammatory cells decrease in order of immunity of lepromatous skin lesion and that vascular enothelial cells play an important role in the pathogenesis of leprosy.

Keywords

References

  1. Scollard DM. Endothelial cells and the pathogenesis of lepromatous neuritis: insights from armadillo model. Microbes Inf 2000;2:1835-1843 https://doi.org/10.1016/S1286-4579(00)01335-6
  2. Scollard DM, McCormick G and Allen JL. Localization of M. leprae to endothelial cells of epineurial and perineurial blood vessels and lymphatics. Am J Pathol 1999;154:1611-1620 https://doi.org/10.1016/S0002-9440(10)65414-4
  3. Fite GL. The vascular lesions of leprosy. Int J Lepr 1941:9:193-202
  4. Courth G, McDougall AC. Untreated lepromatous leprosy : Histopathological fmdings in cutaneous blood vessels. Int J Lepr 1979;47:500-501
  5. Burchard PJ, Bierther M. An electron microscopic study of small cutaneous vessels in lepromatous leprosy. Int J Lepr 1985;53:70-74
  6. Job CK. Pathology of leprosy. In: Hastings RC RC(Ed.) Leprosy. 4th ed. Churchill Livingstone, 1994:193-224
  7. Scollard DM. Association of Mycobacterium leprae with human endothelial cells in vitro. Lab Invest 2000;80:663-669 https://doi.org/10.1038/labinvest.3780069
  8. Antunes SL, Motta E, de Almeida SM, Gallo ME, Nery JA, Lenzi HL. Distinct patterns of microvasculature in the cutaneous lesions of leprosy. Int J Lepr Other Mycobact Dis 2000;68:143-151
  9. Salgarne PR, Mahaevan PR, Antia NH. Mechanism of immunosuppression in leprosy: presence of suppressor factor(s) from macrophages of lepromatous patients. Infect Immun 1983;40:1119-1126
  10. Sibley LD, Krahenbuhl JL. Induction of unresponsiveness to gamma interferon in macrophages infected with Mycobacterium leprae. Infect Immun 1988;56:1912-1919
  11. Nath I, Vemuri N, Reddi AL, Jain S, Brooks P, Colston MJ, et al. The effect of antigen presenting cells on the cytokine profiles of stable and reactional lepromatous leprosy patients. Immunol Lett 2000;75:69-76 https://doi.org/10.1016/S0165-2478(00)00271-6
  12. Sieling PA, Chatterjee D, Porcelli SA, Prigozy TI, Mazzaccaro RJ, Soriano T, et al. CDl-restricted T cell recognition of microbial lipoglycan antigens. Science 1995;269:185-186 https://doi.org/10.1126/science.7542402
  13. 이성범，김세곤，이혜정，신항계，김종필，고영훈 등. 나균에 감염된 대식세포에서의 Interferon-r의 interleukin-10 분비 억제를 통한 CD1 발현 증가에 대한 연구. 대한나학회지 2000;33:47-54
  14. Pober JS, Kluger MS, Schechner JS. Human endothelial cell presentation of antigen and the homing of memory/effector T cells to skin. Ann N Y Acad Sci 2001;941:12-25 https://doi.org/10.1111/j.1749-6632.2001.tb03706.x
  15. Sieling PA, Jullien D, Dahlemm M, Tedder TF, Rea TH, Modlin RL, et al. CD1 expression by dendritic cells in human leprosy lesions: correlation with effective host immunity. J Immunol 1999;162:1851-1858
  16. Pamer E, Wang CR, Flaherty L, Lindahl KF, Bevan MJ. H-2M3 presents a Listeria monocytogenes peptide to cytotoxic T lymphocytes. Cell 1992;70:215-223 https://doi.org/10.1016/0092-8674(92)90097-V
  17. Braud V, Jones EY, McMichael A. The human major histocompatibility complex class Ib molecule HLA-E binds signal sequence-derived peptides with primary anchor residues at positions 2 and 9. Eur J Immunol 1997;27:1164-1169 https://doi.org/10.1002/eji.1830270517
  18. Porcelli S, Brenner MB, Greenstein JL, Balk SP, Terhorst C, Bleicher PA. Recognition of cluster of differentiation 1 antigens by human CD4-CD8- cytotoxic T lymphocytes. Nature 1989;341:447-450 https://doi.org/10.1038/341447a0
  19. Porceli S, Morita CT, Brenner MB. CDlb restricts the response of human CD4-8- T lymphocytes to a microbial antigen. Nature 1992;360:593-597 https://doi.org/10.1038/360593a0
  20. Beckman EM, Porcelli SA, Morita CT, Behar SM, Furlong ST, Brenner MB. Recognition of a lipid antigen by CDl-restricted alpha beta+ T cells. Nature 1994;372:691-694 https://doi.org/10.1038/372691a0
  21. Flynn JL, Goldstein MM, Triebold KJ, Koller B, Bloom BR. Major histocompatibility complex class I-restricted T cells are required for resistance to Mycobacterium tuberculosis infection. Proc Natl Acad Sci U S A 1992;89:12013-12017 https://doi.org/10.1073/pnas.89.24.12013
  22. Orme IM, Collins FM. Protection against Mycobacterium tuberculosis infection by adoptive immunotherapy. Requi rement for T cell-deficient recipients. J Exp Med 1983;158:74-83 https://doi.org/10.1084/jem.158.1.74