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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Short-term Administration of Conjugated Linoleic Acid Reduces Liver Triglyceride Concentration and Phosphatidate Phosphohydrolase Activity in OLETF Rats

  • Rahman, Shaikh Mizanoor (Department of Applied Biological Sciences, Saga University) ;
  • Huda, M. Nazmul (Department of Biochemistry and Molecular Biology, University of Dhaka) ;
  • Uddin, M. Nas (Department of Biochemistry and Molecular Biology, University of Dhaka) ;
  • Akhteruzzaman, Sharif (Department of Biochemistry and Molecular Biology, University of Dhaka)
  • Published : 2002.09.30
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Abstract

The present study explored the short-term effects of dietary conjugated-linoleic acid (CLA) on liver lipid metabolism in starved/refed Otsuka Long Evans Tokushima Fatty (OLETF) rats. Male OLETF rats (12 weeks old) were starved for 24 hours, then refed for 48 hours with either a CLA diet [7.5% CLA and 7.5% Safflower oil (SAF)] or a SAF control diet (15% SAF). The results demonstrated a 30% reduction of hepatic triglyceride (TG) concentration in the CLA group when compared to the control group. Liver cholesterol concentration was also 26% lower in the CLA fed rats. The activity of mitochondrial carnitine palmitoyltransferase, the rate-limiting enzyme of fatty acid oxidation, was moderately elevated by 1.2-fold in the livers of the CLA group when compared to the control. In contrast, phosphatidate phosphohydrolase, the rate-limiting enzyme for TG synthesis, was found to be 20% lower in the livers of the CLA-fed rats. Therefore, dietary CLA evidently lowers liver lipid concentrations through a reduced TG synthesis and enhanced fatty acid oxidation in starved/refed OLETF rats.

