The Suitable Cultural Conditions for Inoculum Production of Epicoccosorus nematosporus as a Mycoherbicide Agent

미생물제초제 올방개지문무늬병균의 균총형성 및 포자형성에 관여하는 요인

Hong, Yeon-Kyu;Hyun, Jong-Nae;Ryu, Kil-Lim;Shin, Dong-Bum;Kim, Soon-Chul
홍연규;현종내;류길림;신동범

  • Published : 20020000

Abstract

Cultural conditions affecting mycelial growth and conidiation of Epicoccosorus nematosporus as a pontential mycoherbicide agent were evaluated. Mycelia grew well on four kinds of natural media such as potato dextrose agar, oatmeal agar, wheat bran agar, and rice polish agar, and a semi-synthetic media (EP complete agar). Optimum temperature and pH range for the growth of the fungus was 20-24$^{\circ}C$ and pH 5.0 to 6.0, respectively. Carbon sources affected mycelial growth of E. nematosporus. Glucose was the most effectively utilized carbohydrate, followed by fructose and sucrose. E. nematosporus utilized a wide range of nitrogen sources:arginine and serine were the most effective. Conidia were produced on oatmeal agar and greatly stimulated by continuous radiation with fluorescent light for 2 or 3 days at 28$^{\circ}C$ with 60% relative humidity. On oatmeal agar, $10^9$conidia per plate were produced within 48 h

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