Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Growth Inhibition by Connexin26 Expression in Cultured Rodent Tumor Cells

Hae-Jung Lee;In-Kyung Lee;Kyung-Hwan Seul;Seung-Keun Rhee

  • Published : 20020000
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Abstract

The Connexin (Cx) gene family acts as a tumor sup-pressor.However, it is unclear whether the tumor-suppressingactivity acquired by Cx gene transfectionis mainly due to the recovery of the gap junction-mediatedintercellular communication (GJIC) or toother unknown mechanisms. In order to elucidate themechanism of the Cx-induced tumor-suppressing ac-tivity,we transfected Cx26 cDNA into a rodent mam-marytumor cell-line (BICR-M1Rk) in which Cx43 hadbeen normally expressed and a typical pattern ofGJIC had been observed. The exogenous Cx26 wasmainly localized on the nuclear envelope, whereasmost of the endogenous Cx43 resided at the plasmamembrane of the transfected BICR-M1Rk. Consistentwith the localization of Cx26, GJIC was not increasedupon the transfection of Cx26 when it was assessed bya scrape-loading dye transfer technique. A conven-tional[ 3 H]-thymidine incorporation study showed thatthe growth rate of the Cx26-transfected cells was sig-nificantlydecreased (70%), compared to that of thecontrol BICR-M1Rk. Therefore, our results clearlydemonstrate that the exogenously expressed Cx26 inthe BICR-M1Rk cancer cell-line exerts an anti-proliferateactivity in a GJIC-independent manner.

Keywords

References

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