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References

  1. Baltzell, J. K. and Berdanier, C. D. (1985) Effect on the interaction of dietary carbohydrate and fat on the responses of rats to starvation/refeeding. J. Nutr. 115, 104-110
  2. Belury, M. A. and Kempa-Steczko, A. (1997) Conjugated linoleic acid modulates hepatic lipid composition in mice. Lipids 32, 199-204. https://doi.org/10.1007/s11745-997-0025-0
  3. Cha, J. Y., Yamagata, Y., Yamamoto, K., Oogani, K. and Yanagita, T. (1998) Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phosphatide phosphorylase activity in rats. Biosci. Biofech. Biochem. 62, 508-513. https://doi.org/10.1271/bbb.62.508
  4. Chin, S. F., Liu, W., Storkson, J. M., Ha, Y. L. and Pariza, M. W. (1992) Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Compos. Anal. 5, 185-197. https://doi.org/10.1016/0889-1575(92)90037-K
  5. Fisher, R. A. (1970) Statistical methods for research workers. Vol.14, pp.140-142. Oliver and Boyd, Edinburgh, U.K
  6. Gavino, V. C., Gavino, G., Leblanc, M. J. and Tuchweber, B. (2000) An isomeric mixture of conjugated linoleic acids but not pure cis-9, trans-11-octadecadienoic acid affects body weight gain and plasma lipids in hamsters. J. Nutr. 130, 27-29
  7. Gomez-Munoz, A., Hatch, G. M., Martin, A., Jamal, Z., Vance, D. E. and Brindley, D. N. (1992) Effect of Okadaic acid on the activities of two distinct phosphatidate phosphohydrolase in rat hepatocytes. FEBS Lett. 301, 103-106. https://doi.org/10.1016/0014-5793(92)80219-7
  8. Ha, Y. L., Grimm, N. K. and Pariza, M. W. (1989) Newly-recognized anticarcinogenic fatty acids: identification and quantification in natural and processed cheeses. J. Agri. Food Chem. 37, 75-81. https://doi.org/10.1021/jf00085a018
  9. Halminski, M. A., Marsh, J. B. and Harrison, E. H. (1991) Differential effects of fish oil, safflower oil and palm oil on fatty acid oxidation and glycerlipid synthesis in rat liver. J. Nutr. 121, 1554-1561.
  10. Houseknecht, K. L., Heuvel, J. P. V., Moya-Camarena, S. Y., Portocarrero, C. P, Peck, L. W., Nickel, K. P. and Belury, M. A. (1998) Dietary conjugated linoleic acid normalizes impaired glucose tolerance in the Zucker diabetic fa/fa rat. Biochem. Biophys. Res. Commun. 244, 678-682. https://doi.org/10.1006/bbrc.1998.8303
  11. Ikeda, I., Cha, J. Y., Yanagita, T., Nakatani, N., Oogami, K., lmaizumi, K. and Yazawa, K. (1998) Effects of dietary alpha-linoleic, eicosapentaenoic and decosahexaenoic acids on hepatic lipogenesis and beta-oxidation in rats. Biosci. Biotech. Biochem. 62, 675-680. https://doi.org/10.1271/bbb.62.675
  12. lp, C., Singh, M., Thompson, H. J. and Scimeca, J. A. (1991) Conjugated linoleic acid suppresses mammary carcinogenesis and proliferative activity of the mammary gland in rat. Cancer Res. 54, 1212-1215.
  13. lp, C., Scimeca, J. A. and Thompson, H. J. (1994) Conjugated linoleic acid, a powerful anticarcinogen from animal fat sources. Cancer 74, 1050-1054. https://doi.org/10.1002/1097-0142(19940801)74:3+<1050::AID-CNCR2820741512>3.0.CO;2-I
  14. Jamal, Z., Martin, A., Gomez-Munoz, A., Hales, P., Chang, P., Russell, J. C. and Brindley, D. N. (1992) Phosphatidate phosphohydrolase in the liver, heart and adipose tissue of the JCR: LA Corpulant rat and the lean genotypes: implications for glycerolipid synthesis and signal transduction. Int. J. Obesity 16, 789-799.
  15. Kawano, K., Hirashima, T., Mori, S., Saitoh, Y., Kuroshima, M. and Natori, T. (1992) Spontaneous long-term hyperglycemic rat with diabetic complications: Otsuka Long-Evans Tokushima Fatty (OLETF) strain. Diabetes 41, 1422-1428. https://doi.org/10.2337/diabetes.41.11.1422
  16. Kawano, K., Hirashima, T., Mori, S. and Natori, T. (1994) Otsuka Long-Evans Tokushima Fatty (OLETF) rat: a new NIDDM rat strain. Diabetes Res. Clin. Practice 24, S317 -S320. https://doi.org/10.1016/0168-8227(94)90269-0
  17. Kochan, Z., Karbowska. J. and Swierczynski, J. (1997) Unusual increase of lipogenesis in rat whit adipose tissue after multiple cycles of starvation and refeeding. Metabolism 46, 10-17.
  18. Lee, K. N., Kritchevsky, D. and Pariza, M. W. (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 19-25. https://doi.org/10.1016/0021-9150(94)90034-5
  19. Lowry, O. H., Rosenbrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
  20. Matin, J. C., Gregire. S., Siess, M. H., Genty, M., Chardigny, J. M., Berdeaux, O., Juaneda, P and Sebedio, J. L. (2000) Effects of conjugated linoleic acid isomers towards lipid metabolizing enzymes in male wister rats. Lipids 35, 91-98. https://doi.org/10.1007/s11745-000-0499-9
  21. Moya-Camarena. S. Y., Heuvel, J. P. V. and Belury, M. A. (1999) Conjugated linoleic acid activates peroxisome proliferators-activated receptor alpha and beta subtypes but does not induce hepatic peroxisome proliferation in Sprague-dawley rate. Biochim. Biophys. Acta 1436, 331-342. https://doi.org/10.1016/S0005-2760(98)00121-0
  22. Nace, C. S. and Szepesi. B. (1976) Dietary fatty acids on the control of glucose-6-phosphate dehydrogenase and malic enzyme in the starvedrefed rats. J. Nutr. 106, 285-291.
  23. Owens, J. L., Thompson, D., Shah, N. and Digirolamo, M. (1979) Effects of fasting and refeeding in the rat on adipocyte metabolic functions and response to insulin. J. Nutr. 109, 1584-1591.
  24. Park, Y., Albright. K. J., Liu, W., Storkson, J. M., Cook, M. E. and Pariza. M. W. (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32. 853-858 https://doi.org/10.1007/s11745-997-0109-x
  25. Park, Y., Albright. K. J., Storkson, J. M., Cook, M. E. and Pariza, M. W. (1999) Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid. Lipids 34, 243-248. https://doi.org/10.1007/s11745-999-0359-7
  26. Pineda Torra, I., Gervois, P. and Staels, B. (1999) Peroxisome proliferator-activated receptor alpha in metabolic disease, inflammation, atherscerosis and aging. Curr. Opin. Lipidol. 10, 151-159. https://doi.org/10.1097/00041433-199904000-00009
  27. West, D. B., DeLany. J. P., Carnet, P. M., Blohm, F., Truett, A. A. and Scimeca. J. (1998) Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am. J. Physiol. 275. R667-R672.

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