Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Immunohistochemical Localization of BcI-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis

Moon, Chang-Jong;Lee, Yong-Duk;Shin, Tae-Kyun

  • Published : 20020000
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Abstract

We examined the localization of the anti-apoptotic molecule BcI-2 in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis (EAE). Western blot analysis showed that BcI-2 was constitutively expressed in normal spinal cords, and weakly increased in response to complete Freund's adjuvant(CFA) immunization. In EAE, with infiltration of inflammatory cells into spinal cords, BcI-2 declined during the peak stage and further decreased during the recovery stage. Immunohistochemically, some neurons and glial cells constitutively expressed BcI-2 in normal rat spinal cords. In the spinal cords of rats with EAE, BcI-2 was also immunoreacted in some perivascular inflammatory cells while some brain cells, such as neurons and GFAP (+) astrocytes showed less BcI-2 immunoreaction. These findings suggest that in EAE, BcI-2 expression in the CNS host cells decreases with CNS inflammation, possibly progressing to cell death in some cases, while the survival of host cells, including neurons, astrocytes, and some inflammatory cells, is associated with activation of the anti-apoptotic molecule BcI-2. Taking all into considerations, its is postulated that BcI-2 either beneficially or detrimentally functions in some host cells depending on the activation stage of each cell type.

Keywords

References

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