Marker-assisted Introgression of Quantitative Trait Loci Associated with Plant Regeneration Ability in Anther Culture of Rice (Oryza sativa L.)

Yong-Shin Kwon;Kyung-Min Kim;Kim, Do-Hoon;Moo-Young Eun;Jae-Keun Sohn

  • Published : 20020000

Abstract

A marker-assisted selection (MAS) breeding program was used to improve the plant regenerability of indica rice. A significant quantitative trait loci (QTL) that is associated with the capacity for green plant regenera-tion in the anther culture of rice was mapped on chromosome 10 using recombinant inbred (RI) popu-lation from Milyang 23/Gihobyeo. The marker that was chosen to follow the QTL region was used in MAS. This marker co-segregated with the regeneration abil-ity in F2 individuals that were derived from MGRI 079/IR 36. In order to clarify the relationship between this marker and plant regenerability, the backcross population was screened with a RFLP marker. The capacity of plant regeneration of the backcross popu-lation was clearly distinguished by the marker geno-type. The development of near isogenic line (NILs) with high regenerability through MAS will save time, labor, and cost in indica rice breeding.

Keywords

References

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