Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
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The Effect of Treatment with Imipramine and/or Tryptophan after Chronic Stress on the Serotonergic Immunoreactivity in Rat Raphe Nucleus

장기적인 스트레스후 imipramine과 tryptophan처리가 흰쥐 솔기핵의 serotonin 면역 반응에 미치는 영향

Kim, Myoung-Soon;Lee, Chang-Hyun
김명순;이창현

  • Published : 20021000
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Abstract

These experiments were performed to investigate the effect of saline, stress, imipramine, stress-imipramine and/or stress-tryptophan on serotonin immunoreactivity in raphe nucleus of the rats (200${\sim}$220 g, body weight). The animals were injected i.p. with imipramine (15 mg/kg) and tryptophan (15 mg/kg) after electric shocks for 20 days. The result by immunohistochemical methods were as follows; 1. Serotonin-immunoreactive neurons in the raphe nucleus of midbrain were significantly increased in stress treated group compared to saline treated group. 2. Serotonin-immunoreactive neurons in the raphe nucleus of midbrain were decreased in imipramine treated group compared all the other group. 3. Serotonin-immunoreactive neurons in the raphe nucleus of midbrain were significantly decreased in stress-imipramine treated group compared to stress alone treated group but were significantly increased in stress-imipramine treated group compared to imipramine treated group. 4. Serotonin-immunoreactive neurons in the raphe nucleus of midbrain were significantly increased in stress-tryptophan treated group compared to stress alone and saline treated group. These experiments indicated that serotonin immunoreactive neurons in raphe nucleus of midbrain were increased due to the activation of stress and decreased by suppresing activation of stress through imipramine treatment.

전기 충격으로 stress를 가한 후 imipramine과 tryptophan을 흰쥐의 복강에 투여한 20일후 연수와 중뇌 솔기핵의 신경세포체의 수와 염색강도를 면역조직화학 염색법에 의하여 관찰한 바 다음과 같은 결과를 얻었다. 1. Stress를 처리한 군에서는 saline을 투여한 군에 비해 serotonin에 면역반응을 보인 신경 세포체의 수가 유의성있게 증가함을 알 수 있었다. 2. Imipramine을 투여한 군에서는 saline 투여군에 비해 serotonin에 대한 면역 반응을 보인 신경 세포체의 수가 감소 되었다. 3. Stress-imipramine 처리군에서는 stress 단독 처리군에 비하여 serotonin에 면역반응을 보인 신경 세포체의 수는 유의성 있게 감소하였으나 imipramine 처리군보다는 유의성있게 증가하였다. 4. Stress-tryptophan 처리군에서는 stress단독 처리군이나 saline 처리군에 비하여 serotonin에 면역반응을 보인 신경 세포체의 수는 유의성있게 증가하였다. 이상의 실험결과로 stress로 인하여 중뇌 솔기핵의 serotonin에 면역 반응을 보인 신경 세포체들이 활성화되어 증가되었으나 imipramine과 병용해서 처리할 때는 imipramine에 의하여 serotonin에 면역 반응을 보인 신경 세포체의 활성이 억제됨을 알 수 있었다.

Keywords

References

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